Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?

Repair of damage and recovery of function are fundamental endeavors for recuperation of patients and experimental animals with spinal cord injury. Steroid hormones, such as progesterone (PROG), show regenerative and myelinating properties following injury of the peripheral and central nervous system...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	De Nicola, A.F., Labombarda, F., Gonzalez, S.L., Gonzalez Deniselle, M.C., Guennoun, R., Schumacher, M.
Formato:	SER
Materias:	Astrocytes Glial fibrillary acidic protein Myelin basic protein Neuroprotection NG2 cells Nitric oxide synthase Oligodendrocytes Progesterone Spinal cord injury glial fibrillary acidic protein myelin basic protein nitric oxide synthase progesterone progesterone receptor proteochondroitin sulfate reduced nicotinamide adenine dinucleotide phosphate dehydrogenase steroid animal cell animal experiment animal model animal tissue astrocyte cell activation cell count cell damage cell structure cell survival conference paper controlled study enzyme activation genomics glia cell hormone determination immunocytochemistry male motoneuron neuroprotection nonhuman oligodendroglia rat spinal cord function spinal cord injury spinal cord transsection Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00778923_v1007_n_p317_DeNicola
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00778923_v1007_n_p317_DeNicola
record_format	dspace
spelling	todo:paper_00778923_v1007_n_p317_DeNicola2023-10-03T14:54:17Z Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function? De Nicola, A.F. Labombarda, F. Gonzalez, S.L. Gonzalez Deniselle, M.C. Guennoun, R. Schumacher, M. Astrocytes Glial fibrillary acidic protein Myelin basic protein Neuroprotection NG2 cells Nitric oxide synthase Oligodendrocytes Progesterone Spinal cord injury glial fibrillary acidic protein myelin basic protein nitric oxide synthase progesterone progesterone receptor proteochondroitin sulfate reduced nicotinamide adenine dinucleotide phosphate dehydrogenase steroid animal cell animal experiment animal model animal tissue astrocyte cell activation cell count cell damage cell structure cell survival conference paper controlled study enzyme activation genomics glia cell hormone determination immunocytochemistry male motoneuron neuroprotection nonhuman oligodendroglia rat spinal cord function spinal cord injury spinal cord transsection Animalia Repair of damage and recovery of function are fundamental endeavors for recuperation of patients and experimental animals with spinal cord injury. Steroid hormones, such as progesterone (PROG), show regenerative and myelinating properties following injury of the peripheral and central nervous system. In this work, we studied PROG effects on glial cells of the normal and transected (TRX) spinal cord, to complement previous studies in motoneurons. Both neurons and glial cells expressed the classical PROG receptor (PR), suggesting that genomic mechanisms participated in PROG action. In TRX rats, PROG treatment stimulated the number of NADPH-diaphorase (nitric oxide synthase) active astrocytes, whereas the number of astrocytes expressing the glial fibrillary acidic protein (GFAP) was stimulated in control but not in TRX rats. PROG also stimulated the immunocytochemical staining for myelin-basic protein (MBP) and the number of oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2 in TRX rats. In terms of beneficial or detrimental consequences, these PROG effects may be supportive of neuronal recuperation, as shown for several neuronal functional parameters that were normalized by PROG treatment of spinal cord injured animals. Thus, PROG effects on glial cells go in parallel with morphological and biochemical evidence of survival of damaged motoneurons. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00778923_v1007_n_p317_DeNicola
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Astrocytes Glial fibrillary acidic protein Myelin basic protein Neuroprotection NG2 cells Nitric oxide synthase Oligodendrocytes Progesterone Spinal cord injury glial fibrillary acidic protein myelin basic protein nitric oxide synthase progesterone progesterone receptor proteochondroitin sulfate reduced nicotinamide adenine dinucleotide phosphate dehydrogenase steroid animal cell animal experiment animal model animal tissue astrocyte cell activation cell count cell damage cell structure cell survival conference paper controlled study enzyme activation genomics glia cell hormone determination immunocytochemistry male motoneuron neuroprotection nonhuman oligodendroglia rat spinal cord function spinal cord injury spinal cord transsection Animalia
