Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds
Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately...
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todo:paper_0033183X_v231_n1-2_p1_Panza2023-10-03T14:45:31Z Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds Panza, V. Distéfano, A.J. Carjuzaa, P. Láinez, V. Del Vas, M. Maldonado, S. Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately 16, 18, and 24 kDa were identified in both samples analysed. Immunogold electron microscopy studies detected the presence of dehydrins in the embryo and endosperm. In both cases, dehydrins were immunolocalized in cytoplasm and chromatin. No labelling associated with either membranes or organelles was detected. It is known that dehydrins are produced as part of the developmental program of orthodox seeds and are also present in some recalcitrant seeds of temperate regions. The constitutive presence of dehydrins in embryos of extremely recalcitrant species of tropical origin has not been previously reported. © 2007 Springer-Verlag. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Láinez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0033183X_v231_n1-2_p1_Panza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis |
| spellingShingle |
Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis Panza, V. Distéfano, A.J. Carjuzaa, P. Láinez, V. Del Vas, M. Maldonado, S. Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| topic_facet |
Dehydrin Euterpe edulis Palmito Recalcitrant seed Subcellular immunolocalization Western blot dehydrin proteins, plant unclassified drug vegetable protein Arecaceae article metabolism plant plant seed ultrastructure Western blotting Arecaceae Blotting, Western Plant Proteins Plant Shoots Seeds Euterpe edulis |
| description |
Euterpe edulis Martius, a tropical palm species characterized as highly recalcitrant, accumulated dehydrin proteins in both the endosperm and the embryo of the mature seed, as detected by Western blot analysis and immunogold electron microscopy. Three major bands at molecular masses of approximately 16, 18, and 24 kDa were identified in both samples analysed. Immunogold electron microscopy studies detected the presence of dehydrins in the embryo and endosperm. In both cases, dehydrins were immunolocalized in cytoplasm and chromatin. No labelling associated with either membranes or organelles was detected. It is known that dehydrins are produced as part of the developmental program of orthodox seeds and are also present in some recalcitrant seeds of temperate regions. The constitutive presence of dehydrins in embryos of extremely recalcitrant species of tropical origin has not been previously reported. © 2007 Springer-Verlag. |
| format |
JOUR |
| author |
Panza, V. Distéfano, A.J. Carjuzaa, P. Láinez, V. Del Vas, M. Maldonado, S. |
| author_facet |
Panza, V. Distéfano, A.J. Carjuzaa, P. Láinez, V. Del Vas, M. Maldonado, S. |
| author_sort |
Panza, V. |
| title |
Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| title_short |
Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| title_full |
Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| title_fullStr |
Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| title_full_unstemmed |
Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds |
| title_sort |
detection of dehydrin-like proteins in embryos and endosperm of mature euterpe edulis seeds |
| url |
http://hdl.handle.net/20.500.12110/paper_0033183X_v231_n1-2_p1_Panza |
| work_keys_str_mv |
AT panzav detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT distefanoaj detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT carjuzaap detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT lainezv detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT delvasm detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds AT maldonados detectionofdehydrinlikeproteinsinembryosandendospermofmatureeuterpeedulisseeds |
| _version_ |
1782023488346783744 |