Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination

In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from...

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Autores principales: Roqueiro, G., Causin, F., Olle-Resa, C., Maroder, H., Maldonado, S.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro
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spelling todo:paper_00319317_v149_n2_p286_Roqueiro2023-10-03T14:43:38Z Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination Roqueiro, G. Causin, F. Olle-Resa, C. Maroder, H. Maldonado, S. Salix antioxidant ascorbate peroxidase catalase guaiacol peroxidase malonaldehyde peroxidase reactive oxygen metabolite superoxide dismutase analysis of variance article chloroplast cotyledon germination growth, development and aging light lipid peroxidation metabolism oxidation reduction reaction physiological stress plant leaf plant root plant seed radiation exposure seedling time transmission electron microscopy ultrastructure willow Analysis of Variance Antioxidants Ascorbate Peroxidases Catalase Chloroplasts Cotyledon Germination Light Lipid Peroxidation Malondialdehyde Microscopy, Electron, Transmission Oxidation-Reduction Peroxidase Plant Leaves Plant Roots Reactive Oxygen Species Salix Seedling Seeds Stress, Physiological Superoxide Dismutase Time Factors In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. © 2013 Scandinavian Plant Physiology Society. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Causin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Salix
antioxidant
ascorbate peroxidase
catalase
guaiacol peroxidase
malonaldehyde
peroxidase
reactive oxygen metabolite
superoxide dismutase
analysis of variance
article
chloroplast
cotyledon
germination
growth, development and aging
light
lipid peroxidation
metabolism
oxidation reduction reaction
physiological stress
plant leaf
plant root
plant seed
radiation exposure
seedling
time
transmission electron microscopy
ultrastructure
willow
Analysis of Variance
Antioxidants
Ascorbate Peroxidases
Catalase
Chloroplasts
Cotyledon
Germination
Light
Lipid Peroxidation
Malondialdehyde
Microscopy, Electron, Transmission
Oxidation-Reduction
Peroxidase
Plant Leaves
Plant Roots
Reactive Oxygen Species
Salix
Seedling
Seeds
Stress, Physiological
Superoxide Dismutase
Time Factors
spellingShingle Salix
antioxidant
ascorbate peroxidase
catalase
guaiacol peroxidase
malonaldehyde
peroxidase
reactive oxygen metabolite
superoxide dismutase
analysis of variance
article
chloroplast
cotyledon
germination
growth, development and aging
light
lipid peroxidation
metabolism
oxidation reduction reaction
physiological stress
plant leaf
plant root
plant seed
radiation exposure
seedling
time
transmission electron microscopy
ultrastructure
willow
Analysis of Variance
Antioxidants
Ascorbate Peroxidases
Catalase
Chloroplasts
Cotyledon
Germination
Light
Lipid Peroxidation
Malondialdehyde
Microscopy, Electron, Transmission
Oxidation-Reduction
Peroxidase
Plant Leaves
Plant Roots
Reactive Oxygen Species
Salix
Seedling
Seeds
Stress, Physiological
Superoxide Dismutase
Time Factors
Roqueiro, G.
Causin, F.
Olle-Resa, C.
Maroder, H.
Maldonado, S.
Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
topic_facet Salix
antioxidant
ascorbate peroxidase
catalase
guaiacol peroxidase
malonaldehyde
peroxidase
reactive oxygen metabolite
superoxide dismutase
analysis of variance
article
chloroplast
cotyledon
germination
growth, development and aging
light
lipid peroxidation
metabolism
oxidation reduction reaction
physiological stress
plant leaf
plant root
plant seed
radiation exposure
seedling
time
transmission electron microscopy
ultrastructure
willow
Analysis of Variance
Antioxidants
Ascorbate Peroxidases
Catalase
Chloroplasts
Cotyledon
Germination
Light
Lipid Peroxidation
Malondialdehyde
Microscopy, Electron, Transmission
Oxidation-Reduction
Peroxidase
Plant Leaves
Plant Roots
Reactive Oxygen Species
Salix
Seedling
Seeds
Stress, Physiological
Superoxide Dismutase
Time Factors
description In willow seeds, photooxidative damage is mainly restricted to the outer cotyledonary tissues, significantly reducing normal germination. Here we analyzed the damage generated in cotyledonary tissues and investigated whether the increase in reactive oxygen species (ROS) generation in seedlings from photooxidized seeds can affect the morphogenetic capacity of the shoot apical meristem. Seeds were photooxidized under different light intensities and the evolution of the damage during seedling growth was studied by light and transmission electron microscopies. The level of lipid peroxidation and changes in antioxidant capacity were measured following the time course of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase enzyme activities, and the effect of photooxidative stress on the genesis of new leaf primordia and lateral roots was examined. Early and active endocytosis and autophagy, changes in chloroplast morphology, as well as the accumulation and diffusion of ROS all play important roles in the early cell death observed in cotyledonary tissues. Following germination, seedlings from photooxidized seeds anticipated the emergence of first leaves, which complemented the altered functionality of the damaged cotyledons. © 2013 Scandinavian Plant Physiology Society.
format JOUR
author Roqueiro, G.
Causin, F.
Olle-Resa, C.
Maroder, H.
Maldonado, S.
author_facet Roqueiro, G.
Causin, F.
Olle-Resa, C.
Maroder, H.
Maldonado, S.
author_sort Roqueiro, G.
title Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
title_short Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
title_full Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
title_fullStr Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
title_full_unstemmed Willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: A histological and biochemical study following germination
title_sort willow seedlings from photooxidized seeds accelerate cotyledon death and anticipate first leaf emergence: a histological and biochemical study following germination
url http://hdl.handle.net/20.500.12110/paper_00319317_v149_n2_p286_Roqueiro
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AT causinf willowseedlingsfromphotooxidizedseedsacceleratecotyledondeathandanticipatefirstleafemergenceahistologicalandbiochemicalstudyfollowinggermination
AT olleresac willowseedlingsfromphotooxidizedseedsacceleratecotyledondeathandanticipatefirstleafemergenceahistologicalandbiochemicalstudyfollowinggermination
AT maroderh willowseedlingsfromphotooxidizedseedsacceleratecotyledondeathandanticipatefirstleafemergenceahistologicalandbiochemicalstudyfollowinggermination
AT maldonados willowseedlingsfromphotooxidizedseedsacceleratecotyledondeathandanticipatefirstleafemergenceahistologicalandbiochemicalstudyfollowinggermination
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