A method to obtain monosporic cultures of arbuscular mycorrhizal fungi
We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermi...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia |
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todo:paper_00380717_v33_n9_p1283_Fracchia2023-10-03T14:48:19Z A method to obtain monosporic cultures of arbuscular mycorrhizal fungi Fracchia, S. Menendez, A. Godeas, A. Ocampo, J.A. Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible. © 2001 Elsevier Science Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium |
| spellingShingle |
Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium Fracchia, S. Menendez, A. Godeas, A. Ocampo, J.A. A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| topic_facet |
Arbuscular mycorrhizas Gigaspora Glomus Monosporic culture buffer concentration (parameters) culture vessel fungus spore mycorrhiza plant age plant fungus interaction seedling spore germination vermiculite arbuscular mycorrhiza fungus sampling Arbuscular mycorrhizae Bacteria (microorganisms) Fungi Gigaspora Gigaspora rosea Glomus Glomus mosseae Trifolium |
| description |
We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible. © 2001 Elsevier Science Ltd. All rights reserved. |
| format |
JOUR |
| author |
Fracchia, S. Menendez, A. Godeas, A. Ocampo, J.A. |
| author_facet |
Fracchia, S. Menendez, A. Godeas, A. Ocampo, J.A. |
| author_sort |
Fracchia, S. |
| title |
A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| title_short |
A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| title_full |
A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| title_fullStr |
A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| title_full_unstemmed |
A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| title_sort |
method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| url |
http://hdl.handle.net/20.500.12110/paper_00380717_v33_n9_p1283_Fracchia |
| work_keys_str_mv |
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1807320451552116736 |