A new world monkey microsatellite (AP74) higly conserved in primates

Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in bloo...

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Autores principales:	Oklander, L.I., Steinberg, E.R., Mudry, M.D.
Formato:	JOUR
Materias:	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0120548X_v17_n1_p93_Oklander
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spelling	todo:paper_0120548X_v17_n1_p93_Oklander2023-10-03T14:58:02Z A new world monkey microsatellite (AP74) higly conserved in primates Oklander, L.I. Steinberg, E.R. Mudry, M.D. Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates. Fil:Oklander, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Steinberg, E.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates
spellingShingle	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates Oklander, L.I. Steinberg, E.R. Mudry, M.D. A new world monkey microsatellite (AP74) higly conserved in primates
topic_facet	Genetic variability Genomic conservation Microsatellite markers Non-invasive sampling PCR amplification Primates Alouatta Alouatta caraya Ateles belzebuth Ateles belzebuth chamek Ateles sp. Haplorhini Homo sapiens Lemur Lemur catta Lemuridae Pan troglodytes Papio Papio sp. Platyrrhini Primates
description	Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.
format	JOUR
author	Oklander, L.I. Steinberg, E.R. Mudry, M.D.
author_facet	Oklander, L.I. Steinberg, E.R. Mudry, M.D.
author_sort	Oklander, L.I.
title	A new world monkey microsatellite (AP74) higly conserved in primates
title_short	A new world monkey microsatellite (AP74) higly conserved in primates
title_full	A new world monkey microsatellite (AP74) higly conserved in primates
title_fullStr	A new world monkey microsatellite (AP74) higly conserved in primates
title_full_unstemmed	A new world monkey microsatellite (AP74) higly conserved in primates
title_sort	new world monkey microsatellite (ap74) higly conserved in primates
url	http://hdl.handle.net/20.500.12110/paper_0120548X_v17_n1_p93_Oklander
work_keys_str_mv	AT oklanderli anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT steinberger anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT mudrymd anewworldmonkeymicrosatelliteap74higlyconservedinprimates AT oklanderli newworldmonkeymicrosatelliteap74higlyconservedinprimates AT steinberger newworldmonkeymicrosatelliteap74higlyconservedinprimates AT mudrymd newworldmonkeymicrosatelliteap74higlyconservedinprimates
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A new world monkey microsatellite (AP74) higly conserved in primates

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