RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii
The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with...
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todo:paper_0033183X_v222_n1-2_p23_Pereyra2023-10-03T14:45:30Z RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii Pereyra, E. Argimón, S. Jackson, S.L. Moreno, S. Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with 3.6 times more germ tubes per cell than control germinating sporangiospores. Disruption of the actin network with 2 μg of cytochalasin A per ml blocked the switch to tip growth; the effect was analogous to the one of 150 μM dibutyryl-cyclic AMP (cAMP), which we previously described to promote isodiametric growth via protein kinase A. 150 μM dibutyryl-cAMP antagonises partially the effect of 1 mM RGD; the cells still emit several germ tubes per mother cell but their number is smaller and the volume of the cell at germ tube emission is larger than with RGD alone. At higher concentrations the dibutyryl-cAMP overrides completely the effect of RGD. Our results suggest that M. rouxii has an RGD recognition system and demonstrate that RGD-containing peptides have a profound effect on the isotropic stage of growth and on the establishment of cell polarity and that cAMP analogues can override this effect. Fil:Pereyra, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Argimón, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0033183X_v222_n1-2_p23_Pereyra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor |
| spellingShingle |
Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor Pereyra, E. Argimón, S. Jackson, S.L. Moreno, S. RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| topic_facet |
Arg-Gly-Asp tripeptide Cell morphology Cyclic AMP Mucor rouxii ProNectin F Actins Cell Size Cyclic AMP Cytochalasins Fibronectins Mucor Oligopeptides Recombinant Proteins Signal Transduction Spores, Fungal Amylomyces rouxii Fungi Mucor |
| description |
The tripeptide Arg-Gly-Asp (RGD) (1 mM) as well as the polymer ProNectin F (20 nM) added to culture medium of the fungus Mucor rouxii (defined medium) produced a delay in the switch from isodiametric growth to tip growth; at the time of germination the mother cell had a 4.6 times larger volume with 3.6 times more germ tubes per cell than control germinating sporangiospores. Disruption of the actin network with 2 μg of cytochalasin A per ml blocked the switch to tip growth; the effect was analogous to the one of 150 μM dibutyryl-cyclic AMP (cAMP), which we previously described to promote isodiametric growth via protein kinase A. 150 μM dibutyryl-cAMP antagonises partially the effect of 1 mM RGD; the cells still emit several germ tubes per mother cell but their number is smaller and the volume of the cell at germ tube emission is larger than with RGD alone. At higher concentrations the dibutyryl-cAMP overrides completely the effect of RGD. Our results suggest that M. rouxii has an RGD recognition system and demonstrate that RGD-containing peptides have a profound effect on the isotropic stage of growth and on the establishment of cell polarity and that cAMP analogues can override this effect. |
| format |
JOUR |
| author |
Pereyra, E. Argimón, S. Jackson, S.L. Moreno, S. |
| author_facet |
Pereyra, E. Argimón, S. Jackson, S.L. Moreno, S. |
| author_sort |
Pereyra, E. |
| title |
RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| title_short |
RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| title_full |
RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| title_fullStr |
RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| title_full_unstemmed |
RGD-containing peptides and cyclic AMP have antagonistic roles in the morphology of Mucor rouxii |
| title_sort |
rgd-containing peptides and cyclic amp have antagonistic roles in the morphology of mucor rouxii |
| url |
http://hdl.handle.net/20.500.12110/paper_0033183X_v222_n1-2_p23_Pereyra |
| work_keys_str_mv |
AT pereyrae rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii AT argimons rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii AT jacksonsl rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii AT morenos rgdcontainingpeptidesandcyclicamphaveantagonisticrolesinthemorphologyofmucorrouxii |
| _version_ |
1807323165648486400 |