ASR1, a stress-induced tomato protein, protects yeast from osmotic stress

Mostrar todas las versiones(3)

Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expressi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Moretti, M.B., Maskin, L., Gudesblat, G., García, S.C., Iusem, N.D.
Formato: JOUR
Materias:
Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00319317_v127_n1_p111_Moretti
record_format dspace
spelling todo:paper_00319317_v127_n1_p111_Moretti2023-10-03T14:43:38Z ASR1, a stress-induced tomato protein, protects yeast from osmotic stress Moretti, M.B. Maskin, L. Gudesblat, G. García, S.C. Iusem, N.D. Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
spellingShingle Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
topic_facet Biosynthesis
Fruits
Genes
Glycerol
Osmosis
Yeast
Endogenous glycerol
Osmotic stress
Tomato protein
Proteins
Tomatoes
Lycopersicon esculentum
Saccharomyces cerevisiae
description Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006.
format JOUR
author Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
author_facet Moretti, M.B.
Maskin, L.
Gudesblat, G.
García, S.C.
Iusem, N.D.
author_sort Moretti, M.B.
title ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_short ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_fullStr ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full_unstemmed ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_sort asr1, a stress-induced tomato protein, protects yeast from osmotic stress
url http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
work_keys_str_mv AT morettimb asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
AT maskinl asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
AT gudesblatg asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
AT garciasc asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
AT iusemnd asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
_version_ 1782023920873897984

Ejemplares similares