Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea
Young shoots of Phyllostachys aurea showed glucuronoarabinoxylans (GAX) as the major hemicellulosic components, being extracted in major amounts with 1 M KOH (ratio Xyl:Ara:GlcA, 100:67:8), but also with water, showing a broad structural variability. Mixed linkage glucans were also present, but in m...
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todo:paper_01448617_v167_n_p240_Zelaya2023-10-03T14:59:38Z Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea Zelaya, V.M. Fernández, P.V. Vega, A.S. Mantese, A.I. Federico, A.A. Ciancia, M. Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acid determination Bamboo Carboxylic acids Chemical analysis Chemical reactions Extraction Hydroxyproline Proteins Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acids Polymers Young shoots of Phyllostachys aurea showed glucuronoarabinoxylans (GAX) as the major hemicellulosic components, being extracted in major amounts with 1 M KOH (ratio Xyl:Ara:GlcA, 100:67:8), but also with water, showing a broad structural variability. Mixed linkage glucans were also present, but in minor amounts, mostly concentrated in the 4 M KOH extracts, while pectin polymers were very scarce. Arabinogalactan proteins were an important part of water extracts, determined by the presence of the typical arabinogalactan structures (3- and 6-linked Gal p; terminal and 5-linked Ara f), in addition to small amounts of hydroxyproline (2–3% of total protein) and positive reaction to Yariv's reagent. Morphological and anatomical characteristics of young shoots are described, as well as localization of some cell wall components, and related with chemical analysis. A method for determination of uronic acids as their N-propylaldonamide acetates and separation and quantification by GC/MS was adapted for its use with grass cell wall fractions. © 2017 Elsevier Ltd Fil:Fernández, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vega, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v167_n_p240_Zelaya |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acid determination Bamboo Carboxylic acids Chemical analysis Chemical reactions Extraction Hydroxyproline Proteins Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acids Polymers |
| spellingShingle |
Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acid determination Bamboo Carboxylic acids Chemical analysis Chemical reactions Extraction Hydroxyproline Proteins Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acids Polymers Zelaya, V.M. Fernández, P.V. Vega, A.S. Mantese, A.I. Federico, A.A. Ciancia, M. Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| topic_facet |
Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acid determination Bamboo Carboxylic acids Chemical analysis Chemical reactions Extraction Hydroxyproline Proteins Arabinogalactan Bamboo shoots Glucuronoarabinoxylan Mixed linkage glucan Phyllostachys aurea Uronic acids Polymers |
| description |
Young shoots of Phyllostachys aurea showed glucuronoarabinoxylans (GAX) as the major hemicellulosic components, being extracted in major amounts with 1 M KOH (ratio Xyl:Ara:GlcA, 100:67:8), but also with water, showing a broad structural variability. Mixed linkage glucans were also present, but in minor amounts, mostly concentrated in the 4 M KOH extracts, while pectin polymers were very scarce. Arabinogalactan proteins were an important part of water extracts, determined by the presence of the typical arabinogalactan structures (3- and 6-linked Gal p; terminal and 5-linked Ara f), in addition to small amounts of hydroxyproline (2–3% of total protein) and positive reaction to Yariv's reagent. Morphological and anatomical characteristics of young shoots are described, as well as localization of some cell wall components, and related with chemical analysis. A method for determination of uronic acids as their N-propylaldonamide acetates and separation and quantification by GC/MS was adapted for its use with grass cell wall fractions. © 2017 Elsevier Ltd |
| format |
JOUR |
| author |
Zelaya, V.M. Fernández, P.V. Vega, A.S. Mantese, A.I. Federico, A.A. Ciancia, M. |
| author_facet |
Zelaya, V.M. Fernández, P.V. Vega, A.S. Mantese, A.I. Federico, A.A. Ciancia, M. |
| author_sort |
Zelaya, V.M. |
| title |
Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| title_short |
Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| title_full |
Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| title_fullStr |
Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| title_full_unstemmed |
Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea |
| title_sort |
glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo phyllostachys aurea |
| url |
http://hdl.handle.net/20.500.12110/paper_01448617_v167_n_p240_Zelaya |
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