Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) pe...
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todo:paper_00778923_v1040_n_p297_Denari2023-10-03T14:54:17Z Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes Denari, D. Ceballos, N.R. 11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences. Fil:Denari, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia |
spellingShingle |
11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia Denari, D. Ceballos, N.R. Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
topic_facet |
11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia |
description |
In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences. |
format |
SER |
author |
Denari, D. Ceballos, N.R. |
author_facet |
Denari, D. Ceballos, N.R. |
author_sort |
Denari, D. |
title |
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
title_short |
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
title_full |
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
title_fullStr |
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
title_full_unstemmed |
Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
title_sort |
seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes |
url |
http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari |
work_keys_str_mv |
AT denarid seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes AT ceballosnr seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes |
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1782029885198303232 |