Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters
Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage....
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todo:paper_01410229_v46_n3-4_p281_DaRe2023-10-03T14:58:33Z Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters Da Re, V. Papinutti, L. Forchiassin, F. Levin, L. Biobleaching Laccase Luminance Mn-peroxidase Softwood kraft pulp Trametes trogii Biobleaching Buffer pH Doehlert Experimental design Independent variables Laccase Laccases Loblolly pine Operating condition Peroxide concentration Process parameters Pulp yield loss Reference standard Response Surface Methodology Sodium succinate Softwood kraft pulps Supernatants Totally chlorine-free Treatment time White-rot fungus trametes Whole process Bleaching Chlorine Cleaning Enzymes Hydrogen peroxide Kraft process Manganese Manganese compounds Oxidation pH effects Sodium Softwoods Statistics Titanium Titanium oxides Kraft pulp chlorine hydrogen peroxide laccase peroxidase peroxide succinic acid titanium dioxide article bleaching controlled study culture medium experimental design incubation time loblolly pine luminance nonhuman pH response surface method supernatant temperature time Trametes Trametes trogii white rot fungus wood pulp Biobleaching Bleaching Chlorine Cleaning Enzymes Hydrogen Peroxide Kraft Pulps Laccase Manganese Manganese Compounds Oxidation Sodium Softwood Pulps Softwoods Statistics Titanium Funalia trogii Fungi Pinus taeda Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme. © 2009 Elsevier Inc. All rights reserved. Fil:Papinutti, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01410229_v46_n3-4_p281_DaRe |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biobleaching Laccase Luminance Mn-peroxidase Softwood kraft pulp Trametes trogii Biobleaching Buffer pH Doehlert Experimental design Independent variables Laccase Laccases Loblolly pine Operating condition Peroxide concentration Process parameters Pulp yield loss Reference standard Response Surface Methodology Sodium succinate Softwood kraft pulps Supernatants Totally chlorine-free Treatment time White-rot fungus trametes Whole process Bleaching Chlorine Cleaning Enzymes Hydrogen peroxide Kraft process Manganese Manganese compounds Oxidation pH effects Sodium Softwoods Statistics Titanium Titanium oxides Kraft pulp chlorine hydrogen peroxide laccase peroxidase peroxide succinic acid titanium dioxide article bleaching controlled study culture medium experimental design incubation time loblolly pine luminance nonhuman pH response surface method supernatant temperature time Trametes Trametes trogii white rot fungus wood pulp Biobleaching Bleaching Chlorine Cleaning Enzymes Hydrogen Peroxide Kraft Pulps Laccase Manganese Manganese Compounds Oxidation Sodium Softwood Pulps Softwoods Statistics Titanium Funalia trogii Fungi Pinus taeda |
| spellingShingle |
Biobleaching Laccase Luminance Mn-peroxidase Softwood kraft pulp Trametes trogii Biobleaching Buffer pH Doehlert Experimental design Independent variables Laccase Laccases Loblolly pine Operating condition Peroxide concentration Process parameters Pulp yield loss Reference standard Response Surface Methodology Sodium succinate Softwood kraft pulps Supernatants Totally chlorine-free Treatment time White-rot fungus trametes Whole process Bleaching Chlorine Cleaning Enzymes Hydrogen peroxide Kraft process Manganese Manganese compounds Oxidation pH effects Sodium Softwoods Statistics Titanium Titanium oxides Kraft pulp chlorine hydrogen peroxide laccase peroxidase peroxide succinic acid titanium dioxide article bleaching controlled study culture medium experimental design incubation time loblolly pine luminance nonhuman pH response surface method supernatant temperature time Trametes Trametes trogii white rot fungus wood pulp Biobleaching Bleaching Chlorine Cleaning Enzymes Hydrogen Peroxide Kraft Pulps Laccase Manganese Manganese Compounds Oxidation Sodium Softwood Pulps Softwoods Statistics Titanium Funalia trogii Fungi Pinus taeda Da Re, V. Papinutti, L. Forchiassin, F. Levin, L. Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| topic_facet |
Biobleaching Laccase Luminance Mn-peroxidase Softwood kraft pulp Trametes trogii Biobleaching Buffer pH Doehlert Experimental design Independent variables Laccase Laccases Loblolly pine Operating condition Peroxide concentration Process parameters Pulp yield loss Reference standard Response Surface Methodology Sodium succinate Softwood kraft pulps Supernatants Totally chlorine-free Treatment time White-rot fungus trametes Whole process Bleaching Chlorine Cleaning Enzymes Hydrogen peroxide Kraft process Manganese Manganese compounds Oxidation pH effects Sodium Softwoods Statistics Titanium Titanium oxides Kraft pulp chlorine hydrogen peroxide laccase peroxidase peroxide succinic acid titanium dioxide article bleaching controlled study culture medium experimental design incubation time loblolly pine luminance nonhuman pH response surface method supernatant temperature time Trametes Trametes trogii white rot fungus wood pulp Biobleaching Bleaching Chlorine Cleaning Enzymes Hydrogen Peroxide Kraft Pulps Laccase Manganese Manganese Compounds Oxidation Sodium Softwood Pulps Softwoods Statistics Titanium Funalia trogii Fungi Pinus taeda |
| description |
Loblolly pine kraft pulp was bleached in a totally chlorine-free sequence that involved treatment with culture supernatants from the white-rot fungus Trametes trogii followed by a peroxide stage. The whole process was performed at 28 °C, and did not require mediator addition in the enzymatic stage. Different operating conditions in the peroxide stage (pH, peroxide concentration and treatment time), were tested by using response surface methodology based on a Doehlert experimental design, in order to describe their effects and normalize a biobleaching protocol. The results showed that all three independent variables had significant effect on the luminance (L*) and Chroma (C*) of the enzyme-treated pulp. Best results were obtained after 1 h of enzyme incubation (352 U laccase, 2 U Mn-peroxidase per g of oven-dry pulp), followed by 96 h treatment with 2.5% hydrogen peroxide in sodium succinate buffer pH 6 (5% consistency). We obtained a noteworthy increase in L* = 94.45 (compared with 94.5 of the white reference standard (titanium oxide), 69.94 of the initial pulp, and 83.11 of the peroxide-bleached control), a decrease in C* (9.85), with minor pulp yield loss (less than 5%), under essentially mild conditions, using a low-cost source of enzyme. © 2009 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Da Re, V. Papinutti, L. Forchiassin, F. Levin, L. |
| author_facet |
Da Re, V. Papinutti, L. Forchiassin, F. Levin, L. |
| author_sort |
Da Re, V. |
| title |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| title_short |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| title_full |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| title_fullStr |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| title_full_unstemmed |
Biobleaching of loblolly pine kraft pulp with Trametes trogii culture fluids followed by a peroxide stage. Application of Doehlert experimental design to evaluate process parameters |
| title_sort |
biobleaching of loblolly pine kraft pulp with trametes trogii culture fluids followed by a peroxide stage. application of doehlert experimental design to evaluate process parameters |
| url |
http://hdl.handle.net/20.500.12110/paper_01410229_v46_n3-4_p281_DaRe |
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