AFLP characterization in pathogenic and coprophilous fungi

The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four Msel +1 primers were assayed in combination with...

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Autores principales: Cinto, I.E., Gottlieb, A.M., Gally, M., Ranalli, M.E., Ramos, A.M.
Formato: JOUR
Materias:
Ascomycetes
Genetic characterization
Molecular markers
Taxonomy
Ascobolaceae
Ascomycota
Colletotrichum
Coprophilous
Fungi
Iodophanus
Pezizaceae
Saccobolus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00934666_v110_n_p81_Cinto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00934666_v110_n_p81_Cinto
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spelling todo:paper_00934666_v110_n_p81_Cinto2023-10-03T14:55:37Z AFLP characterization in pathogenic and coprophilous fungi Cinto, I.E. Gottlieb, A.M. Gally, M. Ranalli, M.E. Ramos, A.M. Ascomycetes Genetic characterization Molecular markers Taxonomy Ascobolaceae Ascomycota Colletotrichum Coprophilous Fungi Iodophanus Pezizaceae Saccobolus The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four Msel +1 primers were assayed in combination with two EcoRI +2 and four EcoRI +3 primers. In the present study both +2 and +3 EcoRI primers were informative, but EcoRI +2 produced profiles with high complexity. The addition of the extra selective nucleotide reduced the complexity of the banding patterns generating easily readable patterns to evaluate genetic diversity within and among species. Of the three ascomycetous genera assessed in this study, Colletotrichum (Glomerellaceae) presented the highest proportion of polymorphic AFLP loci, followed in order by Iodophanus (Pezizaceae) and Saccobolus (Ascobolaceae). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00934666_v110_n_p81_Cinto
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascomycetes
Genetic characterization
Molecular markers
Taxonomy
Ascobolaceae
Ascomycota
Colletotrichum
Coprophilous
Fungi
Iodophanus
Pezizaceae
Saccobolus
spellingShingle Ascomycetes
Genetic characterization
Molecular markers
Taxonomy
Ascobolaceae
Ascomycota
Colletotrichum
Coprophilous
Fungi
Iodophanus
Pezizaceae
Saccobolus
Cinto, I.E.
Gottlieb, A.M.
Gally, M.
Ranalli, M.E.
Ramos, A.M.
AFLP characterization in pathogenic and coprophilous fungi
topic_facet Ascomycetes
Genetic characterization
Molecular markers
Taxonomy
Ascobolaceae
Ascomycota
Colletotrichum
Coprophilous
Fungi
Iodophanus
Pezizaceae
Saccobolus
description The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four Msel +1 primers were assayed in combination with two EcoRI +2 and four EcoRI +3 primers. In the present study both +2 and +3 EcoRI primers were informative, but EcoRI +2 produced profiles with high complexity. The addition of the extra selective nucleotide reduced the complexity of the banding patterns generating easily readable patterns to evaluate genetic diversity within and among species. Of the three ascomycetous genera assessed in this study, Colletotrichum (Glomerellaceae) presented the highest proportion of polymorphic AFLP loci, followed in order by Iodophanus (Pezizaceae) and Saccobolus (Ascobolaceae).
format JOUR
author Cinto, I.E.
Gottlieb, A.M.
Gally, M.
Ranalli, M.E.
Ramos, A.M.
author_facet Cinto, I.E.
Gottlieb, A.M.
Gally, M.
Ranalli, M.E.
Ramos, A.M.
author_sort Cinto, I.E.
title AFLP characterization in pathogenic and coprophilous fungi
title_short AFLP characterization in pathogenic and coprophilous fungi
title_full AFLP characterization in pathogenic and coprophilous fungi
title_fullStr AFLP characterization in pathogenic and coprophilous fungi
title_full_unstemmed AFLP characterization in pathogenic and coprophilous fungi
title_sort aflp characterization in pathogenic and coprophilous fungi
url http://hdl.handle.net/20.500.12110/paper_00934666_v110_n_p81_Cinto
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