Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos

Environmental contaminants produce multiple adverse consequences at individual, population and ecosystem levels. High volumes of agrochemicals applied to great variety of crops, together with agricultural expansion, generate great concerns due to the impact for the environment and large risk implica...

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Autores principales:	Schaumburg, L.G., Siroski, P.A., Poletta, G.L., Mudry, M.D.
Formato:	JOUR
Materias:	Eggs Genotoxic effects Glyphosate-based formulation Tegu lizard glycine glyphosate herbicide analogs and derivatives animal comet assay dose response drug effects embryo development growth, development and aging lizard micronucleus test nonmammalian embryo Animals Comet Assay Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Glycine Herbicides Lizards Micronucleus Tests
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00483575_v130_n_p71_Schaumburg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00483575_v130_n_p71_Schaumburg
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spelling	todo:paper_00483575_v130_n_p71_Schaumburg2023-10-03T14:52:27Z Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos Schaumburg, L.G. Siroski, P.A. Poletta, G.L. Mudry, M.D. Eggs Genotoxic effects Glyphosate-based formulation Tegu lizard glycine glyphosate herbicide analogs and derivatives animal comet assay dose response drug effects embryo development growth, development and aging lizard micronucleus test nonmammalian embryo Animals Comet Assay Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Glycine Herbicides Lizards Micronucleus Tests Environmental contaminants produce multiple adverse consequences at individual, population and ecosystem levels. High volumes of agrochemicals applied to great variety of crops, together with agricultural expansion, generate great concerns due to the impact for the environment and large risk implicated for wildlife. The lack of data on these threats is striking. The tegu lizard (Salvator merianae) is one of the species that live in environments under contaminant effects. Several characteristics allow proposing this species as a potential sentinel organism for the monitoring of pesticides in their habitat. The present study is the first report about genotoxicity in tegu lizard neonates after embryonic exposure to Roundup® (glyphosate 66.2%). The micronucleus test (MN), nuclear abnormalities (NAs) assay and comet assay (CA) were used as biomarkers of genotoxic effects induced in erythrocytes by topical exposure of the eggs to the glyphosate commercial formulation Roundup® (RU), in laboratory controlled conditions. A total of 96 eggs were distributed in six groups exposed to RU (50, 100, 200, 400, 800, 1600 μg/egg), one positive control (PC; 200 μg cyclophosphamide/egg) and one negative control (NC; distilled water). No teratogenic effects were observed in any of the exposed or control neonates. A significant increase in DNA damage was observed in all concentrations higher than 100 μg/egg with respect to NC (p < 0.05). However, no statistical differences were found in the frequencies of MN and NAs in any group exposed to RU compared to the NC. No statistically significant differences were found in the size of the lizards at birth or after six months post-exposure (p > 0.05). Our results provide new information about the undesirable effects of the glyphosate-based herbicide formulations RU on this lizard species that inhabits areas permanently exposed to several pesticide formulations. We consider of utmost necessity a strict regulation of the agrochemical application conditions in those environments near to places where wild populations of terrestrial and aquatic species live, in order to minimize the adverse effects on ecosystems. © 2015 Elsevier B.V. Fil:Poletta, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00483575_v130_n_p71_Schaumburg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Eggs Genotoxic effects Glyphosate-based formulation Tegu lizard glycine glyphosate herbicide analogs and derivatives animal comet assay dose response drug effects embryo development growth, development and aging lizard micronucleus test nonmammalian embryo Animals Comet Assay Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Glycine Herbicides Lizards Micronucleus Tests
spellingShingle	Eggs Genotoxic effects Glyphosate-based formulation Tegu lizard glycine glyphosate herbicide analogs and derivatives animal comet assay dose response drug effects embryo development growth, development and aging lizard micronucleus test nonmammalian embryo Animals Comet Assay Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Glycine Herbicides Lizards Micronucleus Tests Schaumburg, L.G. Siroski, P.A. Poletta, G.L. Mudry, M.D. Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
topic_facet	Eggs Genotoxic effects Glyphosate-based formulation Tegu lizard glycine glyphosate herbicide analogs and derivatives animal comet assay dose response drug effects embryo development growth, development and aging lizard micronucleus test nonmammalian embryo Animals Comet Assay Dose-Response Relationship, Drug Embryo, Nonmammalian Embryonic Development Glycine Herbicides Lizards Micronucleus Tests
description	Environmental contaminants produce multiple adverse consequences at individual, population and ecosystem levels. High volumes of agrochemicals applied to great variety of crops, together with agricultural expansion, generate great concerns due to the impact for the environment and large risk implicated for wildlife. The lack of data on these threats is striking. The tegu lizard (Salvator merianae) is one of the species that live in environments under contaminant effects. Several characteristics allow proposing this species as a potential sentinel organism for the monitoring of pesticides in their habitat. The present study is the first report about genotoxicity in tegu lizard neonates after embryonic exposure to Roundup® (glyphosate 66.2%). The micronucleus test (MN), nuclear abnormalities (NAs) assay and comet assay (CA) were used as biomarkers of genotoxic effects induced in erythrocytes by topical exposure of the eggs to the glyphosate commercial formulation Roundup® (RU), in laboratory controlled conditions. A total of 96 eggs were distributed in six groups exposed to RU (50, 100, 200, 400, 800, 1600 μg/egg), one positive control (PC; 200 μg cyclophosphamide/egg) and one negative control (NC; distilled water). No teratogenic effects were observed in any of the exposed or control neonates. A significant increase in DNA damage was observed in all concentrations higher than 100 μg/egg with respect to NC (p < 0.05). However, no statistical differences were found in the frequencies of MN and NAs in any group exposed to RU compared to the NC. No statistically significant differences were found in the size of the lizards at birth or after six months post-exposure (p > 0.05). Our results provide new information about the undesirable effects of the glyphosate-based herbicide formulations RU on this lizard species that inhabits areas permanently exposed to several pesticide formulations. We consider of utmost necessity a strict regulation of the agrochemical application conditions in those environments near to places where wild populations of terrestrial and aquatic species live, in order to minimize the adverse effects on ecosystems. © 2015 Elsevier B.V.
format	JOUR
author	Schaumburg, L.G. Siroski, P.A. Poletta, G.L. Mudry, M.D.
author_facet	Schaumburg, L.G. Siroski, P.A. Poletta, G.L. Mudry, M.D.
author_sort	Schaumburg, L.G.
title	Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
title_short	Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
title_full	Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
title_fullStr	Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
title_full_unstemmed	Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
title_sort	genotoxicity induced by roundup® (glyphosate) in tegu lizard (salvator merianae) embryos
url	http://hdl.handle.net/20.500.12110/paper_00483575_v130_n_p71_Schaumburg
work_keys_str_mv	AT schaumburglg genotoxicityinducedbyroundupglyphosateintegulizardsalvatormerianaeembryos AT siroskipa genotoxicityinducedbyroundupglyphosateintegulizardsalvatormerianaeembryos AT polettagl genotoxicityinducedbyroundupglyphosateintegulizardsalvatormerianaeembryos AT mudrymd genotoxicityinducedbyroundupglyphosateintegulizardsalvatormerianaeembryos
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Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos

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