Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs
The purpose of this study was to investigate in rats, by double-label immunofluorescence and flow cytometric analysis, the age related changes in the CD4 subset of gut-associated lymphoid tissues and spleen. We found that the percentage of CD4 + T cells in Peyer's patches (PP) and spleen (SP) i...
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todo:paper_0145305X_v21_n5_p443_Flo2023-10-03T14:59:53Z Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs Fló, J. Massouh, E. Aging CD4 T cells Gut associated lymphoid tissues Milk Rat cd4 antigen cd45 antigen thy 1 antigen adolescent aged aging animal cell animal tissue article controlled study flow cytometry intestine lymphatic tissue lymphoid organ memory cell mesentery lymph node nonhuman peyer patch priority journal rat spleen t lymphocyte subpopulation Aging Animals B-Lymphocytes CD4-Positive T-Lymphocytes Immunologic Memory Lymphoid Tissue Rats Rats, Wistar T-Lymphocyte Subsets Weaning The purpose of this study was to investigate in rats, by double-label immunofluorescence and flow cytometric analysis, the age related changes in the CD4 subset of gut-associated lymphoid tissues and spleen. We found that the percentage of CD4 + T cells in Peyer's patches (PP) and spleen (SP) increased during the first 6 weeks after weaning. An age-related decrease of the CD4 subset was observed in SP of aged rats, but not in their PP. In all lymphoid tissues studied, an age-related decrease of the Thy-1 + subset was observed from weaning to 2 years of age. Analysis of the naive CD4 subset (CD45RC +) showed that in SP this subset increased during the first 9 weeks of age, and declined in aged rats. However, in PP this subset presented a slow decrease from weaning until 2 years of age. Together with the decrease of the naive subset, a sharp increase of the memory/activated CD4+cells (CD45RC - Thy-1-) was observed in PP, and to a lesser extent in SP. When the maturation of the CD4 T cells in PP was followed during the first week after weaning, we found that an important proportion of this subset changes its phenotype at this time, from recent thymic emigrant (CD45RC-Thy-1 +) to naive T cell (CD45RC + Thy-1 -) and then to activated/memory cell (CD45RC - Thy-1 - ). Therefore it appeared that CD4 T cells from PP mature faster than SP CD4 T cells, and they are not subject to the deleterious effect of aging. One surprising point was the different kinetics of the CD4 T cells observed in mesenteric lymph nodes (MLN). No age-related changes were observed in the CD4 subset at this site. Furthermore, the percentage of the CD45RC + cells did not decrease in aged rats, and in the first 9 weeks of life an increase of this subset was observed. Fil:Massouh, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0145305X_v21_n5_p443_Flo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aging CD4 T cells Gut associated lymphoid tissues Milk Rat cd4 antigen cd45 antigen thy 1 antigen adolescent aged aging animal cell animal tissue article controlled study flow cytometry intestine lymphatic tissue lymphoid organ memory cell mesentery lymph node nonhuman peyer patch priority journal rat spleen t lymphocyte subpopulation Aging Animals B-Lymphocytes CD4-Positive T-Lymphocytes Immunologic Memory Lymphoid Tissue Rats Rats, Wistar T-Lymphocyte Subsets Weaning |
| spellingShingle |
Aging CD4 T cells Gut associated lymphoid tissues Milk Rat cd4 antigen cd45 antigen thy 1 antigen adolescent aged aging animal cell animal tissue article controlled study flow cytometry intestine lymphatic tissue lymphoid organ memory cell mesentery lymph node nonhuman peyer patch priority journal rat spleen t lymphocyte subpopulation Aging Animals B-Lymphocytes CD4-Positive T-Lymphocytes Immunologic Memory Lymphoid Tissue Rats Rats, Wistar T-Lymphocyte Subsets Weaning Fló, J. Massouh, E. Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| topic_facet |
Aging CD4 T cells Gut associated lymphoid tissues Milk Rat cd4 antigen cd45 antigen thy 1 antigen adolescent aged aging animal cell animal tissue article controlled study flow cytometry intestine lymphatic tissue lymphoid organ memory cell mesentery lymph node nonhuman peyer patch priority journal rat spleen t lymphocyte subpopulation Aging Animals B-Lymphocytes CD4-Positive T-Lymphocytes Immunologic Memory Lymphoid Tissue Rats Rats, Wistar T-Lymphocyte Subsets Weaning |
| description |
The purpose of this study was to investigate in rats, by double-label immunofluorescence and flow cytometric analysis, the age related changes in the CD4 subset of gut-associated lymphoid tissues and spleen. We found that the percentage of CD4 + T cells in Peyer's patches (PP) and spleen (SP) increased during the first 6 weeks after weaning. An age-related decrease of the CD4 subset was observed in SP of aged rats, but not in their PP. In all lymphoid tissues studied, an age-related decrease of the Thy-1 + subset was observed from weaning to 2 years of age. Analysis of the naive CD4 subset (CD45RC +) showed that in SP this subset increased during the first 9 weeks of age, and declined in aged rats. However, in PP this subset presented a slow decrease from weaning until 2 years of age. Together with the decrease of the naive subset, a sharp increase of the memory/activated CD4+cells (CD45RC - Thy-1-) was observed in PP, and to a lesser extent in SP. When the maturation of the CD4 T cells in PP was followed during the first week after weaning, we found that an important proportion of this subset changes its phenotype at this time, from recent thymic emigrant (CD45RC-Thy-1 +) to naive T cell (CD45RC + Thy-1 -) and then to activated/memory cell (CD45RC - Thy-1 - ). Therefore it appeared that CD4 T cells from PP mature faster than SP CD4 T cells, and they are not subject to the deleterious effect of aging. One surprising point was the different kinetics of the CD4 T cells observed in mesenteric lymph nodes (MLN). No age-related changes were observed in the CD4 subset at this site. Furthermore, the percentage of the CD45RC + cells did not decrease in aged rats, and in the first 9 weeks of life an increase of this subset was observed. |
| format |
JOUR |
| author |
Fló, J. Massouh, E. |
| author_facet |
Fló, J. Massouh, E. |
| author_sort |
Fló, J. |
| title |
Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| title_short |
Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| title_full |
Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| title_fullStr |
Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| title_full_unstemmed |
Age-related changes of naive and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| title_sort |
age-related changes of naive and memory cd4 rat lymphocyte subsets in mucosal and systemic lymphold organs |
| url |
http://hdl.handle.net/20.500.12110/paper_0145305X_v21_n5_p443_Flo |
| work_keys_str_mv |
AT floj agerelatedchangesofnaiveandmemorycd4ratlymphocytesubsetsinmucosalandsystemiclympholdorgans AT massouhe agerelatedchangesofnaiveandmemorycd4ratlymphocytesubsetsinmucosalandsystemiclympholdorgans |
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1807315266813558784 |