Release and specific binding of prostaglandins in bovine pineal gland

Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affini...

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Autores principales: Cardinali, D.P., Ritta, M.N., Speziale, N.S., Gimeno, M.F.
Formato: JOUR
Materias:
Norepinephrine
Pineal Gland
Prostaglandin High Affinity Binding
Prostaglandin Receptors
Sympathetic Nervous System
indometacin
noradrenalin
prostaglandin
prostaglandin e1
prostaglandin e2
radioisotope
animal experiment
cattle
drug binding
drug release
endocrine system
in vitro study
pineal body
prostaglandin e2 h 3
prostaglandin f2 alpha h 3
radioimmunoassay
Animal
Cattle
Culture Media
Prostaglandins E
Prostaglandins F
Receptors, Prostaglandin
Secretory Rate
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00906980_v18_n4_p577_Cardinali
record_format dspace
spelling todo:paper_00906980_v18_n4_p577_Cardinali2023-10-03T14:54:45Z Release and specific binding of prostaglandins in bovine pineal gland Cardinali, D.P. Ritta, M.N. Speziale, N.S. Gimeno, M.F. Norepinephrine Pineal Gland Prostaglandin High Affinity Binding Prostaglandin Receptors Sympathetic Nervous System indometacin noradrenalin prostaglandin prostaglandin e1 prostaglandin e2 radioisotope animal experiment cattle drug binding drug release endocrine system in vitro study pineal body prostaglandin e2 h 3 prostaglandin f2 alpha h 3 radioimmunoassay Animal Cattle Culture Media Norepinephrine Pineal Gland Prostaglandins E Prostaglandins F Receptors, Prostaglandin Secretory Rate Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F2α (3H-PGF2α) in low speed supernatants of pineal homogenates. Binding was increased by increasing Ca++ concentration in medium up to 2 mM, was heat-labile and was depressed following incubation with trypsin. In subcellular fractionation studies maximal 3H-PG binding was found in the 27000 × g pellet. Scatchard analysis of 3H-PGE2 binding revealed the presence of a single population of binding sites with a Kd= 1.2 nM and a binding site concentration of 1-2 pmoles/g protein. A single population of binding sites for 3H-PGF2α was also detected with a Kd= 1.7 nM and a similar binding site concentration. Non-radioactive PGE1 and PGE2 were almost equally effective to compete for 3H-PGE2 binding sites (ED50= 5 and 2 nM, respectively). Unlabeled PGF2 was relatively ineffective to compete for 3H-PGE2 binding (ED50>1000 nM) but displaced effectively 3H-PGF2α binding (ED50= 1.2 nM). © 1979. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Norepinephrine
Pineal Gland
Prostaglandin High Affinity Binding
Prostaglandin Receptors
Sympathetic Nervous System
indometacin
noradrenalin
prostaglandin
prostaglandin e1
prostaglandin e2
radioisotope
animal experiment
cattle
drug binding
drug release
endocrine system
in vitro study
pineal body
prostaglandin e2 h 3
prostaglandin f2 alpha h 3
radioimmunoassay
Animal
Cattle
Culture Media
Norepinephrine
Pineal Gland
Prostaglandins E
Prostaglandins F
Receptors, Prostaglandin
Secretory Rate
spellingShingle Norepinephrine
Pineal Gland
Prostaglandin High Affinity Binding
Prostaglandin Receptors
Sympathetic Nervous System
indometacin
noradrenalin
prostaglandin
prostaglandin e1
prostaglandin e2
radioisotope
animal experiment
cattle
drug binding
drug release
endocrine system
in vitro study
pineal body
prostaglandin e2 h 3
prostaglandin f2 alpha h 3
radioimmunoassay
Animal
Cattle
Culture Media
Norepinephrine
Pineal Gland
Prostaglandins E
Prostaglandins F
Receptors, Prostaglandin
Secretory Rate
Cardinali, D.P.
Ritta, M.N.
Speziale, N.S.
Gimeno, M.F.
Release and specific binding of prostaglandins in bovine pineal gland
topic_facet Norepinephrine
Pineal Gland
Prostaglandin High Affinity Binding
Prostaglandin Receptors
Sympathetic Nervous System
indometacin
noradrenalin
prostaglandin
prostaglandin e1
prostaglandin e2
radioisotope
animal experiment
cattle
drug binding
drug release
endocrine system
in vitro study
pineal body
prostaglandin e2 h 3
prostaglandin f2 alpha h 3
radioimmunoassay
Animal
Cattle
Culture Media
Norepinephrine
Pineal Gland
Prostaglandins E
Prostaglandins F
Receptors, Prostaglandin
Secretory Rate
description Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F2α (3H-PGF2α) in low speed supernatants of pineal homogenates. Binding was increased by increasing Ca++ concentration in medium up to 2 mM, was heat-labile and was depressed following incubation with trypsin. In subcellular fractionation studies maximal 3H-PG binding was found in the 27000 × g pellet. Scatchard analysis of 3H-PGE2 binding revealed the presence of a single population of binding sites with a Kd= 1.2 nM and a binding site concentration of 1-2 pmoles/g protein. A single population of binding sites for 3H-PGF2α was also detected with a Kd= 1.7 nM and a similar binding site concentration. Non-radioactive PGE1 and PGE2 were almost equally effective to compete for 3H-PGE2 binding sites (ED50= 5 and 2 nM, respectively). Unlabeled PGF2 was relatively ineffective to compete for 3H-PGE2 binding (ED50>1000 nM) but displaced effectively 3H-PGF2α binding (ED50= 1.2 nM). © 1979.
format JOUR
author Cardinali, D.P.
Ritta, M.N.
Speziale, N.S.
Gimeno, M.F.
author_facet Cardinali, D.P.
Ritta, M.N.
Speziale, N.S.
Gimeno, M.F.
author_sort Cardinali, D.P.
title Release and specific binding of prostaglandins in bovine pineal gland
title_short Release and specific binding of prostaglandins in bovine pineal gland
title_full Release and specific binding of prostaglandins in bovine pineal gland
title_fullStr Release and specific binding of prostaglandins in bovine pineal gland
title_full_unstemmed Release and specific binding of prostaglandins in bovine pineal gland
title_sort release and specific binding of prostaglandins in bovine pineal gland
url http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali
work_keys_str_mv AT cardinalidp releaseandspecificbindingofprostaglandinsinbovinepinealgland
AT rittamn releaseandspecificbindingofprostaglandinsinbovinepinealgland
AT spezialens releaseandspecificbindingofprostaglandinsinbovinepinealgland
AT gimenomf releaseandspecificbindingofprostaglandinsinbovinepinealgland
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