Chemical constituents and biological activity of euglena gracilis extracts
Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements, suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extr...
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todo:paper_00974694_v7_n3_p209_Tolivia2023-10-03T14:56:48Z Chemical constituents and biological activity of euglena gracilis extracts Tolivia, A. Conforti, V. Córdoba, O. Flores, L. Antioxidant activity Euglena gracilis Growth inhibition activity Phytochemical screening Polyphenols 1,1 diphenyl 2 picrylhydrazyl algal extract alpha cryptoxanthin antheraxantin anthraquinone derivative beta carotene beta cryptoxanthin carbohydrate derivative cardenolide derivative carotenoid chlorophyll a chlorophyll b corticosteroid derivative cryptoxanthin diadinoxanthin Euglena gracilis extract flavonoid flavonol derivative fucoxantol lipid microxanthin neoxanthin pigment polyphenol derivative quercetin tannin derivative triterpene derivative unclassified drug unindexed drug violaxanthin xanthophyll antineoplastic activity antioxidant activity article biological activity bleaching chemical composition chlorophyll content chromatography drug isolation drug screening drug synthesis Euglena gracilis growth curve growth inhibition in vitro study nonhuman photosynthesis phytochemistry priority journal wild type Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements, suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH• and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products. © 2013, JPR Solutions. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00974694_v7_n3_p209_Tolivia |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant activity Euglena gracilis Growth inhibition activity Phytochemical screening Polyphenols 1,1 diphenyl 2 picrylhydrazyl algal extract alpha cryptoxanthin antheraxantin anthraquinone derivative beta carotene beta cryptoxanthin carbohydrate derivative cardenolide derivative carotenoid chlorophyll a chlorophyll b corticosteroid derivative cryptoxanthin diadinoxanthin Euglena gracilis extract flavonoid flavonol derivative fucoxantol lipid microxanthin neoxanthin pigment polyphenol derivative quercetin tannin derivative triterpene derivative unclassified drug unindexed drug violaxanthin xanthophyll antineoplastic activity antioxidant activity article biological activity bleaching chemical composition chlorophyll content chromatography drug isolation drug screening drug synthesis Euglena gracilis growth curve growth inhibition in vitro study nonhuman photosynthesis phytochemistry priority journal wild type |
| spellingShingle |
Antioxidant activity Euglena gracilis Growth inhibition activity Phytochemical screening Polyphenols 1,1 diphenyl 2 picrylhydrazyl algal extract alpha cryptoxanthin antheraxantin anthraquinone derivative beta carotene beta cryptoxanthin carbohydrate derivative cardenolide derivative carotenoid chlorophyll a chlorophyll b corticosteroid derivative cryptoxanthin diadinoxanthin Euglena gracilis extract flavonoid flavonol derivative fucoxantol lipid microxanthin neoxanthin pigment polyphenol derivative quercetin tannin derivative triterpene derivative unclassified drug unindexed drug violaxanthin xanthophyll antineoplastic activity antioxidant activity article biological activity bleaching chemical composition chlorophyll content chromatography drug isolation drug screening drug synthesis Euglena gracilis growth curve growth inhibition in vitro study nonhuman photosynthesis phytochemistry priority journal wild type Tolivia, A. Conforti, V. Córdoba, O. Flores, L. Chemical constituents and biological activity of euglena gracilis extracts |
| topic_facet |
Antioxidant activity Euglena gracilis Growth inhibition activity Phytochemical screening Polyphenols 1,1 diphenyl 2 picrylhydrazyl algal extract alpha cryptoxanthin antheraxantin anthraquinone derivative beta carotene beta cryptoxanthin carbohydrate derivative cardenolide derivative carotenoid chlorophyll a chlorophyll b corticosteroid derivative cryptoxanthin diadinoxanthin Euglena gracilis extract flavonoid flavonol derivative fucoxantol lipid microxanthin neoxanthin pigment polyphenol derivative quercetin tannin derivative triterpene derivative unclassified drug unindexed drug violaxanthin xanthophyll antineoplastic activity antioxidant activity article biological activity bleaching chemical composition chlorophyll content chromatography drug isolation drug screening drug synthesis Euglena gracilis growth curve growth inhibition in vitro study nonhuman photosynthesis phytochemistry priority journal wild type |
| description |
Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements, suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH• and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products. © 2013, JPR Solutions. |
| format |
JOUR |
| author |
Tolivia, A. Conforti, V. Córdoba, O. Flores, L. |
| author_facet |
Tolivia, A. Conforti, V. Córdoba, O. Flores, L. |
| author_sort |
Tolivia, A. |
| title |
Chemical constituents and biological activity of euglena gracilis extracts |
| title_short |
Chemical constituents and biological activity of euglena gracilis extracts |
| title_full |
Chemical constituents and biological activity of euglena gracilis extracts |
| title_fullStr |
Chemical constituents and biological activity of euglena gracilis extracts |
| title_full_unstemmed |
Chemical constituents and biological activity of euglena gracilis extracts |
| title_sort |
chemical constituents and biological activity of euglena gracilis extracts |
| url |
http://hdl.handle.net/20.500.12110/paper_00974694_v7_n3_p209_Tolivia |
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1807315069662396416 |