delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.

There is evidence that delta-aminolevulinic acid (ALA), a precursor of porphyrin biosynthesis, and gamma-aminobutyric acid (GABA) would be incorporated into yeast cells by a common permease. The purpose of this work was to confirm this hypothesis and to identify the shared permease. The transport of...

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Autores principales:	Bermúdez Moretti, M., Correa García, S., Ramos, E., Batlle, A.
Formato:	JOUR
Materias:	4 aminobutyric acid 4 aminobutyric acid carrier aminolevulinic acid carrier protein GABA permease organic anion transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article enzyme specificity metabolism Saccharomyces cerevisiae Aminolevulinic Acid GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Proteins Substrate Specificity
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01455680_v42_n4_p519_BermudezMoretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01455680_v42_n4_p519_BermudezMoretti
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spelling	todo:paper_01455680_v42_n4_p519_BermudezMoretti2023-10-03T14:59:55Z delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4. Bermúdez Moretti, M. Correa García, S. Ramos, E. Batlle, A. 4 aminobutyric acid 4 aminobutyric acid carrier aminolevulinic acid carrier protein GABA permease organic anion transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article enzyme specificity metabolism Saccharomyces cerevisiae Aminolevulinic Acid GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Substrate Specificity There is evidence that delta-aminolevulinic acid (ALA), a precursor of porphyrin biosynthesis, and gamma-aminobutyric acid (GABA) would be incorporated into yeast cells by a common permease. The purpose of this work was to confirm this hypothesis and to identify the shared permease. The transport of GABA in Saccharomyces cerevisiae is mediated by three permeases: the general amino acid permease (GAP1), the specific proline permease (PUT4) and a fairly specific GABA permease (UGA4). To determine which of these permeases is also involved in ALA uptake, ALA and GABA incorporations were measured in strains lacking GAP1, UGA4 or GAP1 and UGA4 permeases. Results indicated that ALA is mainly incorporated by UGA4. This was also confirmed by regulatory studies, since ALA uptake was induced by GABA, and it is well known that UGA4 permease is induced by GABA. On the other hand, ALA did not induce the synthesis of this permease. Therefore, we demonstrate here that ALA, which cannot be used as a nitrogen source, is uptaken by S. cerevisiae cells mainly using a permease encoded by a gene subjected to a regulation typical of several nitrogen genes. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v42_n4_p519_BermudezMoretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	4 aminobutyric acid 4 aminobutyric acid carrier aminolevulinic acid carrier protein GABA permease organic anion transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article enzyme specificity metabolism Saccharomyces cerevisiae Aminolevulinic Acid GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Substrate Specificity
spellingShingle	4 aminobutyric acid 4 aminobutyric acid carrier aminolevulinic acid carrier protein GABA permease organic anion transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article enzyme specificity metabolism Saccharomyces cerevisiae Aminolevulinic Acid GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Substrate Specificity Bermúdez Moretti, M. Correa García, S. Ramos, E. Batlle, A. delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
topic_facet	4 aminobutyric acid 4 aminobutyric acid carrier aminolevulinic acid carrier protein GABA permease organic anion transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article enzyme specificity metabolism Saccharomyces cerevisiae Aminolevulinic Acid GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Substrate Specificity
description	There is evidence that delta-aminolevulinic acid (ALA), a precursor of porphyrin biosynthesis, and gamma-aminobutyric acid (GABA) would be incorporated into yeast cells by a common permease. The purpose of this work was to confirm this hypothesis and to identify the shared permease. The transport of GABA in Saccharomyces cerevisiae is mediated by three permeases: the general amino acid permease (GAP1), the specific proline permease (PUT4) and a fairly specific GABA permease (UGA4). To determine which of these permeases is also involved in ALA uptake, ALA and GABA incorporations were measured in strains lacking GAP1, UGA4 or GAP1 and UGA4 permeases. Results indicated that ALA is mainly incorporated by UGA4. This was also confirmed by regulatory studies, since ALA uptake was induced by GABA, and it is well known that UGA4 permease is induced by GABA. On the other hand, ALA did not induce the synthesis of this permease. Therefore, we demonstrate here that ALA, which cannot be used as a nitrogen source, is uptaken by S. cerevisiae cells mainly using a permease encoded by a gene subjected to a regulation typical of several nitrogen genes.
format	JOUR
author	Bermúdez Moretti, M. Correa García, S. Ramos, E. Batlle, A.
author_facet	Bermúdez Moretti, M. Correa García, S. Ramos, E. Batlle, A.
author_sort	Bermúdez Moretti, M.
title	delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
title_short	delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
title_full	delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
title_fullStr	delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
title_full_unstemmed	delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.
title_sort	delta-aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease uga4.
url	http://hdl.handle.net/20.500.12110/paper_01455680_v42_n4_p519_BermudezMoretti
work_keys_str_mv	AT bermudezmorettim deltaaminolevulinicaciduptakeismediatedbythegammaaminobutyricacidspecificpermeaseuga4 AT correagarcias deltaaminolevulinicaciduptakeismediatedbythegammaaminobutyricacidspecificpermeaseuga4 AT ramose deltaaminolevulinicaciduptakeismediatedbythegammaaminobutyricacidspecificpermeaseuga4 AT batllea deltaaminolevulinicaciduptakeismediatedbythegammaaminobutyricacidspecificpermeaseuga4
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delta-Aminolevulinic acid uptake is mediated by the gamma-aminobutyric acid-specific permease UGA4.

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