The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.

Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod R...

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Detalles Bibliográficos
Autores principales: Semino, C.E., Dankert, M.A.
Formato: JOUR
Materias:
lipid linked oligosaccharides
lipid-linked oligosaccharides
lipopolysaccharide
alfalfa
article
bacterial gene
biosynthesis
carbohydrate analysis
chemical structure
chemistry
genetics
metabolism
microbiology
molecular genetics
Sinorhizobium meliloti
Carbohydrate Sequence
Genes, Bacterial
Lipopolysaccharides
Medicago sativa
Molecular Sequence Data
Molecular Structure
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01455680_v40_n7_p1029_Semino
record_format dspace
spelling todo:paper_01455680_v40_n7_p1029_Semino2023-10-03T14:59:54Z The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. Semino, C.E. Dankert, M.A. lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro. Fil:Semino, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic lipid linked oligosaccharides
lipid-linked oligosaccharides
lipopolysaccharide
alfalfa
article
bacterial gene
biosynthesis
carbohydrate analysis
chemical structure
chemistry
genetics
metabolism
microbiology
molecular genetics
Sinorhizobium meliloti
Carbohydrate Sequence
Genes, Bacterial
Lipopolysaccharides
Medicago sativa
Molecular Sequence Data
Molecular Structure
Sinorhizobium meliloti
spellingShingle lipid linked oligosaccharides
lipid-linked oligosaccharides
lipopolysaccharide
alfalfa
article
bacterial gene
biosynthesis
carbohydrate analysis
chemical structure
chemistry
genetics
metabolism
microbiology
molecular genetics
Sinorhizobium meliloti
Carbohydrate Sequence
Genes, Bacterial
Lipopolysaccharides
Medicago sativa
Molecular Sequence Data
Molecular Structure
Sinorhizobium meliloti
Semino, C.E.
Dankert, M.A.
The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
topic_facet lipid linked oligosaccharides
lipid-linked oligosaccharides
lipopolysaccharide
alfalfa
article
bacterial gene
biosynthesis
carbohydrate analysis
chemical structure
chemistry
genetics
metabolism
microbiology
molecular genetics
Sinorhizobium meliloti
Carbohydrate Sequence
Genes, Bacterial
Lipopolysaccharides
Medicago sativa
Molecular Sequence Data
Molecular Structure
Sinorhizobium meliloti
description Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro.
format JOUR
author Semino, C.E.
Dankert, M.A.
author_facet Semino, C.E.
Dankert, M.A.
author_sort Semino, C.E.
title The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_short The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_full The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_fullStr The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_full_unstemmed The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_sort in vitro biosynthesis of functional nodulation factors (nod rm) produced by rhizobium meliloti 1021.
url http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
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AT dankertma theinvitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021
AT seminoce invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021
AT dankertma invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021
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