Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis

Bulnesia, a genus of the Zygophyllaceae, comprises two subgenera, subgenus Bulnesia and subgenus Gonopterodendron. One species of each subgenus was studied: B. schickendantzii and B. bonariensis, respectively. B. schickendantzii endosperm consists of four to six cell layers. Cell walls are composed...

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Autores principales: Maldonado, S., Lima, C., Etchart, M., Láinez, V., De Lederkremer, R.M.
Formato: JOUR
Materias:
Bulnesia
Carbohydrates
Seed proteins
Seed ultrastructure
Zygophyllaceae
Bulnesia bonariensis
Bulnesia shickendantzii
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00445983_v47_n3_p285_Maldonado
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00445983_v47_n3_p285_Maldonado
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spelling todo:paper_00445983_v47_n3_p285_Maldonado2023-10-03T14:51:43Z Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis Maldonado, S. Lima, C. Etchart, M. Láinez, V. De Lederkremer, R.M. Bulnesia Carbohydrates Seed proteins Seed ultrastructure Zygophyllaceae Bulnesia bonariensis Bulnesia shickendantzii Bulnesia, a genus of the Zygophyllaceae, comprises two subgenera, subgenus Bulnesia and subgenus Gonopterodendron. One species of each subgenus was studied: B. schickendantzii and B. bonariensis, respectively. B. schickendantzii endosperm consists of four to six cell layers. Cell walls are composed of a middle lamella; a very thick outer wall and a thin inner wall. B. bonariensis endosperm is reduced to one layer of cells. The cell walls are formed by a middle lamella and a thinner wall. Protein bodies are observed in the embryo of both species and in the endosperm of B. schickendantzii. Lipid vesicles are present in the embryo and endosperm of both species. Phytin crystals, present in the protein bodies of B. schickendantzii, are rare in the protein bodies of B. bonariensis. B. schickendantzii endosperm contains the majority of sugars and proteins. In B. bonariensis the higher percentage of carbohydrate and protein is found in the embryo. Determination of neutral sugars was performed after acid hydrolysis. Sugars were analysed as alditol acetates by gas-liquid chromatography. Arabinose, xylose, galactose and glucose are the main monosaccharides but the ratio differs in tissues of both species. Soluble proteins were analysed by polyacrylamide gel electrophoresis, showing a different profile in tissues of both species. Very few bands are glycosylated. The ultrastructural and chemical differences observed between both species suggest the segregation of Gonopterodendron from the genus Bulnesia as a separate genus and encourage further studies of the other six species in order to confirm this assertion. Fil:Láinez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00445983_v47_n3_p285_Maldonado
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bulnesia
Carbohydrates
Seed proteins
Seed ultrastructure
Zygophyllaceae
Bulnesia bonariensis
Bulnesia shickendantzii
spellingShingle Bulnesia
Carbohydrates
Seed proteins
Seed ultrastructure
Zygophyllaceae
Bulnesia bonariensis
Bulnesia shickendantzii
Maldonado, S.
Lima, C.
Etchart, M.
Láinez, V.
De Lederkremer, R.M.
Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
topic_facet Bulnesia
Carbohydrates
Seed proteins
Seed ultrastructure
Zygophyllaceae
Bulnesia bonariensis
Bulnesia shickendantzii
description Bulnesia, a genus of the Zygophyllaceae, comprises two subgenera, subgenus Bulnesia and subgenus Gonopterodendron. One species of each subgenus was studied: B. schickendantzii and B. bonariensis, respectively. B. schickendantzii endosperm consists of four to six cell layers. Cell walls are composed of a middle lamella; a very thick outer wall and a thin inner wall. B. bonariensis endosperm is reduced to one layer of cells. The cell walls are formed by a middle lamella and a thinner wall. Protein bodies are observed in the embryo of both species and in the endosperm of B. schickendantzii. Lipid vesicles are present in the embryo and endosperm of both species. Phytin crystals, present in the protein bodies of B. schickendantzii, are rare in the protein bodies of B. bonariensis. B. schickendantzii endosperm contains the majority of sugars and proteins. In B. bonariensis the higher percentage of carbohydrate and protein is found in the embryo. Determination of neutral sugars was performed after acid hydrolysis. Sugars were analysed as alditol acetates by gas-liquid chromatography. Arabinose, xylose, galactose and glucose are the main monosaccharides but the ratio differs in tissues of both species. Soluble proteins were analysed by polyacrylamide gel electrophoresis, showing a different profile in tissues of both species. Very few bands are glycosylated. The ultrastructural and chemical differences observed between both species suggest the segregation of Gonopterodendron from the genus Bulnesia as a separate genus and encourage further studies of the other six species in order to confirm this assertion.
format JOUR
author Maldonado, S.
Lima, C.
Etchart, M.
Láinez, V.
De Lederkremer, R.M.
author_facet Maldonado, S.
Lima, C.
Etchart, M.
Láinez, V.
De Lederkremer, R.M.
author_sort Maldonado, S.
title Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
title_short Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
title_full Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
title_fullStr Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
title_full_unstemmed Ultrastructural and chemical studies on seeds of Bulnesia schickendantzii and Bulnesia bonariensis
title_sort ultrastructural and chemical studies on seeds of bulnesia schickendantzii and bulnesia bonariensis
url http://hdl.handle.net/20.500.12110/paper_00445983_v47_n3_p285_Maldonado
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AT limac ultrastructuralandchemicalstudiesonseedsofbulnesiaschickendantziiandbulnesiabonariensis
AT etchartm ultrastructuralandchemicalstudiesonseedsofbulnesiaschickendantziiandbulnesiabonariensis
AT lainezv ultrastructuralandchemicalstudiesonseedsofbulnesiaschickendantziiandbulnesiabonariensis
AT delederkremerrm ultrastructuralandchemicalstudiesonseedsofbulnesiaschickendantziiandbulnesiabonariensis
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