Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice
Herpetic stromal keratitis is caused by ocular infection with herpes simplex virus type 1 (HSV-1) and constitutes a leading cause of human blindness. The effect of meliacine, an antiviral compound isolated from leaves of Melia azedarach L. that inhibits HSV-1 replication in vitro, was examined on ex...
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todo:paper_01466615_v61_n4_p474_Alche2023-10-03T15:00:14Z Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice Alché, L.E. Berra, A. Veloso, M.J. Coto, C.E. Herpes simplex virus type 1 Herpetic stromal keratitis Melia azedarach L Plant antiviral antivirus agent meliacine unclassified drug animal experiment animal model animal tissue antimicrobial therapy antiviral activity article controlled study drug effect female herpes simplex keratitis Herpes simplex virus 1 histopathology male mouse nonhuman plant leaf scoring system virus isolation virus titration Herpetic stromal keratitis is caused by ocular infection with herpes simplex virus type 1 (HSV-1) and constitutes a leading cause of human blindness. The effect of meliacine, an antiviral compound isolated from leaves of Melia azedarach L. that inhibits HSV-1 replication in vitro, was examined on experimental corneal HSV-1 inoculation in Balb/c mice. Mice were inoculated with HSV-1 strain KOS at their corneas after abrasion. Meliacine was administered topically 3 times a day for 4 days beginning 1 day before inoculation. Infected animals treated or not with meliacine were observed carefully for the development of stromal keratitis and the clinical scoring was done 14 days post-infection. Histological examination of corneas and viral isolation from eyes from HSV-1 infected mice treated or not with meliacine were also carried out. It was found that the treatment of HSV-1- induced ocular disease in Balb/c mice with meliacine reduced significantly the development of clinical disease, as well as the histological damage in corneas. The viral titers detected in eyes of infected and treated mice were 2-orders-of-magnitude lower than those corresponding to HSV-1 infected control animals. Mock-infected and treated mice did not reveal any corneal alteration due to the administration of the compound. Meliacine was found to exert a strong antiviral action on HSV-1-induced ocular disease in mice with no evidence of toxic effects. (C) 2000 Wiley-Liss, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01466615_v61_n4_p474_Alche |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Herpes simplex virus type 1 Herpetic stromal keratitis Melia azedarach L Plant antiviral antivirus agent meliacine unclassified drug animal experiment animal model animal tissue antimicrobial therapy antiviral activity article controlled study drug effect female herpes simplex keratitis Herpes simplex virus 1 histopathology male mouse nonhuman plant leaf scoring system virus isolation virus titration |
| spellingShingle |
Herpes simplex virus type 1 Herpetic stromal keratitis Melia azedarach L Plant antiviral antivirus agent meliacine unclassified drug animal experiment animal model animal tissue antimicrobial therapy antiviral activity article controlled study drug effect female herpes simplex keratitis Herpes simplex virus 1 histopathology male mouse nonhuman plant leaf scoring system virus isolation virus titration Alché, L.E. Berra, A. Veloso, M.J. Coto, C.E. Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| topic_facet |
Herpes simplex virus type 1 Herpetic stromal keratitis Melia azedarach L Plant antiviral antivirus agent meliacine unclassified drug animal experiment animal model animal tissue antimicrobial therapy antiviral activity article controlled study drug effect female herpes simplex keratitis Herpes simplex virus 1 histopathology male mouse nonhuman plant leaf scoring system virus isolation virus titration |
| description |
Herpetic stromal keratitis is caused by ocular infection with herpes simplex virus type 1 (HSV-1) and constitutes a leading cause of human blindness. The effect of meliacine, an antiviral compound isolated from leaves of Melia azedarach L. that inhibits HSV-1 replication in vitro, was examined on experimental corneal HSV-1 inoculation in Balb/c mice. Mice were inoculated with HSV-1 strain KOS at their corneas after abrasion. Meliacine was administered topically 3 times a day for 4 days beginning 1 day before inoculation. Infected animals treated or not with meliacine were observed carefully for the development of stromal keratitis and the clinical scoring was done 14 days post-infection. Histological examination of corneas and viral isolation from eyes from HSV-1 infected mice treated or not with meliacine were also carried out. It was found that the treatment of HSV-1- induced ocular disease in Balb/c mice with meliacine reduced significantly the development of clinical disease, as well as the histological damage in corneas. The viral titers detected in eyes of infected and treated mice were 2-orders-of-magnitude lower than those corresponding to HSV-1 infected control animals. Mock-infected and treated mice did not reveal any corneal alteration due to the administration of the compound. Meliacine was found to exert a strong antiviral action on HSV-1-induced ocular disease in mice with no evidence of toxic effects. (C) 2000 Wiley-Liss, Inc. |
| format |
JOUR |
| author |
Alché, L.E. Berra, A. Veloso, M.J. Coto, C.E. |
| author_facet |
Alché, L.E. Berra, A. Veloso, M.J. Coto, C.E. |
| author_sort |
Alché, L.E. |
| title |
Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| title_short |
Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| title_full |
Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| title_fullStr |
Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| title_full_unstemmed |
Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| title_sort |
treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice |
| url |
http://hdl.handle.net/20.500.12110/paper_01466615_v61_n4_p474_Alche |
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