Sequence-specific DNA modification in Acetobacter xylinum.
Two cryptic plasmids have been discovered in Acetobacter xylinum B42 and in its derivative PEA-1, a cellulose defective mutant. These two plasmids were designated pAX1 and pAX2 (50 and 105 kb in size, respectively). A restriction map was constructed for pAX1. Attempts to cure these plasmids were uns...
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p759_Petroni |
| Aporte de: |
| id |
todo:paper_01455680_v42_n5_p759_Petroni |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01455680_v42_n5_p759_Petroni2023-10-03T14:59:58Z Sequence-specific DNA modification in Acetobacter xylinum. Petroni, E.A. Bocca, S.N. Ielpi, L. bacterial DNA endodeoxyribonuclease ApoI type II site specific deoxyribonuclease article genetics Gluconacetobacter xylinus isolation and purification mutation nucleotide sequence plasmid restriction mapping Base Sequence Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific DNA, Bacterial Gluconacetobacter xylinus Mutation Plasmids Restriction Mapping Two cryptic plasmids have been discovered in Acetobacter xylinum B42 and in its derivative PEA-1, a cellulose defective mutant. These two plasmids were designated pAX1 and pAX2 (50 and 105 kb in size, respectively). A restriction map was constructed for pAX1. Attempts to cure these plasmids were unsuccessful. Enzyme restriction analysis showed that these plasmids contain protected EcoRI and ApoI sites. Using Southern blot and hybridization techniques, the protection was extended to chromosomal DNA. Enzyme restriction analysis of several plasmids, from different origins and containing different incompatibility groups, isolated from strain PEA-1 also showed EcoRI and ApoI protection. The presence of modifications on specific sequences was not found in A. xylinum 8747. These results strongly suggest the presence of a modification system in A. xylinum B42 that recognizes the tetranucleotide 5'-AATT. Fil:Petroni, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bocca, S.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p759_Petroni |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial DNA endodeoxyribonuclease ApoI type II site specific deoxyribonuclease article genetics Gluconacetobacter xylinus isolation and purification mutation nucleotide sequence plasmid restriction mapping Base Sequence Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific DNA, Bacterial Gluconacetobacter xylinus Mutation Plasmids Restriction Mapping |
| spellingShingle |
bacterial DNA endodeoxyribonuclease ApoI type II site specific deoxyribonuclease article genetics Gluconacetobacter xylinus isolation and purification mutation nucleotide sequence plasmid restriction mapping Base Sequence Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific DNA, Bacterial Gluconacetobacter xylinus Mutation Plasmids Restriction Mapping Petroni, E.A. Bocca, S.N. Ielpi, L. Sequence-specific DNA modification in Acetobacter xylinum. |
| topic_facet |
bacterial DNA endodeoxyribonuclease ApoI type II site specific deoxyribonuclease article genetics Gluconacetobacter xylinus isolation and purification mutation nucleotide sequence plasmid restriction mapping Base Sequence Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific DNA, Bacterial Gluconacetobacter xylinus Mutation Plasmids Restriction Mapping |
| description |
Two cryptic plasmids have been discovered in Acetobacter xylinum B42 and in its derivative PEA-1, a cellulose defective mutant. These two plasmids were designated pAX1 and pAX2 (50 and 105 kb in size, respectively). A restriction map was constructed for pAX1. Attempts to cure these plasmids were unsuccessful. Enzyme restriction analysis showed that these plasmids contain protected EcoRI and ApoI sites. Using Southern blot and hybridization techniques, the protection was extended to chromosomal DNA. Enzyme restriction analysis of several plasmids, from different origins and containing different incompatibility groups, isolated from strain PEA-1 also showed EcoRI and ApoI protection. The presence of modifications on specific sequences was not found in A. xylinum 8747. These results strongly suggest the presence of a modification system in A. xylinum B42 that recognizes the tetranucleotide 5'-AATT. |
| format |
JOUR |
| author |
Petroni, E.A. Bocca, S.N. Ielpi, L. |
| author_facet |
Petroni, E.A. Bocca, S.N. Ielpi, L. |
| author_sort |
Petroni, E.A. |
| title |
Sequence-specific DNA modification in Acetobacter xylinum. |
| title_short |
Sequence-specific DNA modification in Acetobacter xylinum. |
| title_full |
Sequence-specific DNA modification in Acetobacter xylinum. |
| title_fullStr |
Sequence-specific DNA modification in Acetobacter xylinum. |
| title_full_unstemmed |
Sequence-specific DNA modification in Acetobacter xylinum. |
| title_sort |
sequence-specific dna modification in acetobacter xylinum. |
| url |
http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p759_Petroni |
| work_keys_str_mv |
AT petroniea sequencespecificdnamodificationinacetobacterxylinum AT boccasn sequencespecificdnamodificationinacetobacterxylinum AT ielpil sequencespecificdnamodificationinacetobacterxylinum |
| _version_ |
1807324059614052352 |