Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton
A comparative analysis of the effect of two compounds, dibutyryl-cyclic-AMP (dbcAMP) and latrunculin B, on the morphology and ultrastructure of the dimorphic fungus Mucor rouxii under aerobic growth conditions is presented. dbcAMP acts through the sustained activation of protein kinase A, and latrun...
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todo:paper_0033183X_v228_n4_p189_Pereyra2023-10-03T14:45:30Z Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton Pereyra, E. Ingerfeld, M. Anderson, N. Jackson, S.L. Moreno, S. Cell wall Cyclic-AMP signal transduction Cytoskeleton Latrunculin Mucor rouxii Scanning electron microscopy Transmission electron microscopy actin bucladesine cyclic AMP cyclic AMP dependent protein kinase drug derivative fused heterocyclic rings latrunculin B thiazolidine derivative article cytoskeleton drug effect fluorescence microscopy metabolism methodology Mucor scanning electron microscopy transmission electron microscopy ultrastructure Actins Bicyclo Compounds, Heterocyclic Bucladesine Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cytoskeleton Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Mucor Thiazolidines Amylomyces rouxii Fungi Mucor A comparative analysis of the effect of two compounds, dibutyryl-cyclic-AMP (dbcAMP) and latrunculin B, on the morphology and ultrastructure of the dimorphic fungus Mucor rouxii under aerobic growth conditions is presented. dbcAMP acts through the sustained activation of protein kinase A, and latrunculin B through the disruption of the actin cytoskeleton. Upon addition of these compounds to the growth medium at any stage of the germination process, cells lost polarised growth and switched to isodiametric growth. The effect was reversible. The morphologies, visualised by light microscopy or scanning electron microscopy (SEM), were alike. A switch from a rough to a smooth surface was observed by SEM when cells were repolarised by removal of the added compound. Ultrastructural changes under both conditions, as observed by transmission electron microscopy, were similar, the main feature being the enlargement of the cell wall, with irregular depositions, and detachment from the cell membrane. dbcAMP-treated cells showed a decrease in the number of glycogen granules compared with control and latrunculin B-treated cells. F-actin staining with fluorescein isothiocyanate-phalloidin showed that both dbcAMP- and latrunculin B-treated cells displayed a much lower fluorescence than control cells, with only a few pale plaques. The results suggest that the sustained activation of protein kinase A, which impairs polarised growth, might exert its effect through a modification of actin cytoskeleton organisation, very probably also involving an integrinlike pathway, as judged by the cell wall detachment and loss of cell adhesiveness of the dbcAMP-treated isodiametric cells. © Springer-Verlag 2006. Fil:Pereyra, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0033183X_v228_n4_p189_Pereyra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell wall Cyclic-AMP signal transduction Cytoskeleton Latrunculin Mucor rouxii Scanning electron microscopy Transmission electron microscopy actin bucladesine cyclic AMP cyclic AMP dependent protein kinase drug derivative fused heterocyclic rings latrunculin B thiazolidine derivative article cytoskeleton drug effect fluorescence microscopy metabolism methodology Mucor scanning electron microscopy transmission electron microscopy ultrastructure Actins Bicyclo Compounds, Heterocyclic Bucladesine Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cytoskeleton Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Mucor Thiazolidines Amylomyces rouxii Fungi Mucor |
| spellingShingle |
Cell wall Cyclic-AMP signal transduction Cytoskeleton Latrunculin Mucor rouxii Scanning electron microscopy Transmission electron microscopy actin bucladesine cyclic AMP cyclic AMP dependent protein kinase drug derivative fused heterocyclic rings latrunculin B thiazolidine derivative article cytoskeleton drug effect fluorescence microscopy metabolism methodology Mucor scanning electron microscopy transmission electron microscopy ultrastructure Actins Bicyclo Compounds, Heterocyclic Bucladesine Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cytoskeleton Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Mucor Thiazolidines Amylomyces rouxii Fungi Mucor Pereyra, E. Ingerfeld, M. Anderson, N. Jackson, S.L. Moreno, S. Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| topic_facet |
Cell wall Cyclic-AMP signal transduction Cytoskeleton Latrunculin Mucor rouxii Scanning electron microscopy Transmission electron microscopy actin bucladesine cyclic AMP cyclic AMP dependent protein kinase drug derivative fused heterocyclic rings latrunculin B thiazolidine derivative article cytoskeleton drug effect fluorescence microscopy metabolism methodology Mucor scanning electron microscopy transmission electron microscopy ultrastructure Actins Bicyclo Compounds, Heterocyclic Bucladesine Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cytoskeleton Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Mucor Thiazolidines Amylomyces rouxii Fungi Mucor |
| description |
A comparative analysis of the effect of two compounds, dibutyryl-cyclic-AMP (dbcAMP) and latrunculin B, on the morphology and ultrastructure of the dimorphic fungus Mucor rouxii under aerobic growth conditions is presented. dbcAMP acts through the sustained activation of protein kinase A, and latrunculin B through the disruption of the actin cytoskeleton. Upon addition of these compounds to the growth medium at any stage of the germination process, cells lost polarised growth and switched to isodiametric growth. The effect was reversible. The morphologies, visualised by light microscopy or scanning electron microscopy (SEM), were alike. A switch from a rough to a smooth surface was observed by SEM when cells were repolarised by removal of the added compound. Ultrastructural changes under both conditions, as observed by transmission electron microscopy, were similar, the main feature being the enlargement of the cell wall, with irregular depositions, and detachment from the cell membrane. dbcAMP-treated cells showed a decrease in the number of glycogen granules compared with control and latrunculin B-treated cells. F-actin staining with fluorescein isothiocyanate-phalloidin showed that both dbcAMP- and latrunculin B-treated cells displayed a much lower fluorescence than control cells, with only a few pale plaques. The results suggest that the sustained activation of protein kinase A, which impairs polarised growth, might exert its effect through a modification of actin cytoskeleton organisation, very probably also involving an integrinlike pathway, as judged by the cell wall detachment and loss of cell adhesiveness of the dbcAMP-treated isodiametric cells. © Springer-Verlag 2006. |
| format |
JOUR |
| author |
Pereyra, E. Ingerfeld, M. Anderson, N. Jackson, S.L. Moreno, S. |
| author_facet |
Pereyra, E. Ingerfeld, M. Anderson, N. Jackson, S.L. Moreno, S. |
| author_sort |
Pereyra, E. |
| title |
Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| title_short |
Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| title_full |
Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| title_fullStr |
Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| title_full_unstemmed |
Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton |
| title_sort |
mucor rouxii ultrastructure: cyclic amp and actin cytoskeleton |
| url |
http://hdl.handle.net/20.500.12110/paper_0033183X_v228_n4_p189_Pereyra |
| work_keys_str_mv |
AT pereyrae mucorrouxiiultrastructurecyclicampandactincytoskeleton AT ingerfeldm mucorrouxiiultrastructurecyclicampandactincytoskeleton AT andersonn mucorrouxiiultrastructurecyclicampandactincytoskeleton AT jacksonsl mucorrouxiiultrastructurecyclicampandactincytoskeleton AT morenos mucorrouxiiultrastructurecyclicampandactincytoskeleton |
| _version_ |
1807322393784352768 |