Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis
The effect of chronic exposure to Escherichia coli on morphometrical parameters, different antioxidant defenses, lipid peroxidation and protein oxidation has been studied in digestive gland of the freshwater mussel Diplodon chilensis in the laboratory during 6 weeks. In, a parallel field study, we e...
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todo:paper_00759511_v41_n4_p356_Sabatini2023-10-03T14:54:03Z Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis Sabatini, S.E. Rocchetta, I. Luquet, C.M. Guido, M.I. de Molina, M.D.C.R. Antioxidant defenses Coliform bacteria Diplodon chilensis Oxidative stress Sewage pollution antioxidant coliform bacterium enzyme activity freshwater environment growth laboratory method mollusc morphometry oxidation pollution effect pollution exposure sewage Bacteria (microorganisms) Diplodon chilensis Escherichia coli The effect of chronic exposure to Escherichia coli on morphometrical parameters, different antioxidant defenses, lipid peroxidation and protein oxidation has been studied in digestive gland of the freshwater mussel Diplodon chilensis in the laboratory during 6 weeks. In, a parallel field study, we evaluated morphometrical and oxidative stress parameters in digestive glands of mussels collected from a sewage polluted area (SMA) and from a pristine area (control). Both sites were characterized by analyzing bacteriological and physic-chemical parameters. In the laboratory D. chilensis was able to filter bacteria at high concentrations with a clearing rate of 0.510 ± 0.036. L/h per gram of dry soft tissue mass. The digestive gland mass to shell length ratio (DGM/SL), reduced glutathione (GSH), lipid peroxidation, as concentration of thiobartituric acid reactive substances (TBARS), and glutathione-S-transferase (GST) activity of mussels fed with bacteria were significantly higher than those of control mussels after the fourth week. Fecal bacteria in lake water samples were undetectable in the control, and higher than 24,000. MPN (most probable number)/100. mL in SMA. DGM/SL was higher in SMA. No differences between sites were observed in total lipid and protein content, neither in superoxide dismutase activity. GSH content was higher in SMA, with no difference in the oxidized form. GST activity and MDA were significantly higher in SMA but protein oxidation was not affected. © 2011 Elsevier GmbH. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocchetta, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00759511_v41_n4_p356_Sabatini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant defenses Coliform bacteria Diplodon chilensis Oxidative stress Sewage pollution antioxidant coliform bacterium enzyme activity freshwater environment growth laboratory method mollusc morphometry oxidation pollution effect pollution exposure sewage Bacteria (microorganisms) Diplodon chilensis Escherichia coli |
| spellingShingle |
Antioxidant defenses Coliform bacteria Diplodon chilensis Oxidative stress Sewage pollution antioxidant coliform bacterium enzyme activity freshwater environment growth laboratory method mollusc morphometry oxidation pollution effect pollution exposure sewage Bacteria (microorganisms) Diplodon chilensis Escherichia coli Sabatini, S.E. Rocchetta, I. Luquet, C.M. Guido, M.I. de Molina, M.D.C.R. Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| topic_facet |
Antioxidant defenses Coliform bacteria Diplodon chilensis Oxidative stress Sewage pollution antioxidant coliform bacterium enzyme activity freshwater environment growth laboratory method mollusc morphometry oxidation pollution effect pollution exposure sewage Bacteria (microorganisms) Diplodon chilensis Escherichia coli |
| description |
The effect of chronic exposure to Escherichia coli on morphometrical parameters, different antioxidant defenses, lipid peroxidation and protein oxidation has been studied in digestive gland of the freshwater mussel Diplodon chilensis in the laboratory during 6 weeks. In, a parallel field study, we evaluated morphometrical and oxidative stress parameters in digestive glands of mussels collected from a sewage polluted area (SMA) and from a pristine area (control). Both sites were characterized by analyzing bacteriological and physic-chemical parameters. In the laboratory D. chilensis was able to filter bacteria at high concentrations with a clearing rate of 0.510 ± 0.036. L/h per gram of dry soft tissue mass. The digestive gland mass to shell length ratio (DGM/SL), reduced glutathione (GSH), lipid peroxidation, as concentration of thiobartituric acid reactive substances (TBARS), and glutathione-S-transferase (GST) activity of mussels fed with bacteria were significantly higher than those of control mussels after the fourth week. Fecal bacteria in lake water samples were undetectable in the control, and higher than 24,000. MPN (most probable number)/100. mL in SMA. DGM/SL was higher in SMA. No differences between sites were observed in total lipid and protein content, neither in superoxide dismutase activity. GSH content was higher in SMA, with no difference in the oxidized form. GST activity and MDA were significantly higher in SMA but protein oxidation was not affected. © 2011 Elsevier GmbH. |
| format |
JOUR |
| author |
Sabatini, S.E. Rocchetta, I. Luquet, C.M. Guido, M.I. de Molina, M.D.C.R. |
| author_facet |
Sabatini, S.E. Rocchetta, I. Luquet, C.M. Guido, M.I. de Molina, M.D.C.R. |
| author_sort |
Sabatini, S.E. |
| title |
Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| title_short |
Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| title_full |
Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| title_fullStr |
Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| title_full_unstemmed |
Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis |
| title_sort |
effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel diplodon chilensis |
| url |
http://hdl.handle.net/20.500.12110/paper_00759511_v41_n4_p356_Sabatini |
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