Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone
11β-hydroxyprogesterone (HOP) and 11-ketoprogesterone (KP) are reversible components of a shuttle pair whose interconversion in rat liver is catalyzed by isoform-1 of 11β-hydroxysteroid dehydrogenase. Kidneys also produce this interconversion. The present study was carried out to investigate the shu...
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todo:paper_0039128X_v62_n4_p358_Galigniana2023-10-03T14:49:25Z Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone Galigniana, M.D. Vicent, G.P. Burton, G. Lantos, C.P. 11β-hydroxyprogesterone 11β-hydroxysteroid dehydrogenase 11-ketoprogesterone shuttle pair sodium retention 11beta hydroxyprogesterone 11beta hydroxysteroid dehydrogenase 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid corticosterone dehydrocorticosterone mineralocorticoid receptor animal cell animal experiment animal model animal tissue article enzyme activity male nonhuman oxidation ph rat receptor binding shuttle vector sodium retention tissue specificity 11-beta-Hydroxysteroid Dehydrogenases Adrenalectomy Animals Binding, Competitive Cholic Acids Enzyme Induction Hydrogen-Ion Concentration Hydroxyprogesterones Hydroxysteroid Dehydrogenases Male Progesterone Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Transcortin Tyrosine Transaminase Animalia Humulus 11β-hydroxyprogesterone (HOP) and 11-ketoprogesterone (KP) are reversible components of a shuttle pair whose interconversion in rat liver is catalyzed by isoform-1 of 11β-hydroxysteroid dehydrogenase. Kidneys also produce this interconversion. The present study was carried out to investigate the shuttle pair and its components in the rat. As in corticosterone/11 -dehydrocorticosterone, oxidation is more effective at an alkaline pH, while reduction prevails at a neutral pH. Moreover, both reactions are inhibited by the detergent 3-[(3-cholamido propyl)- dimethylammonio]-1-propane-sulphonate (CHAPS). However, at variance with the 11-ketosteroids cortisone (E) and 11-dehydrocorticosterone (A) thought to be 'inactive,' KP has slight direct Na+-retaining properties, and it, as well as HOP, induces glucocorticoids (11β-hydroxycorticoids) to retain sodium. 11-ketoprogesterone exhibits 17 times better affinity for native type I mineralocorticoid receptor than HOP and a 3-fold affinity for partially purified (transcortin free) mineralocorticoid receptor. However, KP, in contrast to HOP, binds only weakly to transcortin, not at all to glucocorticoid receptor, and requires reduction at C11 for tyrosine aminotransferase (TAT) induction. Fil:Vicent, G.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burton, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0039128X_v62_n4_p358_Galigniana |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
11β-hydroxyprogesterone 11β-hydroxysteroid dehydrogenase 11-ketoprogesterone shuttle pair sodium retention 11beta hydroxyprogesterone 11beta hydroxysteroid dehydrogenase 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid corticosterone dehydrocorticosterone mineralocorticoid receptor animal cell animal experiment animal model animal tissue article enzyme activity male nonhuman oxidation ph rat receptor binding shuttle vector sodium retention tissue specificity 11-beta-Hydroxysteroid Dehydrogenases Adrenalectomy Animals Binding, Competitive Cholic Acids Enzyme Induction Hydrogen-Ion Concentration Hydroxyprogesterones Hydroxysteroid Dehydrogenases Male Progesterone Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Transcortin Tyrosine Transaminase Animalia Humulus |
| spellingShingle |
11β-hydroxyprogesterone 11β-hydroxysteroid dehydrogenase 11-ketoprogesterone shuttle pair sodium retention 11beta hydroxyprogesterone 11beta hydroxysteroid dehydrogenase 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid corticosterone dehydrocorticosterone mineralocorticoid receptor animal cell animal experiment animal model animal tissue article enzyme activity male nonhuman oxidation ph rat receptor binding shuttle vector sodium retention tissue specificity 11-beta-Hydroxysteroid Dehydrogenases Adrenalectomy Animals Binding, Competitive Cholic Acids Enzyme Induction Hydrogen-Ion Concentration Hydroxyprogesterones Hydroxysteroid Dehydrogenases Male Progesterone Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Transcortin Tyrosine Transaminase Animalia Humulus Galigniana, M.D. Vicent, G.P. Burton, G. Lantos, C.P. Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| topic_facet |
11β-hydroxyprogesterone 11β-hydroxysteroid dehydrogenase 11-ketoprogesterone shuttle pair sodium retention 11beta hydroxyprogesterone 11beta hydroxysteroid dehydrogenase 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid corticosterone dehydrocorticosterone mineralocorticoid receptor animal cell animal experiment animal model animal tissue article enzyme activity male nonhuman oxidation ph rat receptor binding shuttle vector sodium retention tissue specificity 11-beta-Hydroxysteroid Dehydrogenases Adrenalectomy Animals Binding, Competitive Cholic Acids Enzyme Induction Hydrogen-Ion Concentration Hydroxyprogesterones Hydroxysteroid Dehydrogenases Male Progesterone Rats Rats, Sprague-Dawley Receptors, Glucocorticoid Receptors, Mineralocorticoid Sodium Transcortin Tyrosine Transaminase Animalia Humulus |
| description |
11β-hydroxyprogesterone (HOP) and 11-ketoprogesterone (KP) are reversible components of a shuttle pair whose interconversion in rat liver is catalyzed by isoform-1 of 11β-hydroxysteroid dehydrogenase. Kidneys also produce this interconversion. The present study was carried out to investigate the shuttle pair and its components in the rat. As in corticosterone/11 -dehydrocorticosterone, oxidation is more effective at an alkaline pH, while reduction prevails at a neutral pH. Moreover, both reactions are inhibited by the detergent 3-[(3-cholamido propyl)- dimethylammonio]-1-propane-sulphonate (CHAPS). However, at variance with the 11-ketosteroids cortisone (E) and 11-dehydrocorticosterone (A) thought to be 'inactive,' KP has slight direct Na+-retaining properties, and it, as well as HOP, induces glucocorticoids (11β-hydroxycorticoids) to retain sodium. 11-ketoprogesterone exhibits 17 times better affinity for native type I mineralocorticoid receptor than HOP and a 3-fold affinity for partially purified (transcortin free) mineralocorticoid receptor. However, KP, in contrast to HOP, binds only weakly to transcortin, not at all to glucocorticoid receptor, and requires reduction at C11 for tyrosine aminotransferase (TAT) induction. |
| format |
JOUR |
| author |
Galigniana, M.D. Vicent, G.P. Burton, G. Lantos, C.P. |
| author_facet |
Galigniana, M.D. Vicent, G.P. Burton, G. Lantos, C.P. |
| author_sort |
Galigniana, M.D. |
| title |
Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| title_short |
Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| title_full |
Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| title_fullStr |
Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| title_full_unstemmed |
Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| title_sort |
features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone |
| url |
http://hdl.handle.net/20.500.12110/paper_0039128X_v62_n4_p358_Galigniana |
| work_keys_str_mv |
AT galignianamd featuresoftheshuttlepair11bhydroxyprogesterone11ketoprogesterone AT vicentgp featuresoftheshuttlepair11bhydroxyprogesterone11ketoprogesterone AT burtong featuresoftheshuttlepair11bhydroxyprogesterone11ketoprogesterone AT lantoscp featuresoftheshuttlepair11bhydroxyprogesterone11ketoprogesterone |
| _version_ |
1782027951134474240 |