Detection and quantification of reactive oxygen species (ROS) in indoor air

Reactive oxygen species (ROS), such as free radicals and peroxides, are environmental trace pollutants potentially associated with asthma and airways inflammation. These compounds are often not detected in indoor air due to sampling and analytical limitations. This study developed and validated an e...

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Autores principales:	Montesinos, V.N., Sleiman, M., Cohn, S., Litter, M.I., Destaillats, H.
Formato:	JOUR
Materias:	Hydrogen peroxide Hydroxyl radical Plasma air cleaner Reactive oxygen species (ROS) Air quality Byproducts Efficiency Fluorescence Free radicals Indoor air pollution Oxidation Ozone Peroxides Probes Detection and quantifications Experimental methods High collection efficiency Hydroxyl radicals Low detection limit Quantification limit Reactive oxygen species Simultaneous determinations Air cleaners 2',7'-dichlorofluorescein 2-hydroxyterephthalic acid fluorescein derivative fluorescent dye hydrogen peroxide hydroxyl radical ozone phthalic acid derivative reactive oxygen metabolite analysis chemistry indoor air pollution limit of detection Air Pollution, Indoor Fluoresceins Fluorescent Dyes Hydrogen Peroxide Hydroxyl Radical Limit of Detection Phthalic Acids Reactive Oxygen Species
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00399140_v138_n_p20_Montesinos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00399140_v138_n_p20_Montesinos2023-10-03T14:49:58Z Detection and quantification of reactive oxygen species (ROS) in indoor air Montesinos, V.N. Sleiman, M. Cohn, S. Litter, M.I. Destaillats, H. Hydrogen peroxide Hydroxyl radical Plasma air cleaner Reactive oxygen species (ROS) Air quality Byproducts Efficiency Fluorescence Free radicals Hydrogen peroxide Indoor air pollution Oxidation Ozone Peroxides Probes Detection and quantifications Experimental methods High collection efficiency Hydroxyl radicals Low detection limit Quantification limit Reactive oxygen species Simultaneous determinations Air cleaners 2',7'-dichlorofluorescein 2-hydroxyterephthalic acid fluorescein derivative fluorescent dye hydrogen peroxide hydroxyl radical ozone phthalic acid derivative reactive oxygen metabolite analysis chemistry indoor air pollution limit of detection Air Pollution, Indoor Fluoresceins Fluorescent Dyes Hydrogen Peroxide Hydroxyl Radical Limit of Detection Ozone Phthalic Acids Reactive Oxygen Species Reactive oxygen species (ROS), such as free radicals and peroxides, are environmental trace pollutants potentially associated with asthma and airways inflammation. These compounds are often not detected in indoor air due to sampling and analytical limitations. This study developed and validated an experimental method to sample, identify and quantify ROS in indoor air using fluorescent probes. Tests were carried out simultaneously using three different probes: 2′,7′-dichlorofluorescin (DCFH) to detect a broad range of ROS, Amplex ultra Red® (AuR) to detect peroxides, and terephthalic acid (TPA) to detect hydroxyl radicals (HO•). For each test, air samples were collected using two impingers in series kept in an ice bath, containing each 10 mL of 50 mM phosphate buffer at pH 7.2. In tests with TPA, that probe was also added to the buffer prior to sampling; in the other two tests, probes and additional reactants were added immediately after sampling. The concentration of fluorescent byproducts was determined fluorometrically. Calibration curves were developed by reacting DCFH and AuR with known amounts of H2O2, and using known amounts of 2-hydroxyterephthalic acid (HTPA) for TPA. Low detection limits (9-13 nM) and quantification limits (18-22 nM) were determined for all three probes, which presented a linear response in the range 10-500 nM for AuR and TPA, and 100-2000 nM for DCFH. High collection efficiency (CE) and recovery efficiency (RE) were observed for DCFH (CE=RE=100%) and AuR (CE=100%; RE=73%) by sampling from a laboratory-developed gas phase H2O2 generator. Interference of co-occurring ozone was evaluated and quantified for the three probes by sampling from the outlet of an ozone generator. The method was demonstrated by sampling air emitted by two portable air cleaners: a strong ozone generator (AC1) and a plasma generator (AC2). High ozone levels emitted by AC1 did not allow for simultaneous determination of ROS levels due to high background levels associated with ozone decomposition in the buffer. However, emitted ROS were quantified at the outlet of AC2 using two of the three probes. With AuR, the concentration of peroxides in air emitted by the air cleaner was 300 ppt of H2O2 equivalents. With TPA, the HO• concentration was 47 ppt. This method is best suited to quantify ROS in the presence of low ozone levels. © 2015, Elsevier B.V. All rights reserved. Fil:Litter, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Destaillats, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00399140_v138_n_p20_Montesinos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Hydrogen peroxide Hydroxyl radical Plasma air cleaner Reactive oxygen species (ROS) Air quality Byproducts Efficiency Fluorescence Free radicals Hydrogen peroxide Indoor air pollution Oxidation Ozone Peroxides Probes Detection and quantifications Experimental methods High collection efficiency Hydroxyl radicals Low detection limit Quantification limit Reactive oxygen species Simultaneous determinations Air cleaners 2',7'-dichlorofluorescein 2-hydroxyterephthalic acid fluorescein derivative fluorescent dye hydrogen peroxide hydroxyl radical ozone phthalic acid derivative reactive oxygen metabolite analysis chemistry indoor air pollution limit of detection Air Pollution, Indoor Fluoresceins Fluorescent Dyes Hydrogen Peroxide Hydroxyl Radical Limit of Detection Ozone Phthalic Acids Reactive Oxygen Species
