Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes

In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) pe...

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Autores principales:	Denari, D., Ceballos, N.R.
Formato:	SER
Materias:	11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00778923_v1040_n_p297_Denari
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spelling	todo:paper_00778923_v1040_n_p297_Denari2023-10-03T14:54:17Z Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes Denari, D. Ceballos, N.R. 11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences. Fil:Denari, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia
spellingShingle	11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia Denari, D. Ceballos, N.R. Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
topic_facet	11β-hydroxysteroid dehydrogenase Corticosterone Testis Toad 11beta hydroxysteroid dehydrogenase corticosterone glucocorticoid glucose 6 phosphatase glycyrrhetinic acid nicotinamide adenine dinucleotide succinate dehydrogenase animal experiment animal tissue cell nucleus cellular distribution conference paper controlled study corticosterone blood level cytosol enzyme activity Leydig cell male Michaelis constant microsome mitochondrion nonhuman oxidation reproduction seasonal variation testis toad Anura Bufo arenarum Mammalia
description	In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences.
format	SER
author	Denari, D. Ceballos, N.R.
author_facet	Denari, D. Ceballos, N.R.
author_sort	Denari, D.
title	Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_short	Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_full	Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_fullStr	Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_full_unstemmed	Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
title_sort	seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
url	http://hdl.handle.net/20.500.12110/paper_00778923_v1040_n_p297_Denari
work_keys_str_mv	AT denarid seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes AT ceballosnr seasonalchangesintheactivityof11bhydroxysteroiddehydrogenaseintoadtestes
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Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes

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