Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs

Many intracellular Ca2+ signals involve Ca2+ release from the endoplasmic reticulum through inositol 1,4,5-trisphosphate receptors (IP3Rs). The open probability of IP3Rs depends on cytosolic Ca2+ so that these signals involve Ca2+-induced Ca2+-release (CICR). IP3Rs are organized in clusters. The sig...

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Autores principales:	Piegari, E., Sigaut, L., Ponce Dawson, S.
Formato:	JOUR
Materias:	Calcium signals CICR in clusters Dyes Puffs calcium ion dye egtazic acid inositol 1,4,5 trisphosphate receptor aniline derivative calcium Fluo 4 inositol 1,4,5 trisphosphate xanthene derivative animal cell Article calcium cell level calcium puff calcium signaling calcium transport chemical reaction kinetics female fluorescence mathematical analysis nonhuman simulation algorithm animal chemistry confocal microscopy drug effects growth, development and aging kinetics metabolism oocyte Xenopus Algorithms Aniline Compounds Animals Calcium Calcium Signaling Egtazic Acid Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Kinetics Microscopy, Confocal Oocytes Xanthenes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01434160_v57_n2_p109_Piegari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01434160_v57_n2_p109_Piegari
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spelling	todo:paper_01434160_v57_n2_p109_Piegari2023-10-03T14:59:22Z Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs Piegari, E. Sigaut, L. Ponce Dawson, S. Calcium signals CICR in clusters Dyes Puffs calcium ion dye egtazic acid inositol 1,4,5 trisphosphate receptor aniline derivative calcium egtazic acid Fluo 4 inositol 1,4,5 trisphosphate inositol 1,4,5 trisphosphate receptor xanthene derivative animal cell Article calcium cell level calcium puff calcium signaling calcium transport chemical reaction kinetics female fluorescence mathematical analysis nonhuman simulation algorithm animal chemistry confocal microscopy drug effects growth, development and aging kinetics metabolism oocyte Xenopus Algorithms Aniline Compounds Animals Calcium Calcium Signaling Egtazic Acid Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Kinetics Microscopy, Confocal Oocytes Xanthenes Xenopus Many intracellular Ca2+ signals involve Ca2+ release from the endoplasmic reticulum through inositol 1,4,5-trisphosphate receptors (IP3Rs). The open probability of IP3Rs depends on cytosolic Ca2+ so that these signals involve Ca2+-induced Ca2+-release (CICR). IP3Rs are organized in clusters. The signals they mediate are observed using single-wavelength dyes and, often, a slow Ca2+ buffer (EGTA) is added to disrupt CICR between clusters and keep the signals spatially restricted. It is assumed that the presence of the dye or of EGTA does not alter the intra-cluster Ca2+ dynamics. In this paper we analyze this issue combining experiments and numerical simulations. We compare the properties of local signals known as puffs observed with different dyes and EGTA concentrations. We determine that although the dye or EGTA does not alter the intra-cluster dynamics, the set of observable events is different depending on the degree of inter-cluster uncoupling of the experiment. An analysis of the observations shows that the events that are missed for insufficient inter-cluster uncoupling are those of fastest amplitude growth rate. This agrees with a spatial organization in which the largest amplitude events correspond to clusters with densely packed active IP3Rs. © 2015 Elsevier Ltd. Fil:Sigaut, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ponce Dawson, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01434160_v57_n2_p109_Piegari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium signals CICR in clusters Dyes Puffs calcium ion dye egtazic acid inositol 1,4,5 trisphosphate receptor aniline derivative calcium egtazic acid Fluo 4 inositol 1,4,5 trisphosphate inositol 1,4,5 trisphosphate receptor xanthene derivative animal cell Article calcium cell level calcium puff calcium signaling calcium transport chemical reaction kinetics female fluorescence mathematical analysis nonhuman simulation algorithm animal chemistry confocal microscopy drug effects growth, development and aging kinetics metabolism oocyte Xenopus Algorithms Aniline Compounds Animals Calcium Calcium Signaling Egtazic Acid Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Kinetics Microscopy, Confocal Oocytes Xanthenes Xenopus
