Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles pre...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00980331_v41_n4_p360_Brunetti |
| Aporte de: |
| id |
todo:paper_00980331_v41_n4_p360_Brunetti |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00980331_v41_n4_p360_Brunetti2023-10-03T14:56:55Z Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs Brunetti, A.E. Merib, J. Carasek, E. Caramão, E.B. Barbará, J. Zini, C.A. Faivovich, J. Amphibia Anura Chemical defense Glands Hylidae Skin Smell Solid phase micro extraction volatile organic compound analysis animal Anura chemistry female male mass fragmentography metabolism odor skin solid phase microextraction species difference Animals Anura Female Gas Chromatography-Mass Spectrometry Male Odors Skin Solid Phase Microextraction Species Specificity Volatile Organic Compounds A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents). © 2015, Springer Science+Business Media New York. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00980331_v41_n4_p360_Brunetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amphibia Anura Chemical defense Glands Hylidae Skin Smell Solid phase micro extraction volatile organic compound analysis animal Anura chemistry female male mass fragmentography metabolism odor skin solid phase microextraction species difference Animals Anura Female Gas Chromatography-Mass Spectrometry Male Odors Skin Solid Phase Microextraction Species Specificity Volatile Organic Compounds |
| spellingShingle |
Amphibia Anura Chemical defense Glands Hylidae Skin Smell Solid phase micro extraction volatile organic compound analysis animal Anura chemistry female male mass fragmentography metabolism odor skin solid phase microextraction species difference Animals Anura Female Gas Chromatography-Mass Spectrometry Male Odors Skin Solid Phase Microextraction Species Specificity Volatile Organic Compounds Brunetti, A.E. Merib, J. Carasek, E. Caramão, E.B. Barbará, J. Zini, C.A. Faivovich, J. Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| topic_facet |
Amphibia Anura Chemical defense Glands Hylidae Skin Smell Solid phase micro extraction volatile organic compound analysis animal Anura chemistry female male mass fragmentography metabolism odor skin solid phase microextraction species difference Animals Anura Female Gas Chromatography-Mass Spectrometry Male Odors Skin Solid Phase Microextraction Species Specificity Volatile Organic Compounds |
| description |
A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents). © 2015, Springer Science+Business Media New York. |
| format |
JOUR |
| author |
Brunetti, A.E. Merib, J. Carasek, E. Caramão, E.B. Barbará, J. Zini, C.A. Faivovich, J. |
| author_facet |
Brunetti, A.E. Merib, J. Carasek, E. Caramão, E.B. Barbará, J. Zini, C.A. Faivovich, J. |
| author_sort |
Brunetti, A.E. |
| title |
Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| title_short |
Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| title_full |
Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| title_fullStr |
Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| title_full_unstemmed |
Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs |
| title_sort |
frog volatile compounds: application of in vivo spme for the characterization of the odorous secretions from two species of hypsiboas treefrogs |
| url |
http://hdl.handle.net/20.500.12110/paper_00980331_v41_n4_p360_Brunetti |
| work_keys_str_mv |
AT brunettiae frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT meribj frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT caraseke frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT caramaoeb frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT barbaraj frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT zinica frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs AT faivovichj frogvolatilecompoundsapplicationofinvivospmeforthecharacterizationoftheodoroussecretionsfromtwospeciesofhypsiboastreefrogs |
| _version_ |
1807321281561886720 |