spellingShingle	Astrocytes Glial fibrillary acidic protein Myelin basic protein Neuroprotection NG2 cells Nitric oxide synthase Oligodendrocytes Progesterone Spinal cord injury glial fibrillary acidic protein myelin basic protein nitric oxide synthase progesterone progesterone receptor proteochondroitin sulfate reduced nicotinamide adenine dinucleotide phosphate dehydrogenase steroid animal cell animal experiment animal model animal tissue astrocyte cell activation cell count cell damage cell structure cell survival conference paper controlled study enzyme activation genomics glia cell hormone determination immunocytochemistry male motoneuron neuroprotection nonhuman oligodendroglia rat spinal cord function spinal cord injury spinal cord transsection Animalia De Nicola, A.F. Labombarda, F. Gonzalez, S.L. Gonzalez Deniselle, M.C. Guennoun, R. Schumacher, M. Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
topic_facet	Astrocytes Glial fibrillary acidic protein Myelin basic protein Neuroprotection NG2 cells Nitric oxide synthase Oligodendrocytes Progesterone Spinal cord injury glial fibrillary acidic protein myelin basic protein nitric oxide synthase progesterone progesterone receptor proteochondroitin sulfate reduced nicotinamide adenine dinucleotide phosphate dehydrogenase steroid animal cell animal experiment animal model animal tissue astrocyte cell activation cell count cell damage cell structure cell survival conference paper controlled study enzyme activation genomics glia cell hormone determination immunocytochemistry male motoneuron neuroprotection nonhuman oligodendroglia rat spinal cord function spinal cord injury spinal cord transsection Animalia
description	Repair of damage and recovery of function are fundamental endeavors for recuperation of patients and experimental animals with spinal cord injury. Steroid hormones, such as progesterone (PROG), show regenerative and myelinating properties following injury of the peripheral and central nervous system. In this work, we studied PROG effects on glial cells of the normal and transected (TRX) spinal cord, to complement previous studies in motoneurons. Both neurons and glial cells expressed the classical PROG receptor (PR), suggesting that genomic mechanisms participated in PROG action. In TRX rats, PROG treatment stimulated the number of NADPH-diaphorase (nitric oxide synthase) active astrocytes, whereas the number of astrocytes expressing the glial fibrillary acidic protein (GFAP) was stimulated in control but not in TRX rats. PROG also stimulated the immunocytochemical staining for myelin-basic protein (MBP) and the number of oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2 in TRX rats. In terms of beneficial or detrimental consequences, these PROG effects may be supportive of neuronal recuperation, as shown for several neuronal functional parameters that were normalized by PROG treatment of spinal cord injured animals. Thus, PROG effects on glial cells go in parallel with morphological and biochemical evidence of survival of damaged motoneurons.
format	SER
author	De Nicola, A.F. Labombarda, F. Gonzalez, S.L. Gonzalez Deniselle, M.C. Guennoun, R. Schumacher, M.
author_facet	De Nicola, A.F. Labombarda, F. Gonzalez, S.L. Gonzalez Deniselle, M.C. Guennoun, R. Schumacher, M.
author_sort	De Nicola, A.F.
title	Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
title_short	Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
title_full	Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
title_fullStr	Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
title_full_unstemmed	Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?
title_sort	steroid effects on glial cells: detrimental or protective for spinal cord function?
url	http://hdl.handle.net/20.500.12110/paper_00778923_v1007_n_p317_DeNicola
work_keys_str_mv	AT denicolaaf steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction AT labombardaf steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction AT gonzalezsl steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction AT gonzalezdenisellemc steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction AT guennounr steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction AT schumacherm steroideffectsonglialcellsdetrimentalorprotectiveforspinalcordfunction
_version_	1807322697306210304

Steroid Effects on Glial Cells: Detrimental or Protective for Spinal Cord Function?

Ejemplares similares