spellingShingle	Hydrogen peroxide Hydroxyl radical Plasma air cleaner Reactive oxygen species (ROS) Air quality Byproducts Efficiency Fluorescence Free radicals Hydrogen peroxide Indoor air pollution Oxidation Ozone Peroxides Probes Detection and quantifications Experimental methods High collection efficiency Hydroxyl radicals Low detection limit Quantification limit Reactive oxygen species Simultaneous determinations Air cleaners 2',7'-dichlorofluorescein 2-hydroxyterephthalic acid fluorescein derivative fluorescent dye hydrogen peroxide hydroxyl radical ozone phthalic acid derivative reactive oxygen metabolite analysis chemistry indoor air pollution limit of detection Air Pollution, Indoor Fluoresceins Fluorescent Dyes Hydrogen Peroxide Hydroxyl Radical Limit of Detection Ozone Phthalic Acids Reactive Oxygen Species Montesinos, V.N. Sleiman, M. Cohn, S. Litter, M.I. Destaillats, H. Detection and quantification of reactive oxygen species (ROS) in indoor air
topic_facet	Hydrogen peroxide Hydroxyl radical Plasma air cleaner Reactive oxygen species (ROS) Air quality Byproducts Efficiency Fluorescence Free radicals Hydrogen peroxide Indoor air pollution Oxidation Ozone Peroxides Probes Detection and quantifications Experimental methods High collection efficiency Hydroxyl radicals Low detection limit Quantification limit Reactive oxygen species Simultaneous determinations Air cleaners 2',7'-dichlorofluorescein 2-hydroxyterephthalic acid fluorescein derivative fluorescent dye hydrogen peroxide hydroxyl radical ozone phthalic acid derivative reactive oxygen metabolite analysis chemistry indoor air pollution limit of detection Air Pollution, Indoor Fluoresceins Fluorescent Dyes Hydrogen Peroxide Hydroxyl Radical Limit of Detection Ozone Phthalic Acids Reactive Oxygen Species
description	Reactive oxygen species (ROS), such as free radicals and peroxides, are environmental trace pollutants potentially associated with asthma and airways inflammation. These compounds are often not detected in indoor air due to sampling and analytical limitations. This study developed and validated an experimental method to sample, identify and quantify ROS in indoor air using fluorescent probes. Tests were carried out simultaneously using three different probes: 2′,7′-dichlorofluorescin (DCFH) to detect a broad range of ROS, Amplex ultra Red® (AuR) to detect peroxides, and terephthalic acid (TPA) to detect hydroxyl radicals (HO•). For each test, air samples were collected using two impingers in series kept in an ice bath, containing each 10 mL of 50 mM phosphate buffer at pH 7.2. In tests with TPA, that probe was also added to the buffer prior to sampling; in the other two tests, probes and additional reactants were added immediately after sampling. The concentration of fluorescent byproducts was determined fluorometrically. Calibration curves were developed by reacting DCFH and AuR with known amounts of H2O2, and using known amounts of 2-hydroxyterephthalic acid (HTPA) for TPA. Low detection limits (9-13 nM) and quantification limits (18-22 nM) were determined for all three probes, which presented a linear response in the range 10-500 nM for AuR and TPA, and 100-2000 nM for DCFH. High collection efficiency (CE) and recovery efficiency (RE) were observed for DCFH (CE=RE=100%) and AuR (CE=100%; RE=73%) by sampling from a laboratory-developed gas phase H2O2 generator. Interference of co-occurring ozone was evaluated and quantified for the three probes by sampling from the outlet of an ozone generator. The method was demonstrated by sampling air emitted by two portable air cleaners: a strong ozone generator (AC1) and a plasma generator (AC2). High ozone levels emitted by AC1 did not allow for simultaneous determination of ROS levels due to high background levels associated with ozone decomposition in the buffer. However, emitted ROS were quantified at the outlet of AC2 using two of the three probes. With AuR, the concentration of peroxides in air emitted by the air cleaner was 300 ppt of H2O2 equivalents. With TPA, the HO• concentration was 47 ppt. This method is best suited to quantify ROS in the presence of low ozone levels. © 2015, Elsevier B.V. All rights reserved.
format	JOUR
author	Montesinos, V.N. Sleiman, M. Cohn, S. Litter, M.I. Destaillats, H.
author_facet	Montesinos, V.N. Sleiman, M. Cohn, S. Litter, M.I. Destaillats, H.
author_sort	Montesinos, V.N.
title	Detection and quantification of reactive oxygen species (ROS) in indoor air
title_short	Detection and quantification of reactive oxygen species (ROS) in indoor air
title_full	Detection and quantification of reactive oxygen species (ROS) in indoor air
title_fullStr	Detection and quantification of reactive oxygen species (ROS) in indoor air
title_full_unstemmed	Detection and quantification of reactive oxygen species (ROS) in indoor air
title_sort	detection and quantification of reactive oxygen species (ros) in indoor air
url	http://hdl.handle.net/20.500.12110/paper_00399140_v138_n_p20_Montesinos
work_keys_str_mv	AT montesinosvn detectionandquantificationofreactiveoxygenspeciesrosinindoorair AT sleimanm detectionandquantificationofreactiveoxygenspeciesrosinindoorair AT cohns detectionandquantificationofreactiveoxygenspeciesrosinindoorair AT littermi detectionandquantificationofreactiveoxygenspeciesrosinindoorair AT destaillatsh detectionandquantificationofreactiveoxygenspeciesrosinindoorair
_version_	1807321979848491008

Detection and quantification of reactive oxygen species (ROS) in indoor air

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