Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation

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Potato (Solanum tuberosum L.) tuber UDP-glucose:protein transglucosylase (UPTG) (EC 2.4.1.112) is involved in the first of a two-step mechanism proposed for protein-bound α-glucan synthesis by catalyzing the covalent attachment of a single glucose residue to an acceptor protein. The resulting glucos...

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Autores principales: Ardila, F.J., Tandecarz, J.S.
Formato: JOUR
Materias:
Solanum tuberosum
Tuberosum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00320889_v99_n4_p1342_Ardila
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00320889_v99_n4_p1342_Ardila
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spelling todo:paper_00320889_v99_n4_p1342_Ardila2023-10-03T14:45:00Z Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation Ardila, F.J. Tandecarz, J.S. Solanum tuberosum Tuberosum Potato (Solanum tuberosum L.) tuber UDP-glucose:protein transglucosylase (UPTG) (EC 2.4.1.112) is involved in the first of a two-step mechanism proposed for protein-bound α-glucan synthesis by catalyzing the covalent attachment of a single glucose residue to an acceptor protein. The resulting glucosylated 38-kilodalton polypeptide would then serve as a primer for enzymic glucan chain elongation during the second step. In the present report, we describe the fast protein liquid chromatography purification of UPTG from a membrane pellet of potato tuber. An apparently close association of UPTG, phosphorylase, and starch synthase was observed under native conditions during different purification steps. Enrichment of a 38-kilodalton polypeptide was found throughout enzyme purification. It is now shown that the purified UPTG, with an apparent molecular mass of 38 kilodaltons, undergoes self-glucosylation in a UDP-glucose- and Mn2+-dependent reaction. Therefore, it is concluded that UPTG is the enzyme and at the same time the priming protein required for the biogenesis of protein-bound α-glucan in potato tuber. Fil:Ardila, F.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320889_v99_n4_p1342_Ardila
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Solanum tuberosum
Tuberosum
spellingShingle Solanum tuberosum
Tuberosum
Ardila, F.J.
Tandecarz, J.S.
Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
topic_facet Solanum tuberosum
Tuberosum
description Potato (Solanum tuberosum L.) tuber UDP-glucose:protein transglucosylase (UPTG) (EC 2.4.1.112) is involved in the first of a two-step mechanism proposed for protein-bound α-glucan synthesis by catalyzing the covalent attachment of a single glucose residue to an acceptor protein. The resulting glucosylated 38-kilodalton polypeptide would then serve as a primer for enzymic glucan chain elongation during the second step. In the present report, we describe the fast protein liquid chromatography purification of UPTG from a membrane pellet of potato tuber. An apparently close association of UPTG, phosphorylase, and starch synthase was observed under native conditions during different purification steps. Enrichment of a 38-kilodalton polypeptide was found throughout enzyme purification. It is now shown that the purified UPTG, with an apparent molecular mass of 38 kilodaltons, undergoes self-glucosylation in a UDP-glucose- and Mn2+-dependent reaction. Therefore, it is concluded that UPTG is the enzyme and at the same time the priming protein required for the biogenesis of protein-bound α-glucan in potato tuber.
format JOUR
author Ardila, F.J.
Tandecarz, J.S.
author_facet Ardila, F.J.
Tandecarz, J.S.
author_sort Ardila, F.J.
title Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
title_short Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
title_full Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
title_fullStr Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
title_full_unstemmed Potato tuber UDP-Glucose: Protein transglucosylase catalyzes its own glucosylation
title_sort potato tuber udp-glucose: protein transglucosylase catalyzes its own glucosylation
url http://hdl.handle.net/20.500.12110/paper_00320889_v99_n4_p1342_Ardila
work_keys_str_mv AT ardilafj potatotuberudpglucoseproteintransglucosylasecatalyzesitsownglucosylation
AT tandecarzjs potatotuberudpglucoseproteintransglucosylasecatalyzesitsownglucosylation
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