Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto |
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todo:paper_00399140_v85_n1_p455_Bonetto2023-10-03T14:50:02Z Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay Bonetto, M.C. Sacco, N.J. Ohlsson, A.H. Cortón, E. Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved. Fil:Bonetto, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sacco, N.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay |
| spellingShingle |
Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay Bonetto, M.C. Sacco, N.J. Ohlsson, A.H. Cortón, E. Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| topic_facet |
Amperometric Bioassay BODSEED Ferricyanide Formaldehyde Klebsiella pneumoniae Amino acids Bacteria Formaldehyde Oxygen Amperometric Amperometric detection BODSEED Environmental strain Ferricyanide Klebsiella pneumoniae Long-term incubation Municipal wastewaters Bioassay |
| description |
Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Bonetto, M.C. Sacco, N.J. Ohlsson, A.H. Cortón, E. |
| author_facet |
Bonetto, M.C. Sacco, N.J. Ohlsson, A.H. Cortón, E. |
| author_sort |
Bonetto, M.C. |
| title |
Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| title_short |
Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| title_full |
Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| title_fullStr |
Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| title_full_unstemmed |
Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
| title_sort |
assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated bod assay |
| url |
http://hdl.handle.net/20.500.12110/paper_00399140_v85_n1_p455_Bonetto |
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| _version_ |
1782030748857925632 |