Regulation of putrescine uptake in Leishmania mexicana promastigotes.

Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to e...

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Detalles Bibliográficos
Autores principales: Gonzalez, N.S., Algranati, I.D.
Formato: JOUR
Materias:
cycloheximide
eflornithine
polyamine
putrescine
active transport
animal
article
drug effect
feedback system
growth, development and aging
kinetics
Leishmania mexicana
metabolism
Animals
Biological Transport, Active
Cycloheximide
Eflornithine
Feedback
Kinetics
Polyamines
Putrescine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01455680_v40_n7_p907_Gonzalez
record_format dspace
spelling todo:paper_01455680_v40_n7_p907_Gonzalez2023-10-03T14:59:55Z Regulation of putrescine uptake in Leishmania mexicana promastigotes. Gonzalez, N.S. Algranati, I.D. cycloheximide eflornithine polyamine putrescine active transport animal article drug effect feedback system growth, development and aging kinetics Leishmania mexicana metabolism Animals Biological Transport, Active Cycloheximide Eflornithine Feedback Kinetics Leishmania mexicana Polyamines Putrescine Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to exogenous putrescine or its derivative 1,4-dimethylputrescine. In contrast, spermidine, spermine, diaminopropane and cadaverine were unable to decrease putrescine transport. Both, the uptake induction as well as its specific feedback repression by increased levels of endogenous putrescine requires protein synthesis since they were abolished after addition of cycloheximide for several hours. Our results seem to indicate that putrescine transporter is a stable and specific protein which can be reversibly inactivated by a relatively unstable repressor. Fil:Gonzalez, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cycloheximide
eflornithine
polyamine
putrescine
active transport
animal
article
drug effect
feedback system
growth, development and aging
kinetics
Leishmania mexicana
metabolism
Animals
Biological Transport, Active
Cycloheximide
Eflornithine
Feedback
Kinetics
Leishmania mexicana
Polyamines
Putrescine
spellingShingle cycloheximide
eflornithine
polyamine
putrescine
active transport
animal
article
drug effect
feedback system
growth, development and aging
kinetics
Leishmania mexicana
metabolism
Animals
Biological Transport, Active
Cycloheximide
Eflornithine
Feedback
Kinetics
Leishmania mexicana
Polyamines
Putrescine
Gonzalez, N.S.
Algranati, I.D.
Regulation of putrescine uptake in Leishmania mexicana promastigotes.
topic_facet cycloheximide
eflornithine
polyamine
putrescine
active transport
animal
article
drug effect
feedback system
growth, development and aging
kinetics
Leishmania mexicana
metabolism
Animals
Biological Transport, Active
Cycloheximide
Eflornithine
Feedback
Kinetics
Leishmania mexicana
Polyamines
Putrescine
description Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was strongly repressed by exposure of Leishmania cultures to exogenous putrescine or its derivative 1,4-dimethylputrescine. In contrast, spermidine, spermine, diaminopropane and cadaverine were unable to decrease putrescine transport. Both, the uptake induction as well as its specific feedback repression by increased levels of endogenous putrescine requires protein synthesis since they were abolished after addition of cycloheximide for several hours. Our results seem to indicate that putrescine transporter is a stable and specific protein which can be reversibly inactivated by a relatively unstable repressor.
format JOUR
author Gonzalez, N.S.
Algranati, I.D.
author_facet Gonzalez, N.S.
Algranati, I.D.
author_sort Gonzalez, N.S.
title Regulation of putrescine uptake in Leishmania mexicana promastigotes.
title_short Regulation of putrescine uptake in Leishmania mexicana promastigotes.
title_full Regulation of putrescine uptake in Leishmania mexicana promastigotes.
title_fullStr Regulation of putrescine uptake in Leishmania mexicana promastigotes.
title_full_unstemmed Regulation of putrescine uptake in Leishmania mexicana promastigotes.
title_sort regulation of putrescine uptake in leishmania mexicana promastigotes.
url http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p907_Gonzalez
work_keys_str_mv AT gonzalezns regulationofputrescineuptakeinleishmaniamexicanapromastigotes
AT algranatiid regulationofputrescineuptakeinleishmaniamexicanapromastigotes
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