Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356

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We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence com...

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Autores principales:	Acosta, M.P., Palomino, M.M., Allievi, M.C., Rivas, C.S., Ruzal, S.M.
Formato:	JOUR
Materias:	Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme analysis nonhuman nucleotide sequence Western blotting zymography Bacillus subtilis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00992240_v74_n24_p7824_Acosta
record_format	dspace
spelling	todo:paper_00992240_v74_n24_p7824_Acosta2023-10-03T14:57:08Z Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 Acosta, M.P. Palomino, M.M. Allievi, M.C. Rivas, C.S. Ruzal, S.M. Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity. Copyright © 2008, American Society for Microbiology. All Rights Reserved. Fil:Acosta, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica
spellingShingle	Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica Acosta, M.P. Palomino, M.M. Allievi, M.C. Rivas, C.S. Ruzal, S.M. Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
topic_facet	Bacilli Bacteriology Salmonella Amino acid sequence C-terminal domains Cloning and expression Enzymatic activities Functional verification Hydrolase activities Lactobacillus acidophilus Salmonella enterica Hydrolases bacterial enzyme hydrolase peptidoglycan hydrolase unclassified drug amino acid bacterium bioassay enzyme activity functional role gene expression amino acid sequence article carboxy terminal sequence enzyme activity enzyme analysis Lactobacillus acidophilus nonhuman nucleotide sequence Salmonella enterica Western blotting zymography Bacillus subtilis Lactobacillus acidophilus Salmonella enterica
description	We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
format	JOUR
author	Acosta, M.P. Palomino, M.M. Allievi, M.C. Rivas, C.S. Ruzal, S.M.
author_facet	Acosta, M.P. Palomino, M.M. Allievi, M.C. Rivas, C.S. Ruzal, S.M.
author_sort	Acosta, M.P.
title	Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
title_short	Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
title_full	Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
title_fullStr	Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
title_full_unstemmed	Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
title_sort	murein hydrolase activity in the surface layer of lactobacillus acidophilus atcc 4356
url	http://hdl.handle.net/20.500.12110/paper_00992240_v74_n24_p7824_Acosta
work_keys_str_mv	AT acostamp mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT palominomm mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT allievimc mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT rivascs mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356 AT ruzalsm mureinhydrolaseactivityinthesurfacelayeroflactobacillusacidophilusatcc4356
_version_	1782027333505384448

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356

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