A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to...
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todo:paper_0039128X_v102_n_p46_Tossi2023-10-03T14:49:17Z A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production Tossi, V.E. Acebedo, S.L. Cassia, R.O. Lamattina, L. Galagovsky, L.R. Ramírez, J.A. Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies. © 2015 Elsevier Inc. Fil:Acebedo, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramírez, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0039128X_v102_n_p46_Tossi |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays |
| spellingShingle |
Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays Tossi, V.E. Acebedo, S.L. Cassia, R.O. Lamattina, L. Galagovsky, L.R. Ramírez, J.A. A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| topic_facet |
Bioactivity assay Brassinosteroids Nitric oxide brassinosteroid nitric oxide brassinolide brassinosteroid heterocyclic steroid nitric oxide analytic method Article bioassay controlled study fluorometry in vitro study intermethod comparison maize mesophyll cell nonhuman plant leaf tomato bioassay cytology fluorometry metabolism plant cell procedures Lycopersicon esculentum Zea mays Biological Assay Brassinosteroids Fluorometry Nitric Oxide Plant Cells Plant Leaves Steroids, Heterocyclic Zea mays |
| description |
Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies. © 2015 Elsevier Inc. |
| format |
JOUR |
| author |
Tossi, V.E. Acebedo, S.L. Cassia, R.O. Lamattina, L. Galagovsky, L.R. Ramírez, J.A. |
| author_facet |
Tossi, V.E. Acebedo, S.L. Cassia, R.O. Lamattina, L. Galagovsky, L.R. Ramírez, J.A. |
| author_sort |
Tossi, V.E. |
| title |
A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| title_short |
A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| title_full |
A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| title_fullStr |
A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| title_full_unstemmed |
A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| title_sort |
bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production |
| url |
http://hdl.handle.net/20.500.12110/paper_0039128X_v102_n_p46_Tossi |
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1782027130184400896 |