Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact...
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todo:paper_00992240_v81_n3_p1013_Russo2023-10-03T14:57:12Z Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum Russo, D.M. Abdian, P.L. Posadas, D.M. Williams, A. Vozza, N. Giordano, W. Kannenberg, E. Allan Downie, J. Zorreguietaa, A. Adhesion Cell adhesion Cell membranes Chains Compaction Adhesive properties Cell aggregates Cell-cell interaction Cell-to-cell interactions Exopolysaccharides Lipopolysaccharides Rhizobium leguminosarum Three-dimensional structure Biofilms antigen biofilm colony structure germ cell mutation polysaccharide rhizobacterium survivorship Bacteria (microorganisms) Rhizobium leguminosarum lipopolysaccharide O antigen biofilm DNA sequence genetics growth, development and aging metabolism microbiology molecular genetics physiology plant root Rhizobium leguminosarum Biofilms Lipopolysaccharides Molecular Sequence Data O Antigens Plant Roots Rhizobium leguminosarum Sequence Analysis, DNA The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces. © 2015, American Society for Microbiology. Fil:Russo, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abdian, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vozza, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v81_n3_p1013_Russo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adhesion Cell adhesion Cell membranes Chains Compaction Adhesive properties Cell aggregates Cell-cell interaction Cell-to-cell interactions Exopolysaccharides Lipopolysaccharides Rhizobium leguminosarum Three-dimensional structure Biofilms antigen biofilm colony structure germ cell mutation polysaccharide rhizobacterium survivorship Bacteria (microorganisms) Rhizobium leguminosarum lipopolysaccharide O antigen biofilm DNA sequence genetics growth, development and aging metabolism microbiology molecular genetics physiology plant root Rhizobium leguminosarum Biofilms Lipopolysaccharides Molecular Sequence Data O Antigens Plant Roots Rhizobium leguminosarum Sequence Analysis, DNA |
| spellingShingle |
Adhesion Cell adhesion Cell membranes Chains Compaction Adhesive properties Cell aggregates Cell-cell interaction Cell-to-cell interactions Exopolysaccharides Lipopolysaccharides Rhizobium leguminosarum Three-dimensional structure Biofilms antigen biofilm colony structure germ cell mutation polysaccharide rhizobacterium survivorship Bacteria (microorganisms) Rhizobium leguminosarum lipopolysaccharide O antigen biofilm DNA sequence genetics growth, development and aging metabolism microbiology molecular genetics physiology plant root Rhizobium leguminosarum Biofilms Lipopolysaccharides Molecular Sequence Data O Antigens Plant Roots Rhizobium leguminosarum Sequence Analysis, DNA Russo, D.M. Abdian, P.L. Posadas, D.M. Williams, A. Vozza, N. Giordano, W. Kannenberg, E. Allan Downie, J. Zorreguietaa, A. Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| topic_facet |
Adhesion Cell adhesion Cell membranes Chains Compaction Adhesive properties Cell aggregates Cell-cell interaction Cell-to-cell interactions Exopolysaccharides Lipopolysaccharides Rhizobium leguminosarum Three-dimensional structure Biofilms antigen biofilm colony structure germ cell mutation polysaccharide rhizobacterium survivorship Bacteria (microorganisms) Rhizobium leguminosarum lipopolysaccharide O antigen biofilm DNA sequence genetics growth, development and aging metabolism microbiology molecular genetics physiology plant root Rhizobium leguminosarum Biofilms Lipopolysaccharides Molecular Sequence Data O Antigens Plant Roots Rhizobium leguminosarum Sequence Analysis, DNA |
| description |
The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces. © 2015, American Society for Microbiology. |
| format |
JOUR |
| author |
Russo, D.M. Abdian, P.L. Posadas, D.M. Williams, A. Vozza, N. Giordano, W. Kannenberg, E. Allan Downie, J. Zorreguietaa, A. |
| author_facet |
Russo, D.M. Abdian, P.L. Posadas, D.M. Williams, A. Vozza, N. Giordano, W. Kannenberg, E. Allan Downie, J. Zorreguietaa, A. |
| author_sort |
Russo, D.M. |
| title |
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| title_short |
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| title_full |
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| title_fullStr |
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| title_full_unstemmed |
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum |
| title_sort |
lipopolysaccharide o-chain core region required for cellular cohesion and compaction of in vitro and root biofilms developed by rhizobium leguminosarum |
| url |
http://hdl.handle.net/20.500.12110/paper_00992240_v81_n3_p1013_Russo |
| work_keys_str_mv |
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1807320186222542848 |