Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats

Dose-response curves for several prostaglandins (PGI2; PGD2; PGF2 and PGE2); BaCl2 or prostaglandin metabolites (15-keto-PGF2α; 13, 14-diOH-15-keto-PGF2α; 6-keto-PGF1α and 6-keto-PGE1 in quiescent (indomethacin-treated) uterine strips from ovariectomized rats, were constructed. All PGs tested as wel...

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Autores principales:	Gimeno, A.L., Chaud, M., Bonacossa, A., Franchi, A.M., Gimeno, M.F.
Formato:	JOUR
Materias:	barium chloride indometacin prostacyclin prostaglandin d2 prostaglandin e2 prostaglandin f2 alpha animal cell biological model dose response dose time effect relation drug administration drug comparison drug efficacy drug response female genital system methodology myometrium nonhuman rat uterus contractility Animals Barium Barium Compounds Castration Chlorides Dinoprost Dinoprostone Dose-Response Relationship, Drug Epoprostenol Female Indomethacin Prostaglandin D2 Prostaglandins D Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00906980_v26_n4_p663_Gimeno
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00906980_v26_n4_p663_Gimeno2023-10-03T14:54:48Z Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats Gimeno, A.L. Chaud, M. Bonacossa, A. Franchi, A.M. Gimeno, M.F. barium chloride indometacin prostacyclin prostaglandin d2 prostaglandin e2 prostaglandin f2 alpha animal cell biological model dose response dose time effect relation drug administration drug comparison drug efficacy drug response female genital system methodology myometrium nonhuman rat uterus contractility Animals Barium Barium Compounds Castration Chlorides Dinoprost Dinoprostone Dose-Response Relationship, Drug Epoprostenol Female Indomethacin Prostaglandin D2 Prostaglandins D Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Animalia Dose-response curves for several prostaglandins (PGI2; PGD2; PGF2 and PGE2); BaCl2 or prostaglandin metabolites (15-keto-PGF2α; 13, 14-diOH-15-keto-PGF2α; 6-keto-PGF1α and 6-keto-PGE1 in quiescent (indomethacin-treated) uterine strips from ovariectomized rats, were constructed. All PGs tested as well as BaCl2, triggered at different concentrations, evident phasic contractions. Within the range of concentrations tested the portion of the curves for the metabolites of PGF2α was shifted to the right of that for PGF2α itself; the curve for 6-keto-PGF1α was displaced to the right of the curve for PGI2 and that for 6-keto-PGE1 to the left. It was also demonstrated that the uterine motility elicited by 10-5 M PGF2α and its metabolites was long lasting (more than 3 hours) and so it was the activity evoked by PGI2; 6-keto-PGF1α and BaCl2, but not the contractions following 6-keto-PGE1, which disappeared much earlier. The contractile tension after PGF2α; 15-keto-PGF2α; 13, 14-diOH-15-keto-PGF2α and PGI2, increased as time progressed whilst that evoked by 6-keto-PGF2α or BaCl2 fluctuated during the same period around more constant levels. The surprising sustained and gradually increasing contractile activity after a single dose of an unstable prostaglandin such as PGI2, on the isolated rat uterus rendered quiescent by indomethacin, is discussed in terms of an effect associated to its transformation into more stable metabolites (6-keto-PGF1α, or another not tested) or as a consequence of a factor which might protects prostacyclin from inactivation. © 1983. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00906980_v26_n4_p663_Gimeno
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	barium chloride indometacin prostacyclin prostaglandin d2 prostaglandin e2 prostaglandin f2 alpha animal cell biological model dose response dose time effect relation drug administration drug comparison drug efficacy drug response female genital system methodology myometrium nonhuman rat uterus contractility Animals Barium Barium Compounds Castration Chlorides Dinoprost Dinoprostone Dose-Response Relationship, Drug Epoprostenol Female Indomethacin Prostaglandin D2 Prostaglandins D Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Animalia