spellingShingle	Calcium signals CICR in clusters Dyes Puffs calcium ion dye egtazic acid inositol 1,4,5 trisphosphate receptor aniline derivative calcium egtazic acid Fluo 4 inositol 1,4,5 trisphosphate inositol 1,4,5 trisphosphate receptor xanthene derivative animal cell Article calcium cell level calcium puff calcium signaling calcium transport chemical reaction kinetics female fluorescence mathematical analysis nonhuman simulation algorithm animal chemistry confocal microscopy drug effects growth, development and aging kinetics metabolism oocyte Xenopus Algorithms Aniline Compounds Animals Calcium Calcium Signaling Egtazic Acid Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Kinetics Microscopy, Confocal Oocytes Xanthenes Xenopus Piegari, E. Sigaut, L. Ponce Dawson, S. Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
topic_facet	Calcium signals CICR in clusters Dyes Puffs calcium ion dye egtazic acid inositol 1,4,5 trisphosphate receptor aniline derivative calcium egtazic acid Fluo 4 inositol 1,4,5 trisphosphate inositol 1,4,5 trisphosphate receptor xanthene derivative animal cell Article calcium cell level calcium puff calcium signaling calcium transport chemical reaction kinetics female fluorescence mathematical analysis nonhuman simulation algorithm animal chemistry confocal microscopy drug effects growth, development and aging kinetics metabolism oocyte Xenopus Algorithms Aniline Compounds Animals Calcium Calcium Signaling Egtazic Acid Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Kinetics Microscopy, Confocal Oocytes Xanthenes Xenopus
description	Many intracellular Ca2+ signals involve Ca2+ release from the endoplasmic reticulum through inositol 1,4,5-trisphosphate receptors (IP3Rs). The open probability of IP3Rs depends on cytosolic Ca2+ so that these signals involve Ca2+-induced Ca2+-release (CICR). IP3Rs are organized in clusters. The signals they mediate are observed using single-wavelength dyes and, often, a slow Ca2+ buffer (EGTA) is added to disrupt CICR between clusters and keep the signals spatially restricted. It is assumed that the presence of the dye or of EGTA does not alter the intra-cluster Ca2+ dynamics. In this paper we analyze this issue combining experiments and numerical simulations. We compare the properties of local signals known as puffs observed with different dyes and EGTA concentrations. We determine that although the dye or EGTA does not alter the intra-cluster dynamics, the set of observable events is different depending on the degree of inter-cluster uncoupling of the experiment. An analysis of the observations shows that the events that are missed for insufficient inter-cluster uncoupling are those of fastest amplitude growth rate. This agrees with a spatial organization in which the largest amplitude events correspond to clusters with densely packed active IP3Rs. © 2015 Elsevier Ltd.
format	JOUR
author	Piegari, E. Sigaut, L. Ponce Dawson, S.
author_facet	Piegari, E. Sigaut, L. Ponce Dawson, S.
author_sort	Piegari, E.
title	Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
title_short	Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
title_full	Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
title_fullStr	Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
title_full_unstemmed	Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs
title_sort	ca2+ images obtained in different experimental conditions shed light on the spatial distribution of ip3 receptors that underlie ca2+ puffs
url	http://hdl.handle.net/20.500.12110/paper_01434160_v57_n2_p109_Piegari
work_keys_str_mv	AT piegarie ca2imagesobtainedindifferentexperimentalconditionsshedlightonthespatialdistributionofip3receptorsthatunderlieca2puffs AT sigautl ca2imagesobtainedindifferentexperimentalconditionsshedlightonthespatialdistributionofip3receptorsthatunderlieca2puffs AT poncedawsons ca2imagesobtainedindifferentexperimentalconditionsshedlightonthespatialdistributionofip3receptorsthatunderlieca2puffs
_version_	1782027713832288256

Ca2+ images obtained in different experimental conditions shed light on the spatial distribution of IP3 receptors that underlie Ca2+ puffs

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