Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli.
The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in po...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri |
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todo:paper_01455680_v42_n5_p711_Nastri2023-10-03T14:59:57Z Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. Nastri, H.G. Algranati, I.D. bacterial protein bacterial RNA kanamycin kinase messenger RNA phosphotransferase polyamine putrescine antibiotic resistance article bacterial gene biosynthesis drug effect enzymology Escherichia coli gene expression genetic transformation genetics kinetics metabolism plasmid Bacterial Proteins Escherichia coli Gene Expression Genes, Bacterial Kanamycin Kinase Kanamycin Resistance Kinetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Polyamines Putrescine RNA, Bacterial RNA, Messenger Transformation, Genetic The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in polyamine-supplemented bacteria than in those depleted of the organic bases. Concomitantly, we have observed that the increase of kanamycin phosphotransferase activity evoking the antibiotic resistance was higher in bacteria cultivated in the presence of putrescine. This result seems to depend exclusively on the enhanced capacity of the translation process in bacteria grown with polyamines since the transcription of phosphotransferase gene was higher in cells subjected to polyamine starvation. Fil:Nastri, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial protein bacterial RNA kanamycin kinase messenger RNA phosphotransferase polyamine putrescine antibiotic resistance article bacterial gene biosynthesis drug effect enzymology Escherichia coli gene expression genetic transformation genetics kinetics metabolism plasmid Bacterial Proteins Escherichia coli Gene Expression Genes, Bacterial Kanamycin Kinase Kanamycin Resistance Kinetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Polyamines Putrescine RNA, Bacterial RNA, Messenger Transformation, Genetic |
| spellingShingle |
bacterial protein bacterial RNA kanamycin kinase messenger RNA phosphotransferase polyamine putrescine antibiotic resistance article bacterial gene biosynthesis drug effect enzymology Escherichia coli gene expression genetic transformation genetics kinetics metabolism plasmid Bacterial Proteins Escherichia coli Gene Expression Genes, Bacterial Kanamycin Kinase Kanamycin Resistance Kinetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Polyamines Putrescine RNA, Bacterial RNA, Messenger Transformation, Genetic Nastri, H.G. Algranati, I.D. Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| topic_facet |
bacterial protein bacterial RNA kanamycin kinase messenger RNA phosphotransferase polyamine putrescine antibiotic resistance article bacterial gene biosynthesis drug effect enzymology Escherichia coli gene expression genetic transformation genetics kinetics metabolism plasmid Bacterial Proteins Escherichia coli Gene Expression Genes, Bacterial Kanamycin Kinase Kanamycin Resistance Kinetics Phosphotransferases (Alcohol Group Acceptor) Plasmids Polyamines Putrescine RNA, Bacterial RNA, Messenger Transformation, Genetic |
| description |
The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in polyamine-supplemented bacteria than in those depleted of the organic bases. Concomitantly, we have observed that the increase of kanamycin phosphotransferase activity evoking the antibiotic resistance was higher in bacteria cultivated in the presence of putrescine. This result seems to depend exclusively on the enhanced capacity of the translation process in bacteria grown with polyamines since the transcription of phosphotransferase gene was higher in cells subjected to polyamine starvation. |
| format |
JOUR |
| author |
Nastri, H.G. Algranati, I.D. |
| author_facet |
Nastri, H.G. Algranati, I.D. |
| author_sort |
Nastri, H.G. |
| title |
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| title_short |
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| title_full |
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| title_fullStr |
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| title_full_unstemmed |
Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| title_sort |
effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in escherichia coli. |
| url |
http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p711_Nastri |
| work_keys_str_mv |
AT nastrihg effectofpolyaminesonplasmidmediatedkanamycinresistanceandkanamycinphosphotransferasegeneexpressioninescherichiacoli AT algranatiid effectofpolyaminesonplasmidmediatedkanamycinresistanceandkanamycinphosphotransferasegeneexpressioninescherichiacoli |
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1807318742121578496 |