Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats

Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spaye...

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Autores principales: Franchi, A.M., Gimeno, M.F., Gimeno, A.L.
Formato: JOUR
Materias:
estradiol
progesterone
prostaglandin e2
prostaglandin f2 alpha
radioisotope
animal cell
drug efficacy
endocrine system
female genital system
myometrium
nonhuman
ovariectomy
priority journal
prostaglandin e2 h 3
rat
Animal
Castration
Dinoprost
Dinoprostone
Estradiol
Female
NADP
Progesterone
Prostaglandins E
Prostaglandins F
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uterus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00906980_v29_n5_p773_Franchi2023-10-03T14:54:49Z Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats Franchi, A.M. Gimeno, M.F. Gimeno, A.L. estradiol progesterone prostaglandin e2 prostaglandin f2 alpha radioisotope animal cell drug efficacy endocrine system female genital system myometrium nonhuman ovariectomy priority journal prostaglandin e2 h 3 rat Animal Castration Dinoprost Dinoprostone Estradiol Female NADP Progesterone Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uterus Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic estradiol
progesterone
prostaglandin e2
prostaglandin f2 alpha
radioisotope
animal cell
drug efficacy
endocrine system
female genital system
myometrium
nonhuman
ovariectomy
priority journal
prostaglandin e2 h 3
rat
Animal
Castration
Dinoprost
Dinoprostone
Estradiol
Female
NADP
Progesterone
Prostaglandins E
Prostaglandins F
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uterus
spellingShingle estradiol
progesterone
prostaglandin e2
prostaglandin f2 alpha
radioisotope
animal cell
drug efficacy
endocrine system
female genital system
myometrium
nonhuman
ovariectomy
priority journal
prostaglandin e2 h 3
rat
Animal
Castration
Dinoprost
Dinoprostone
Estradiol
Female
NADP
Progesterone
Prostaglandins E
Prostaglandins F
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uterus
Franchi, A.M.
Gimeno, M.F.
Gimeno, A.L.
Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
topic_facet estradiol
progesterone
prostaglandin e2
prostaglandin f2 alpha
radioisotope
animal cell
drug efficacy
endocrine system
female genital system
myometrium
nonhuman
ovariectomy
priority journal
prostaglandin e2 h 3
rat
Animal
Castration
Dinoprost
Dinoprostone
Estradiol
Female
NADP
Progesterone
Prostaglandins E
Prostaglandins F
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uterus
description Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985.
format JOUR
author Franchi, A.M.
Gimeno, M.F.
Gimeno, A.L.
author_facet Franchi, A.M.
Gimeno, M.F.
Gimeno, A.L.
author_sort Franchi, A.M.
title Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
title_short Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
title_full Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
title_fullStr Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
title_full_unstemmed Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
title_sort estradiol -17-β enhances the formation of {3h}-pgf2α from {3h}-pge2 in the uterus isolated from ovariectomized rats
url http://hdl.handle.net/20.500.12110/paper_00906980_v29_n5_p773_Franchi
work_keys_str_mv AT franchiam estradiol17benhancestheformationof3hpgf2afrom3hpge2intheuterusisolatedfromovariectomizedrats
AT gimenomf estradiol17benhancestheformationof3hpgf2afrom3hpge2intheuterusisolatedfromovariectomizedrats
AT gimenoal estradiol17benhancestheformationof3hpgf2afrom3hpge2intheuterusisolatedfromovariectomizedrats
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