Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus

A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pedreira, M.E., Dimant, B., Tomsic, D., Quesada-Allue, L.A., Maldonado, H.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira
Aporte de:
id todo:paper_00913057_v52_n2_p385_Pedreira
record_format dspace
spelling todo:paper_00913057_v52_n2_p385_Pedreira2023-10-03T14:54:56Z Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus Pedreira, M.E. Dimant, B. Tomsic, D. Quesada-Allue, L.A. Maldonado, H. Arthropoda Crab Cycloheximide Habituation Memory amino acid mixture cycloheximide protein synthesis inhibitor radioisotope amnesia animal experiment animal tissue article brain controlled study crab escape behavior ganglion habituation long term memory male nonhuman priority journal protein synthesis short term memory training Amino Acids Amnesia Animal Brachyura Conditioning, Operant Cycloheximide Escape Reaction Ganglia, Invertebrate Habituation (Psychophysiology) Male Memory Protein Synthesis Inhibitors Support, Non-U.S. Gov't Animalia Arthropoda Chasmagnathus Decapoda (Crustacea) A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorporation into cerebral plus thoracic ganglia by 88% and 20 μg by 92%, but no inhibition was found at 24 h. A single injection of 10-20 μg, CY given 30 min before training, failed to affect the short-term habituation. Similar doses impaired both context memory (CM) and long-term habituation (LTH) when tested at 72 and 120 h but only CM at 24 h. Such a disparity was explained by an unspecific depressing effect upon the response, attributed to an interaction between CY and training. The hypothesis was confirmed, because CY injected immediately after training disclosed amnestic effect at 24 h on both CM and LTH. A similar effect was proven when animals were injected at 2 h but not at 6 h after training. Results from experiments with pretraining and pretesting injections put aside a state-dependence or retrieval deficit effects of the drug. Taken together, findings of this article argue strongly for de novo protein synthesis as a mechanism of LTH and for the close relation between CM and LTH. © 1995. Fil:Pedreira, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dimant, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tomsic, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allue, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Arthropoda
Crab
Cycloheximide
Habituation
Memory
amino acid mixture
cycloheximide
protein synthesis inhibitor
radioisotope
amnesia
animal experiment
animal tissue
article
brain
controlled study
crab
escape behavior
ganglion
habituation
long term memory
male
nonhuman
priority journal
protein synthesis
short term memory
training
Amino Acids
Amnesia
Animal
Brachyura
Conditioning, Operant
Cycloheximide
Escape Reaction
Ganglia, Invertebrate
Habituation (Psychophysiology)
Male
Memory
Protein Synthesis Inhibitors
Support, Non-U.S. Gov't
Animalia
Arthropoda
Chasmagnathus
Decapoda (Crustacea)
spellingShingle Arthropoda
Crab
Cycloheximide
Habituation
Memory
amino acid mixture
cycloheximide
protein synthesis inhibitor
radioisotope
amnesia
animal experiment
animal tissue
article
brain
controlled study
crab
escape behavior
ganglion
habituation
long term memory
male
nonhuman
priority journal
protein synthesis
short term memory
training
Amino Acids
Amnesia
Animal
Brachyura
Conditioning, Operant
Cycloheximide
Escape Reaction
Ganglia, Invertebrate
Habituation (Psychophysiology)
Male
Memory
Protein Synthesis Inhibitors
Support, Non-U.S. Gov't
Animalia
Arthropoda
Chasmagnathus
Decapoda (Crustacea)
Pedreira, M.E.
Dimant, B.
Tomsic, D.
Quesada-Allue, L.A.
Maldonado, H.
Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
topic_facet Arthropoda
Crab
Cycloheximide
Habituation
Memory
amino acid mixture
cycloheximide
protein synthesis inhibitor
radioisotope
amnesia
animal experiment
animal tissue
article
brain
controlled study
crab
escape behavior
ganglion
habituation
long term memory
male
nonhuman
priority journal
protein synthesis
short term memory
training
Amino Acids
Amnesia
Animal
Brachyura
Conditioning, Operant
Cycloheximide
Escape Reaction
Ganglia, Invertebrate
Habituation (Psychophysiology)
Male
Memory
Protein Synthesis Inhibitors
Support, Non-U.S. Gov't
Animalia
Arthropoda
Chasmagnathus
Decapoda (Crustacea)
description A shadow moving over head elicits an escape response in the crab Chasmagnathus that habituates promptly and for a long period. The effect of the protein synthesis inhibitor Cycloheximide (CY) on this long-term memory was analyzed. Two hours after injection, 10 μg CY inhibited [14C]-amino acid incorporation into cerebral plus thoracic ganglia by 88% and 20 μg by 92%, but no inhibition was found at 24 h. A single injection of 10-20 μg, CY given 30 min before training, failed to affect the short-term habituation. Similar doses impaired both context memory (CM) and long-term habituation (LTH) when tested at 72 and 120 h but only CM at 24 h. Such a disparity was explained by an unspecific depressing effect upon the response, attributed to an interaction between CY and training. The hypothesis was confirmed, because CY injected immediately after training disclosed amnestic effect at 24 h on both CM and LTH. A similar effect was proven when animals were injected at 2 h but not at 6 h after training. Results from experiments with pretraining and pretesting injections put aside a state-dependence or retrieval deficit effects of the drug. Taken together, findings of this article argue strongly for de novo protein synthesis as a mechanism of LTH and for the close relation between CM and LTH. © 1995.
format JOUR
author Pedreira, M.E.
Dimant, B.
Tomsic, D.
Quesada-Allue, L.A.
Maldonado, H.
author_facet Pedreira, M.E.
Dimant, B.
Tomsic, D.
Quesada-Allue, L.A.
Maldonado, H.
author_sort Pedreira, M.E.
title Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
title_short Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
title_full Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
title_fullStr Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
title_full_unstemmed Cycloheximide inhibits context memory and long-term habituation in the crab Chasmagnathus
title_sort cycloheximide inhibits context memory and long-term habituation in the crab chasmagnathus
url http://hdl.handle.net/20.500.12110/paper_00913057_v52_n2_p385_Pedreira
work_keys_str_mv AT pedreirame cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus
AT dimantb cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus
AT tomsicd cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus
AT quesadaalluela cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus
AT maldonadoh cycloheximideinhibitscontextmemoryandlongtermhabituationinthecrabchasmagnathus
_version_ 1807320584424521728