Chiroptical characterization of homopolymeric block fractions in alginates
Homopolymannuronic and homopolyguluronic fractions were obtained by partial hydrolysis of the alkaline extracts from the brown seaweeds Ascoseira mirabilis, Desmarestia menziessi, Desmarestia ligulata and Durvillaea sp. collected in southern Chile. Full characterization of the fractions was achieved...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01448617_v146_n_p90_MartinezGomez |
| Aporte de: |
| id |
todo:paper_01448617_v146_n_p90_MartinezGomez |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01448617_v146_n_p90_MartinezGomez2023-10-03T14:59:36Z Chiroptical characterization of homopolymeric block fractions in alginates Martínez-Gómez, F. Mansilla, A. Matsuhiro, B. Matulewicz, M.C. Troncoso-Valenzuela, M.A. Alginate Circular dichroism Homopolyguluronic acid Homopolymannuronic acid Optical rotatory dispersion Specific optical rotation Alginate Dichroism Hydrolysis Monomers Nuclear magnetic resonance spectroscopy Absolute configuration Ft-ir and nmr spectroscopies Guluronic acid Mannuronic acids Negative values Optical rotatory dispersion Partial hydrolysis Specific optical rotations Optical rotation alginic acid polymer brown alga chemistry infrared spectroscopy nuclear magnetic resonance spectroscopy Alginates Magnetic Resonance Spectroscopy Phaeophyta Polymers Spectroscopy, Fourier Transform Infrared Homopolymannuronic and homopolyguluronic fractions were obtained by partial hydrolysis of the alkaline extracts from the brown seaweeds Ascoseira mirabilis, Desmarestia menziessi, Desmarestia ligulata and Durvillaea sp. collected in southern Chile. Full characterization of the fractions was achieved by FT-IR and NMR spectroscopy. Total hydrolysis with 90% formic acid of the homopolymeric fractions allowed the preparation of mannuronic and guluronic acids. Both monomers and homopolymeric fractions as neutral salts were studied by CD and ORD. Chiroptical spectra were similar in shape and sign to those previously published in the literature, and permitted to assign D configuration to mannuronic acid and L configuration to guluronic acid in alginic acids. Specific optical rotation values at the sodium D light for the homopolymannuronic (∼-100°) and homopolyguluronic (∼-110°) acid fractions were obtained. These high negative values are proposed for the assignment of the absolute configuration of monomers in homopolymeric fractions. © 2016 Elsevier Ltd. All rights reserved. Fil:Matsuhiro, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v146_n_p90_MartinezGomez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alginate Circular dichroism Homopolyguluronic acid Homopolymannuronic acid Optical rotatory dispersion Specific optical rotation Alginate Dichroism Hydrolysis Monomers Nuclear magnetic resonance spectroscopy Absolute configuration Ft-ir and nmr spectroscopies Guluronic acid Mannuronic acids Negative values Optical rotatory dispersion Partial hydrolysis Specific optical rotations Optical rotation alginic acid polymer brown alga chemistry infrared spectroscopy nuclear magnetic resonance spectroscopy Alginates Magnetic Resonance Spectroscopy Phaeophyta Polymers Spectroscopy, Fourier Transform Infrared |
| spellingShingle |
Alginate Circular dichroism Homopolyguluronic acid Homopolymannuronic acid Optical rotatory dispersion Specific optical rotation Alginate Dichroism Hydrolysis Monomers Nuclear magnetic resonance spectroscopy Absolute configuration Ft-ir and nmr spectroscopies Guluronic acid Mannuronic acids Negative values Optical rotatory dispersion Partial hydrolysis Specific optical rotations Optical rotation alginic acid polymer brown alga chemistry infrared spectroscopy nuclear magnetic resonance spectroscopy Alginates Magnetic Resonance Spectroscopy Phaeophyta Polymers Spectroscopy, Fourier Transform Infrared Martínez-Gómez, F. Mansilla, A. Matsuhiro, B. Matulewicz, M.C. Troncoso-Valenzuela, M.A. Chiroptical characterization of homopolymeric block fractions in alginates |
| topic_facet |
Alginate Circular dichroism Homopolyguluronic acid Homopolymannuronic acid Optical rotatory dispersion Specific optical rotation Alginate Dichroism Hydrolysis Monomers Nuclear magnetic resonance spectroscopy Absolute configuration Ft-ir and nmr spectroscopies Guluronic acid Mannuronic acids Negative values Optical rotatory dispersion Partial hydrolysis Specific optical rotations Optical rotation alginic acid polymer brown alga chemistry infrared spectroscopy nuclear magnetic resonance spectroscopy Alginates Magnetic Resonance Spectroscopy Phaeophyta Polymers Spectroscopy, Fourier Transform Infrared |
| description |
Homopolymannuronic and homopolyguluronic fractions were obtained by partial hydrolysis of the alkaline extracts from the brown seaweeds Ascoseira mirabilis, Desmarestia menziessi, Desmarestia ligulata and Durvillaea sp. collected in southern Chile. Full characterization of the fractions was achieved by FT-IR and NMR spectroscopy. Total hydrolysis with 90% formic acid of the homopolymeric fractions allowed the preparation of mannuronic and guluronic acids. Both monomers and homopolymeric fractions as neutral salts were studied by CD and ORD. Chiroptical spectra were similar in shape and sign to those previously published in the literature, and permitted to assign D configuration to mannuronic acid and L configuration to guluronic acid in alginic acids. Specific optical rotation values at the sodium D light for the homopolymannuronic (∼-100°) and homopolyguluronic (∼-110°) acid fractions were obtained. These high negative values are proposed for the assignment of the absolute configuration of monomers in homopolymeric fractions. © 2016 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Martínez-Gómez, F. Mansilla, A. Matsuhiro, B. Matulewicz, M.C. Troncoso-Valenzuela, M.A. |
| author_facet |
Martínez-Gómez, F. Mansilla, A. Matsuhiro, B. Matulewicz, M.C. Troncoso-Valenzuela, M.A. |
| author_sort |
Martínez-Gómez, F. |
| title |
Chiroptical characterization of homopolymeric block fractions in alginates |
| title_short |
Chiroptical characterization of homopolymeric block fractions in alginates |
| title_full |
Chiroptical characterization of homopolymeric block fractions in alginates |
| title_fullStr |
Chiroptical characterization of homopolymeric block fractions in alginates |
| title_full_unstemmed |
Chiroptical characterization of homopolymeric block fractions in alginates |
| title_sort |
chiroptical characterization of homopolymeric block fractions in alginates |
| url |
http://hdl.handle.net/20.500.12110/paper_01448617_v146_n_p90_MartinezGomez |
| work_keys_str_mv |
AT martinezgomezf chiropticalcharacterizationofhomopolymericblockfractionsinalginates AT mansillaa chiropticalcharacterizationofhomopolymericblockfractionsinalginates AT matsuhirob chiropticalcharacterizationofhomopolymericblockfractionsinalginates AT matulewiczmc chiropticalcharacterizationofhomopolymericblockfractionsinalginates AT troncosovalenzuelama chiropticalcharacterizationofhomopolymericblockfractionsinalginates |
| _version_ |
1807321031982972928 |