Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.

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Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fra...

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Autores principales:	Parussini, F., Duschak, V.G., Cazzulo, J.J.
Formato:	JOUR
Materias:	cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01455680_v44_n3_p513_Parussini
record_format	dspace
spelling	todo:paper_01455680_v44_n3_p513_Parussini2023-10-03T15:00:02Z Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. Parussini, F. Duschak, V.G. Cazzulo, J.J. cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite. Fil:Parussini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi
spellingShingle	cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi Parussini, F. Duschak, V.G. Cazzulo, J.J. Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
topic_facet	cysteine proteinase isoenzyme animal article cell membrane enzymology metabolism Trypanosoma cruzi Animals Cell Membrane Cysteine Endopeptidases Isoenzymes Trypanosoma cruzi
description	Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.
format	JOUR
author	Parussini, F. Duschak, V.G. Cazzulo, J.J.
author_facet	Parussini, F. Duschak, V.G. Cazzulo, J.J.
author_sort	Parussini, F.
title	Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_short	Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_full	Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_fullStr	Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_full_unstemmed	Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_sort	membrane-bound cysteine proteinase isoforms in different developmental stages of trypanosoma cruzi.
url	http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
work_keys_str_mv	AT parussinif membraneboundcysteineproteinaseisoformsindifferentdevelopmentalstagesoftrypanosomacruzi AT duschakvg membraneboundcysteineproteinaseisoformsindifferentdevelopmentalstagesoftrypanosomacruzi AT cazzulojj membraneboundcysteineproteinaseisoformsindifferentdevelopmentalstagesoftrypanosomacruzi
_version_	1807321346552627200

Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.

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