The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity
Room-temperature acid (pH 2) extraction of Dictyota dichotoma thalli yielded 2.2% of sulfated polysaccharides. Further extraction with the same solvent at 70 C was conducted sequentially for nine times, with a total yield of 7.2%. Fucose was the main monosaccharide only in the room-temperature extra...
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todo:paper_01448617_v101_n1_p804_Rabanal2023-10-03T14:59:31Z The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity Rabanal, M. Ponce, N.M.A. Navarro, D.A. Gómez, R.M. Stortz, C.A. Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Brown seaweed Dichotoma Fucoidans Galactofucans Extraction Glucose Polysaccharides Seaweed Sodium chloride Viruses Carboxylic acids Coxsackievirus Dictyota dichotoma Herpesviridae Human herpesvirus 1 antivirus agent fucoidin polysaccharide article brown alga Brown seaweeds chemical phenomena chemistry Dictyota dichotoma drug effect Enterovirus Fucoidans Galactofucans Herpes simplex virus 1 IC 50 pH temperature Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Agents Enterovirus Herpesvirus 1, Human Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Inhibitory Concentration 50 Phaeophyta Polysaccharides Temperature Room-temperature acid (pH 2) extraction of Dictyota dichotoma thalli yielded 2.2% of sulfated polysaccharides. Further extraction with the same solvent at 70 C was conducted sequentially for nine times, with a total yield of 7.2%. Fucose was the main monosaccharide only in the room-temperature extract (EAR) and in the first 70 C extract (EAH1). The remaining fractions showed increasing amounts of mannose (the main neutral monosaccharide), xylose and uronic acids. Fractionation by means of cetrimide precipitation and redissolution in increasing sodium chloride solutions has allowed obtaining several subfractions from each extract. The fractions redissolved at lower NaCl concentrations have large amounts of uronic acids and lesser sulfate contents, whereas those redissolved at higher NaCl concentrations are heavily sulfated and have low uronic acid contents. For the fucose-rich extracts (EAR and EAH1), fractionation leads to uronoxylomannofucan-rich and galactofucan-rich fractions. The remaining extracts gave rise to complex mixtures, with mannose and uronic acid-rich polysaccharides. Moderate inhibitory effect against herpes virus (HSV-1) and Coxsackie virus (CVB3) were found for the galactofucan-rich fractions. Most of the other fractions were inactive against both viruses, although some xylomannan-rich fractions were also active against HSV-1. © 2013 Elsevier Ltd. All rights reserved. Fil:Navarro, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v101_n1_p804_Rabanal |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Brown seaweed Dichotoma Fucoidans Galactofucans Extraction Glucose Polysaccharides Seaweed Sodium chloride Viruses Carboxylic acids Coxsackievirus Dictyota dichotoma Herpesviridae Human herpesvirus 1 antivirus agent fucoidin polysaccharide article brown alga Brown seaweeds chemical phenomena chemistry Dictyota dichotoma drug effect Enterovirus Fucoidans Galactofucans Herpes simplex virus 1 IC 50 pH temperature Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Agents Enterovirus Herpesvirus 1, Human Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Inhibitory Concentration 50 Phaeophyta Polysaccharides Temperature |
| spellingShingle |
Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Brown seaweed Dichotoma Fucoidans Galactofucans Extraction Glucose Polysaccharides Seaweed Sodium chloride Viruses Carboxylic acids Coxsackievirus Dictyota dichotoma Herpesviridae Human herpesvirus 1 antivirus agent fucoidin polysaccharide article brown alga Brown seaweeds chemical phenomena chemistry Dictyota dichotoma drug effect Enterovirus Fucoidans Galactofucans Herpes simplex virus 1 IC 50 pH temperature Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Agents Enterovirus Herpesvirus 1, Human Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Inhibitory Concentration 50 Phaeophyta Polysaccharides Temperature Rabanal, M. Ponce, N.M.A. Navarro, D.A. Gómez, R.M. Stortz, C.A. The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| topic_facet |
Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Brown seaweed Dichotoma Fucoidans Galactofucans Extraction Glucose Polysaccharides Seaweed Sodium chloride Viruses Carboxylic acids Coxsackievirus Dictyota dichotoma Herpesviridae Human herpesvirus 1 antivirus agent fucoidin polysaccharide article brown alga Brown seaweeds chemical phenomena chemistry Dictyota dichotoma drug effect Enterovirus Fucoidans Galactofucans Herpes simplex virus 1 IC 50 pH temperature Antiviral Brown seaweeds Dictyota dichotoma Fucoidans Galactofucans Antiviral Agents Enterovirus Herpesvirus 1, Human Hydrogen-Ion Concentration Hydrophobic and Hydrophilic Interactions Inhibitory Concentration 50 Phaeophyta Polysaccharides Temperature |
| description |
Room-temperature acid (pH 2) extraction of Dictyota dichotoma thalli yielded 2.2% of sulfated polysaccharides. Further extraction with the same solvent at 70 C was conducted sequentially for nine times, with a total yield of 7.2%. Fucose was the main monosaccharide only in the room-temperature extract (EAR) and in the first 70 C extract (EAH1). The remaining fractions showed increasing amounts of mannose (the main neutral monosaccharide), xylose and uronic acids. Fractionation by means of cetrimide precipitation and redissolution in increasing sodium chloride solutions has allowed obtaining several subfractions from each extract. The fractions redissolved at lower NaCl concentrations have large amounts of uronic acids and lesser sulfate contents, whereas those redissolved at higher NaCl concentrations are heavily sulfated and have low uronic acid contents. For the fucose-rich extracts (EAR and EAH1), fractionation leads to uronoxylomannofucan-rich and galactofucan-rich fractions. The remaining extracts gave rise to complex mixtures, with mannose and uronic acid-rich polysaccharides. Moderate inhibitory effect against herpes virus (HSV-1) and Coxsackie virus (CVB3) were found for the galactofucan-rich fractions. Most of the other fractions were inactive against both viruses, although some xylomannan-rich fractions were also active against HSV-1. © 2013 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Rabanal, M. Ponce, N.M.A. Navarro, D.A. Gómez, R.M. Stortz, C.A. |
| author_facet |
Rabanal, M. Ponce, N.M.A. Navarro, D.A. Gómez, R.M. Stortz, C.A. |
| author_sort |
Rabanal, M. |
| title |
The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| title_short |
The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| title_full |
The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| title_fullStr |
The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| title_full_unstemmed |
The system of fucoidans from the brown seaweed Dictyota dichotoma: Chemical analysis and antiviral activity |
| title_sort |
system of fucoidans from the brown seaweed dictyota dichotoma: chemical analysis and antiviral activity |
| url |
http://hdl.handle.net/20.500.12110/paper_01448617_v101_n1_p804_Rabanal |
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