Mini-plasminogen like molecule in septic patients
Plasma from 7 septic patients with positive blood cultures were studied. None of them presented either clinical or laboratory evidence of Disseminated Intravascular Coagulation. The white cells count varied between 5 and 45×109/l. In plasma functional plasminogen levels varied between 25 and 45%, wh...
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todo:paper_00493848_v47_n5_p553_Kordich2023-10-03T14:52:40Z Mini-plasminogen like molecule in septic patients Kordich, L.C. Porterie, V.P. Lago, O. Bergonzelli, G.E. Sassetti, B. Sanchez Avalos, J.C. mini-plasminogen sepsis α2-antiplasmin alpha 2 antiplasmin elastase miniplasminogen unclassified drug article blood and hemopoietic system etiology human human cell leukocyte sepsis alpha 1-Antitrypsin Antiplasmin Blood Proteins Humans Leukocytes Pancreatic Elastase Plasminogen Sepsis Plasma from 7 septic patients with positive blood cultures were studied. None of them presented either clinical or laboratory evidence of Disseminated Intravascular Coagulation. The white cells count varied between 5 and 45×109/l. In plasma functional plasminogen levels varied between 25 and 45%, while those of α2-antiplasmin were normal (80-105%). The levels of elastase ranged between 250 and 750 ug/ml. Leukocyte elastase digests plasminogen "in vitro" and is able to produce several fragments; one of them called mini-plasminogen lacking lysine binding sites; therefore it does not bind to lysine-Sepharose 4B. Two different behaviors were observed in the plasmatic plasminogen of these patients with respect to their binding capacity to lysine-Sepharose 4 B. 3 patients had plasminogen which did not bind to lysine-Sepharose 4 B; the other 4 had two different components, one of which bound to lysine-Sepharose 4 B and another one which did not bind. Previous studies "in vitro" have shown that leukocyte elastase modifies α2-antiplasmin, initially producing a non-plasminogen binding form. A free α2-antiplasmin (non-plasminogen binding form) was detected in the plasma of these patients with sepsis by crossed immunoelectrophoresis with plasminogen in the first dimension. It seems tenable that high levels of leukocyte elastase could be responsible for these findings although, the possible relationships to leukocyte elastase still remain to be proven but could possibly explain this effect. © 1987. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00493848_v47_n5_p553_Kordich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
mini-plasminogen sepsis α2-antiplasmin alpha 2 antiplasmin elastase miniplasminogen unclassified drug article blood and hemopoietic system etiology human human cell leukocyte sepsis alpha 1-Antitrypsin Antiplasmin Blood Proteins Humans Leukocytes Pancreatic Elastase Plasminogen Sepsis |
| spellingShingle |
mini-plasminogen sepsis α2-antiplasmin alpha 2 antiplasmin elastase miniplasminogen unclassified drug article blood and hemopoietic system etiology human human cell leukocyte sepsis alpha 1-Antitrypsin Antiplasmin Blood Proteins Humans Leukocytes Pancreatic Elastase Plasminogen Sepsis Kordich, L.C. Porterie, V.P. Lago, O. Bergonzelli, G.E. Sassetti, B. Sanchez Avalos, J.C. Mini-plasminogen like molecule in septic patients |
| topic_facet |
mini-plasminogen sepsis α2-antiplasmin alpha 2 antiplasmin elastase miniplasminogen unclassified drug article blood and hemopoietic system etiology human human cell leukocyte sepsis alpha 1-Antitrypsin Antiplasmin Blood Proteins Humans Leukocytes Pancreatic Elastase Plasminogen Sepsis |
| description |
Plasma from 7 septic patients with positive blood cultures were studied. None of them presented either clinical or laboratory evidence of Disseminated Intravascular Coagulation. The white cells count varied between 5 and 45×109/l. In plasma functional plasminogen levels varied between 25 and 45%, while those of α2-antiplasmin were normal (80-105%). The levels of elastase ranged between 250 and 750 ug/ml. Leukocyte elastase digests plasminogen "in vitro" and is able to produce several fragments; one of them called mini-plasminogen lacking lysine binding sites; therefore it does not bind to lysine-Sepharose 4B. Two different behaviors were observed in the plasmatic plasminogen of these patients with respect to their binding capacity to lysine-Sepharose 4 B. 3 patients had plasminogen which did not bind to lysine-Sepharose 4 B; the other 4 had two different components, one of which bound to lysine-Sepharose 4 B and another one which did not bind. Previous studies "in vitro" have shown that leukocyte elastase modifies α2-antiplasmin, initially producing a non-plasminogen binding form. A free α2-antiplasmin (non-plasminogen binding form) was detected in the plasma of these patients with sepsis by crossed immunoelectrophoresis with plasminogen in the first dimension. It seems tenable that high levels of leukocyte elastase could be responsible for these findings although, the possible relationships to leukocyte elastase still remain to be proven but could possibly explain this effect. © 1987. |
| format |
JOUR |
| author |
Kordich, L.C. Porterie, V.P. Lago, O. Bergonzelli, G.E. Sassetti, B. Sanchez Avalos, J.C. |
| author_facet |
Kordich, L.C. Porterie, V.P. Lago, O. Bergonzelli, G.E. Sassetti, B. Sanchez Avalos, J.C. |
| author_sort |
Kordich, L.C. |
| title |
Mini-plasminogen like molecule in septic patients |
| title_short |
Mini-plasminogen like molecule in septic patients |
| title_full |
Mini-plasminogen like molecule in septic patients |
| title_fullStr |
Mini-plasminogen like molecule in septic patients |
| title_full_unstemmed |
Mini-plasminogen like molecule in septic patients |
| title_sort |
mini-plasminogen like molecule in septic patients |
| url |
http://hdl.handle.net/20.500.12110/paper_00493848_v47_n5_p553_Kordich |
| work_keys_str_mv |
AT kordichlc miniplasminogenlikemoleculeinsepticpatients AT porterievp miniplasminogenlikemoleculeinsepticpatients AT lagoo miniplasminogenlikemoleculeinsepticpatients AT bergonzellige miniplasminogenlikemoleculeinsepticpatients AT sassettib miniplasminogenlikemoleculeinsepticpatients AT sanchezavalosjc miniplasminogenlikemoleculeinsepticpatients |
| _version_ |
1782027856509927424 |