Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone
The accumulation and output of 3H -prostaglandins (PGs), E2 and F2α, into and from uterine strips isolated from ovariectomized rats, either in presence or in absence of exogenous progesterone, were explored. Tissue-to-medium ratio of 3H - counts (T/M-ratio), was determined. The same was done in solu...
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todo:paper_00906980_v33_n1_p51_Gimeno2023-10-03T14:54:50Z Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone Gimeno, M.F. Franchi, A.M. González, E.T. Gimeno, A.L. progesterone prostaglandin E prostaglandin E2 prostaglandin F prostaglandin F2 alpha progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope sucrose c 14 unclassified drug animal article female in vitro study metabolism ovariectomy rat uterus animal cell drug administration drug efficacy drug mechanism drug receptor binding etiology female genital system muscle nonhuman priority journal prostaglandin e2 h 3 prostaglandin f2 alpha h 3 sex difference sexual cycle uterus Animal Dinoprost Dinoprostone Female In Vitro Ovariectomy Progesterone Prostaglandins E Prostaglandins F Rats Support, Non-U.S. Gov't Uterus The accumulation and output of 3H -prostaglandins (PGs), E2 and F2α, into and from uterine strips isolated from ovariectomized rats, either in presence or in absence of exogenous progesterone, were explored. Tissue-to-medium ratio of 3H - counts (T/M-ratio), was determined. The same was done in solutions containing 14C-sucrose. During a 60 min incubation period in a solution containing 3H -PGF2α, a net accumulation of radioactivity was evident in control (no progesterone) uterine slices. The T/M-ratio for 3H-PGF2α, increased with time, reaching maximal values at 45 min. Progesterone (100 ug.ml-1) attenuated the uptake process, as evidenced by stable values of T/M-ratio, as time progressed. On the other hand, control T/M-ratio for fluenced by the presence of exogenous progesterone. Regarding labelled PG release from the tissue, it was observed that, during an experimental period of 60 min, most tritium from control slices was released within the first 30 min after incubation with 3H -PGF2α, whereas, following the presence and subsequent removal of exogenous progesterone, the bulk of 3H -released took place at 6-70 min. On the other hand, the release of 3H after an incubation with 3H -PGE2, was also maximal as that for 3H -PGF2, α within the first 30 min and resulted not altered after a period of exposure and removal of progesterone. The foregoing results suggest an specific pharmacological effect of progesterone, attenuating the uptake and retarding the outflow of PGF2α, but not that of PGE2, into and from uterine slices of ovariectomized rats. Findings reported herein are discussed in terms of progesterone priming and withdrawal, in relation to PGF2α fluxes in the rat uterus during the sex cycle, as well as in relation to PG binding to tissue receptors. © 1987. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00906980_v33_n1_p51_Gimeno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
progesterone prostaglandin E prostaglandin E2 prostaglandin F prostaglandin F2 alpha progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope sucrose c 14 unclassified drug animal article female in vitro study metabolism ovariectomy rat uterus animal cell drug administration drug efficacy drug mechanism drug receptor binding etiology female genital system muscle nonhuman priority journal prostaglandin e2 h 3 prostaglandin f2 alpha h 3 sex difference sexual cycle uterus Animal Dinoprost Dinoprostone Female In Vitro Ovariectomy Progesterone Prostaglandins E Prostaglandins F Rats Support, Non-U.S. Gov't Uterus |
| spellingShingle |
progesterone prostaglandin E prostaglandin E2 prostaglandin F prostaglandin F2 alpha progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope sucrose c 14 unclassified drug animal article female in vitro study metabolism ovariectomy rat uterus animal cell drug administration drug efficacy drug mechanism drug receptor binding etiology female genital system muscle nonhuman priority journal prostaglandin e2 h 3 prostaglandin f2 alpha h 3 sex difference sexual cycle uterus Animal Dinoprost Dinoprostone Female In Vitro Ovariectomy Progesterone Prostaglandins E Prostaglandins F Rats Support, Non-U.S. Gov't Uterus Gimeno, M.F. Franchi, A.M. González, E.T. Gimeno, A.L. Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| topic_facet |
progesterone prostaglandin E prostaglandin E2 prostaglandin F prostaglandin F2 alpha progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope sucrose c 14 unclassified drug animal article female in vitro study metabolism ovariectomy rat uterus animal cell drug administration drug efficacy drug mechanism drug receptor binding etiology female genital system muscle nonhuman priority journal prostaglandin e2 h 3 prostaglandin f2 alpha h 3 sex difference sexual cycle uterus Animal Dinoprost Dinoprostone Female In Vitro Ovariectomy Progesterone Prostaglandins E Prostaglandins F Rats Support, Non-U.S. Gov't Uterus |
| description |
The accumulation and output of 3H -prostaglandins (PGs), E2 and F2α, into and from uterine strips isolated from ovariectomized rats, either in presence or in absence of exogenous progesterone, were explored. Tissue-to-medium ratio of 3H - counts (T/M-ratio), was determined. The same was done in solutions containing 14C-sucrose. During a 60 min incubation period in a solution containing 3H -PGF2α, a net accumulation of radioactivity was evident in control (no progesterone) uterine slices. The T/M-ratio for 3H-PGF2α, increased with time, reaching maximal values at 45 min. Progesterone (100 ug.ml-1) attenuated the uptake process, as evidenced by stable values of T/M-ratio, as time progressed. On the other hand, control T/M-ratio for fluenced by the presence of exogenous progesterone. Regarding labelled PG release from the tissue, it was observed that, during an experimental period of 60 min, most tritium from control slices was released within the first 30 min after incubation with 3H -PGF2α, whereas, following the presence and subsequent removal of exogenous progesterone, the bulk of 3H -released took place at 6-70 min. On the other hand, the release of 3H after an incubation with 3H -PGE2, was also maximal as that for 3H -PGF2, α within the first 30 min and resulted not altered after a period of exposure and removal of progesterone. The foregoing results suggest an specific pharmacological effect of progesterone, attenuating the uptake and retarding the outflow of PGF2α, but not that of PGE2, into and from uterine slices of ovariectomized rats. Findings reported herein are discussed in terms of progesterone priming and withdrawal, in relation to PGF2α fluxes in the rat uterus during the sex cycle, as well as in relation to PG binding to tissue receptors. © 1987. |
| format |
JOUR |
| author |
Gimeno, M.F. Franchi, A.M. González, E.T. Gimeno, A.L. |
| author_facet |
Gimeno, M.F. Franchi, A.M. González, E.T. Gimeno, A.L. |
| author_sort |
Gimeno, M.F. |
| title |
Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| title_short |
Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| title_full |
Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| title_fullStr |
Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| title_full_unstemmed |
Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| title_sort |
uptake and release of 3h prostaglandins e2 and f2α in uterin strips isolated from ovariectomized rats. influence of in vitro progesterone |
| url |
http://hdl.handle.net/20.500.12110/paper_00906980_v33_n1_p51_Gimeno |
| work_keys_str_mv |
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