Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions

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To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hy...

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Autores principales: Sutka, M., Li, G., Boudet, J., Boursiac, Y., Doumas, P., Maurel, C.
Formato: JOUR
Materias:
aquaporin
messenger RNA
sodium chloride
water
Arabidopsis
article
drug effect
gene expression profiling
gene expression regulation
genetic variability
genetics
growth, development and aging
histology
metabolism
physiological stress
physiology
plant root
principal component analysis
Aquaporins
Gene Expression Profiling
Gene Expression Regulation, Plant
Genetic Variation
Plant Roots
Principal Component Analysis
RNA, Messenger
Sodium Chloride
Stress, Physiological
Water
Arabidopsis thaliana
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00320889_v155_n3_p1264_Sutka
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00320889_v155_n3_p1264_Sutka
record_format dspace
spelling todo:paper_00320889_v155_n3_p1264_Sutka2023-10-03T14:44:57Z Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions Sutka, M. Li, G. Boudet, J. Boursiac, Y. Doumas, P. Maurel, C. aquaporin messenger RNA sodium chloride water Arabidopsis article drug effect gene expression profiling gene expression regulation genetic variability genetics growth, development and aging histology metabolism physiological stress physiology plant root principal component analysis Aquaporins Arabidopsis Gene Expression Profiling Gene Expression Regulation, Plant Genetic Variation Plant Roots Principal Component Analysis RNA, Messenger Sodium Chloride Stress, Physiological Water Arabidopsis Arabidopsis thaliana To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics. © 2011 American Society of Plant Biologists. Fil:Sutka, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320889_v155_n3_p1264_Sutka
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic aquaporin
messenger RNA
sodium chloride
water
Arabidopsis
article
drug effect
gene expression profiling
gene expression regulation
genetic variability
genetics
growth, development and aging
histology
metabolism
physiological stress
physiology
plant root
principal component analysis
Aquaporins
Arabidopsis
Gene Expression Profiling
Gene Expression Regulation, Plant
Genetic Variation
Plant Roots
Principal Component Analysis
RNA, Messenger
Sodium Chloride
Stress, Physiological
Water
Arabidopsis
Arabidopsis thaliana
spellingShingle aquaporin
messenger RNA
sodium chloride
water
Arabidopsis
article
drug effect
gene expression profiling
gene expression regulation
genetic variability
genetics
growth, development and aging
histology
metabolism
physiological stress
physiology
plant root
principal component analysis
Aquaporins
Arabidopsis
Gene Expression Profiling
Gene Expression Regulation, Plant
Genetic Variation
Plant Roots
Principal Component Analysis
RNA, Messenger
Sodium Chloride
Stress, Physiological
Water
Arabidopsis
Arabidopsis thaliana
Sutka, M.
Li, G.
Boudet, J.
Boursiac, Y.
Doumas, P.
Maurel, C.
Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
topic_facet aquaporin
messenger RNA
sodium chloride
water
Arabidopsis
article
drug effect
gene expression profiling
gene expression regulation
genetic variability
genetics
growth, development and aging
histology
metabolism
physiological stress
physiology
plant root
principal component analysis
Aquaporins
Arabidopsis
Gene Expression Profiling
Gene Expression Regulation, Plant
Genetic Variation
Plant Roots
Principal Component Analysis
RNA, Messenger
Sodium Chloride
Stress, Physiological
Water
Arabidopsis
Arabidopsis thaliana
description To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to root water transport and plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 natural accessions of Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity of excised root systems (Lpr) showed a 2-fold variation among accessions. The contribution of aquaporins to water uptake was characterized using as inhibitors mercury, propionic acid, and azide. The aquaporin-dependent and -independent paths of water transport made variable contributions to the total hydraulic conductivity in the different accessions. The distinct suberization patterns observed among accessions were not correlated with their root hydraulic properties. Real-time reverse transcription-polymerase chain reaction revealed, by contrast, a positive overall correlation between Lpr and certain highly expressed PIP transcripts. Root hydraulic responses to salt stress were characterized in a subset of five accessions (Bulhary-1, Catania-1, Columbia-0, Dijon-M, and Monte-Tosso-0 [Mr-0]). Lpr was down-regulated in all accessions except Mr-0. In Mr-0 and Catania-1, cortical cell hydraulic conductivity was unresponsive to salt, whereas it was down-regulated in the three other accessions. By contrast, the five accessions showed qualitatively similar aquaporin transcriptional profiles in response to salt. The overall work provides clues on how hydraulic regulation allows plant adaptation to salt stress. It also shows that a wide range of root hydraulic profiles, as previously reported in various species, can be observed in a single model species. This work paves the way for a quantitative genetics analysis of root hydraulics. © 2011 American Society of Plant Biologists.
format JOUR
author Sutka, M.
Li, G.
Boudet, J.
Boursiac, Y.
Doumas, P.
Maurel, C.
author_facet Sutka, M.
Li, G.
Boudet, J.
Boursiac, Y.
Doumas, P.
Maurel, C.
author_sort Sutka, M.
title Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_short Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_full Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_fullStr Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_full_unstemmed Natural variation of root hydraulics in Arabidopsis grown in normal and salt-stressed conditions
title_sort natural variation of root hydraulics in arabidopsis grown in normal and salt-stressed conditions
url http://hdl.handle.net/20.500.12110/paper_00320889_v155_n3_p1264_Sutka
work_keys_str_mv AT sutkam naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
AT lig naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
AT boudetj naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
AT boursiacy naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
AT doumasp naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
AT maurelc naturalvariationofroothydraulicsinarabidopsisgrowninnormalandsaltstressedconditions
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