The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP a...
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todo:paper_0145479X_v47_n5_p383_Chimeo2023-10-03T14:59:54Z The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) Chimeo, C. Fernandez-Gimenez, A.V. Campanella, M. Mendez-Romero, O. Muhlia-Almazan, A. Hypoxia IF1, FoF1-ATPase Inhibitor Shrimp Transcriptional regulation adenosine triphosphatase complementary DNA fibrinogen inhibitory factor 1 messenger RNA transcriptome unclassified drug arthropod protein ATPase inhibitory protein protein proton transporting adenosine triphosphatase adult amino acid sequence animal tissue Article controlled study DNA sequence enzyme activity gene expression gene sequence genetic variability Litopenaeus vannamei marine species mitochondrion nonhuman nucleotide sequence oxygen tension phylogeny reoxygenation RNA sequence sequence homology animal genetics metabolism molecular genetics Penaeidae Animals Arthropod Proteins Base Sequence Molecular Sequence Data Penaeidae Proteins Proton-Translocating ATPases The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension. © 2015, Springer Science+Business Media New York. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0145479X_v47_n5_p383_Chimeo |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Hypoxia IF1, FoF1-ATPase Inhibitor Shrimp Transcriptional regulation adenosine triphosphatase complementary DNA fibrinogen inhibitory factor 1 messenger RNA transcriptome unclassified drug arthropod protein ATPase inhibitory protein protein proton transporting adenosine triphosphatase adult amino acid sequence animal tissue Article controlled study DNA sequence enzyme activity gene expression gene sequence genetic variability Litopenaeus vannamei marine species mitochondrion nonhuman nucleotide sequence oxygen tension phylogeny reoxygenation RNA sequence sequence homology animal genetics metabolism molecular genetics Penaeidae Animals Arthropod Proteins Base Sequence Molecular Sequence Data Penaeidae Proteins Proton-Translocating ATPases |
| spellingShingle |
Hypoxia IF1, FoF1-ATPase Inhibitor Shrimp Transcriptional regulation adenosine triphosphatase complementary DNA fibrinogen inhibitory factor 1 messenger RNA transcriptome unclassified drug arthropod protein ATPase inhibitory protein protein proton transporting adenosine triphosphatase adult amino acid sequence animal tissue Article controlled study DNA sequence enzyme activity gene expression gene sequence genetic variability Litopenaeus vannamei marine species mitochondrion nonhuman nucleotide sequence oxygen tension phylogeny reoxygenation RNA sequence sequence homology animal genetics metabolism molecular genetics Penaeidae Animals Arthropod Proteins Base Sequence Molecular Sequence Data Penaeidae Proteins Proton-Translocating ATPases Chimeo, C. Fernandez-Gimenez, A.V. Campanella, M. Mendez-Romero, O. Muhlia-Almazan, A. The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| topic_facet |
Hypoxia IF1, FoF1-ATPase Inhibitor Shrimp Transcriptional regulation adenosine triphosphatase complementary DNA fibrinogen inhibitory factor 1 messenger RNA transcriptome unclassified drug arthropod protein ATPase inhibitory protein protein proton transporting adenosine triphosphatase adult amino acid sequence animal tissue Article controlled study DNA sequence enzyme activity gene expression gene sequence genetic variability Litopenaeus vannamei marine species mitochondrion nonhuman nucleotide sequence oxygen tension phylogeny reoxygenation RNA sequence sequence homology animal genetics metabolism molecular genetics Penaeidae Animals Arthropod Proteins Base Sequence Molecular Sequence Data Penaeidae Proteins Proton-Translocating ATPases |
| description |
The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension. © 2015, Springer Science+Business Media New York. |
| format |
JOUR |
| author |
Chimeo, C. Fernandez-Gimenez, A.V. Campanella, M. Mendez-Romero, O. Muhlia-Almazan, A. |
| author_facet |
Chimeo, C. Fernandez-Gimenez, A.V. Campanella, M. Mendez-Romero, O. Muhlia-Almazan, A. |
| author_sort |
Chimeo, C. |
| title |
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| title_short |
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| title_full |
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| title_fullStr |
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| title_full_unstemmed |
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1) |
| title_sort |
shrimp mitochondrial fof1-atpase inhibitory factor 1 (if1) |
| url |
http://hdl.handle.net/20.500.12110/paper_0145479X_v47_n5_p383_Chimeo |
| work_keys_str_mv |
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1807323114247290880 |