spellingShingle	barium chloride indometacin prostacyclin prostaglandin d2 prostaglandin e2 prostaglandin f2 alpha animal cell biological model dose response dose time effect relation drug administration drug comparison drug efficacy drug response female genital system methodology myometrium nonhuman rat uterus contractility Animals Barium Barium Compounds Castration Chlorides Dinoprost Dinoprostone Dose-Response Relationship, Drug Epoprostenol Female Indomethacin Prostaglandin D2 Prostaglandins D Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Animalia Gimeno, A.L. Chaud, M. Bonacossa, A. Franchi, A.M. Gimeno, M.F. Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
topic_facet	barium chloride indometacin prostacyclin prostaglandin d2 prostaglandin e2 prostaglandin f2 alpha animal cell biological model dose response dose time effect relation drug administration drug comparison drug efficacy drug response female genital system methodology myometrium nonhuman rat uterus contractility Animals Barium Barium Compounds Castration Chlorides Dinoprost Dinoprostone Dose-Response Relationship, Drug Epoprostenol Female Indomethacin Prostaglandin D2 Prostaglandins D Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Animalia
description	Dose-response curves for several prostaglandins (PGI2; PGD2; PGF2 and PGE2); BaCl2 or prostaglandin metabolites (15-keto-PGF2α; 13, 14-diOH-15-keto-PGF2α; 6-keto-PGF1α and 6-keto-PGE1 in quiescent (indomethacin-treated) uterine strips from ovariectomized rats, were constructed. All PGs tested as well as BaCl2, triggered at different concentrations, evident phasic contractions. Within the range of concentrations tested the portion of the curves for the metabolites of PGF2α was shifted to the right of that for PGF2α itself; the curve for 6-keto-PGF1α was displaced to the right of the curve for PGI2 and that for 6-keto-PGE1 to the left. It was also demonstrated that the uterine motility elicited by 10-5 M PGF2α and its metabolites was long lasting (more than 3 hours) and so it was the activity evoked by PGI2; 6-keto-PGF1α and BaCl2, but not the contractions following 6-keto-PGE1, which disappeared much earlier. The contractile tension after PGF2α; 15-keto-PGF2α; 13, 14-diOH-15-keto-PGF2α and PGI2, increased as time progressed whilst that evoked by 6-keto-PGF2α or BaCl2 fluctuated during the same period around more constant levels. The surprising sustained and gradually increasing contractile activity after a single dose of an unstable prostaglandin such as PGI2, on the isolated rat uterus rendered quiescent by indomethacin, is discussed in terms of an effect associated to its transformation into more stable metabolites (6-keto-PGF1α, or another not tested) or as a consequence of a factor which might protects prostacyclin from inactivation. © 1983.
format	JOUR
author	Gimeno, A.L. Chaud, M. Bonacossa, A. Franchi, A.M. Gimeno, M.F.
author_facet	Gimeno, A.L. Chaud, M. Bonacossa, A. Franchi, A.M. Gimeno, M.F.
author_sort	Gimeno, A.L.
title	Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
title_short	Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
title_full	Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
title_fullStr	Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
title_full_unstemmed	Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
title_sort	prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats
url	http://hdl.handle.net/20.500.12110/paper_00906980_v26_n4_p663_Gimeno
work_keys_str_mv	AT gimenoal prostacyclininducesasurprisinglonglastingmotilityinquiescentuterinestripsindomethacintreatedisolatedfromovariectomizedrats AT chaudm prostacyclininducesasurprisinglonglastingmotilityinquiescentuterinestripsindomethacintreatedisolatedfromovariectomizedrats AT bonacossaa prostacyclininducesasurprisinglonglastingmotilityinquiescentuterinestripsindomethacintreatedisolatedfromovariectomizedrats AT franchiam prostacyclininducesasurprisinglonglastingmotilityinquiescentuterinestripsindomethacintreatedisolatedfromovariectomizedrats AT gimenomf prostacyclininducesasurprisinglonglastingmotilityinquiescentuterinestripsindomethacintreatedisolatedfromovariectomizedrats
_version_	1782029196408651776

Prostacyclin induces a surprising long-lasting motility in quiescent uterine strips (indomethacin-treated) isolated from ovariectomized rats

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