The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing

INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments a...

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Autores principales:	Carlotto, N., Wirth, S., Furman, N., Ferreyra Solari, N., Ariel, F., Crespi, M., Kobayashi, K.
Formato:	JOUR
Materias:	Cell-to-cell communication Chloroplast Retrograde signals cells and cell components chloroplast dicotyledon enzyme activity gene expression genetic analysis photosynthesis pigment RNA Arabidopsis Arabidopsis protein chloroplast RNA DEAD box protein INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis RNA helicase drug effects enzymology gene expression regulation genetics intron metabolism mutation plasmodesma reporter gene RNA splicing seedling transgenic plant transport at the cellular level Arabidopsis Proteins Biological Transport Chloroplasts DEAD-box RNA Helicases Gene Expression Regulation, Plant Genes, Reporter Introns Mutation Photosynthesis Plants, Genetically Modified Plasmodesmata RNA Helicases RNA Splicing RNA, Chloroplast Seedlings
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01407791_v39_n1_p165_Carlotto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01407791_v39_n1_p165_Carlotto
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spelling	todo:paper_01407791_v39_n1_p165_Carlotto2023-10-03T14:58:30Z The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing Carlotto, N. Wirth, S. Furman, N. Ferreyra Solari, N. Ariel, F. Crespi, M. Kobayashi, K. Cell-to-cell communication Chloroplast Retrograde signals cells and cell components chloroplast dicotyledon enzyme activity gene expression genetic analysis photosynthesis pigment RNA Arabidopsis Arabidopsis protein chloroplast RNA DEAD box protein INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis RNA helicase Arabidopsis chloroplast drug effects enzymology gene expression regulation genetics intron metabolism mutation photosynthesis plasmodesma reporter gene RNA splicing seedling transgenic plant transport at the cellular level Arabidopsis Arabidopsis Proteins Biological Transport Chloroplasts DEAD-box RNA Helicases Gene Expression Regulation, Plant Genes, Reporter Introns Mutation Photosynthesis Plants, Genetically Modified Plasmodesmata RNA Helicases RNA Splicing RNA, Chloroplast Seedlings INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants. © 2016 John Wiley & Sons Ltd. Fil:Wirth, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Furman, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Crespi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01407791_v39_n1_p165_Carlotto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell-to-cell communication Chloroplast Retrograde signals cells and cell components chloroplast dicotyledon enzyme activity gene expression genetic analysis photosynthesis pigment RNA Arabidopsis Arabidopsis protein chloroplast RNA DEAD box protein INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis RNA helicase Arabidopsis chloroplast drug effects enzymology gene expression regulation genetics intron metabolism mutation photosynthesis plasmodesma reporter gene RNA splicing seedling transgenic plant transport at the cellular level Arabidopsis Arabidopsis Proteins Biological Transport Chloroplasts DEAD-box RNA Helicases Gene Expression Regulation, Plant Genes, Reporter Introns Mutation Photosynthesis Plants, Genetically Modified Plasmodesmata RNA Helicases RNA Splicing RNA, Chloroplast Seedlings
spellingShingle	Cell-to-cell communication Chloroplast Retrograde signals cells and cell components chloroplast dicotyledon enzyme activity gene expression genetic analysis photosynthesis pigment RNA Arabidopsis Arabidopsis protein chloroplast RNA DEAD box protein INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis RNA helicase Arabidopsis chloroplast drug effects enzymology gene expression regulation genetics intron metabolism mutation photosynthesis plasmodesma reporter gene RNA splicing seedling transgenic plant transport at the cellular level Arabidopsis Arabidopsis Proteins Biological Transport Chloroplasts DEAD-box RNA Helicases Gene Expression Regulation, Plant Genes, Reporter Introns Mutation Photosynthesis Plants, Genetically Modified Plasmodesmata RNA Helicases RNA Splicing RNA, Chloroplast Seedlings Carlotto, N. Wirth, S. Furman, N. Ferreyra Solari, N. Ariel, F. Crespi, M. Kobayashi, K. The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
topic_facet	Cell-to-cell communication Chloroplast Retrograde signals cells and cell components chloroplast dicotyledon enzyme activity gene expression genetic analysis photosynthesis pigment RNA Arabidopsis Arabidopsis protein chloroplast RNA DEAD box protein INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis RNA helicase Arabidopsis chloroplast drug effects enzymology gene expression regulation genetics intron metabolism mutation photosynthesis plasmodesma reporter gene RNA splicing seedling transgenic plant transport at the cellular level Arabidopsis Arabidopsis Proteins Biological Transport Chloroplasts DEAD-box RNA Helicases Gene Expression Regulation, Plant Genes, Reporter Introns Mutation Photosynthesis Plants, Genetically Modified Plasmodesmata RNA Helicases RNA Splicing RNA, Chloroplast Seedlings
description	INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants. © 2016 John Wiley & Sons Ltd.
format	JOUR
author	Carlotto, N. Wirth, S. Furman, N. Ferreyra Solari, N. Ariel, F. Crespi, M. Kobayashi, K.
author_facet	Carlotto, N. Wirth, S. Furman, N. Ferreyra Solari, N. Ariel, F. Crespi, M. Kobayashi, K.
author_sort	Carlotto, N.
title	The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
title_short	The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
title_full	The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
title_fullStr	The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
title_full_unstemmed	The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
title_sort	chloroplastic devh-box rna helicase increased size exclusion limit 2 involved in plasmodesmata regulation is required for group ii intron splicing
url	http://hdl.handle.net/20.500.12110/paper_01407791_v39_n1_p165_Carlotto
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The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing

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