Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions

The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl2) in two Chlamydomonas reinhardtii strains: The wild type 4A+ and the pleiotropic mutant cur9, originally selected as a coppertolerant phenotype in mixotrophic conditions. Bioassays were performed in...

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Autores principales: Sabatini, S.E., Leonardi, P.I., Rodríguez, M.C.
Formato: JOUR
Materias:
Autotrophic and mixotrophic growth
Catalase
Chlamydomonas reinhardtii
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlorophyceae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00318884_v50_n1_p78_Sabatini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00318884_v50_n1_p78_Sabatini
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spelling todo:paper_00318884_v50_n1_p78_Sabatini2023-10-03T14:41:46Z Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions Sabatini, S.E. Leonardi, P.I. Rodríguez, M.C. Autotrophic and mixotrophic growth Catalase Chlamydomonas reinhardtii Isocitrate lyase Lipid peroxidation Oxidative stress Chlamydomonadales Chlamydomonas reinhardtii Chlorophyceae The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl2) in two Chlamydomonas reinhardtii strains: The wild type 4A+ and the pleiotropic mutant cur9, originally selected as a coppertolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mmol m22 s21). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobarbituric acid reactive substances (TBARS) and growth rate, demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72 h incubation. An inverse relation between catalase (CAT) and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as depression in growth rate or increase in TBARS dosage. Fil:Sabatini, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00318884_v50_n1_p78_Sabatini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Autotrophic and mixotrophic growth
Catalase
Chlamydomonas reinhardtii
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
spellingShingle Autotrophic and mixotrophic growth
Catalase
Chlamydomonas reinhardtii
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
Sabatini, S.E.
Leonardi, P.I.
Rodríguez, M.C.
Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
topic_facet Autotrophic and mixotrophic growth
Catalase
Chlamydomonas reinhardtii
Isocitrate lyase
Lipid peroxidation
Oxidative stress
Chlamydomonadales
Chlamydomonas reinhardtii
Chlorophyceae
description The aim of this work was to determine the responses to sublethal copper exposure (20 and 40 mM CuCl2) in two Chlamydomonas reinhardtii strains: The wild type 4A+ and the pleiotropic mutant cur9, originally selected as a coppertolerant phenotype in mixotrophic conditions. Bioassays were performed in both autotrophic and mixotrophic media, under low irradiation (50 mmol m22 s21). At the onset, Cu2+ concentration in culture media was estimated by MINEQL software. Oxidative stress indicators, such as changes in photosynthetic pigment ratios, catalase activity, concentration of thiobarbituric acid reactive substances (TBARS) and growth rate, demonstrated a better performance of the wild type and the mutant strain in autotrophic and mixotrophic conditions, respectively. Moderate oxidative stress was accompanied by ultrastructural changes, such as accumulation of starch and vacuoles with electronic-dense deposits and membranous structures together with mild thylakoidal and mitochondrial crest disorganization. In mixotrophic conditions isocitrate lyase (ICL) activity peaked after 72 h incubation. An inverse relation between catalase (CAT) and ICL activities was verified and proved to be a good indicator of oxidative stress correlating consistently with other characteristic features such as depression in growth rate or increase in TBARS dosage.
format JOUR
author Sabatini, S.E.
Leonardi, P.I.
Rodríguez, M.C.
author_facet Sabatini, S.E.
Leonardi, P.I.
Rodríguez, M.C.
author_sort Sabatini, S.E.
title Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_short Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_full Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_fullStr Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_full_unstemmed Responses to sublethal copper exposure in two strains of Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) in autotrophic and mixotrophic conditions
title_sort responses to sublethal copper exposure in two strains of chlamydomonas reinhardtii (volvocales, chlorophyceae) in autotrophic and mixotrophic conditions
url http://hdl.handle.net/20.500.12110/paper_00318884_v50_n1_p78_Sabatini
work_keys_str_mv AT sabatinise responsestosublethalcopperexposureintwostrainsofchlamydomonasreinhardtiivolvocaleschlorophyceaeinautotrophicandmixotrophicconditions
AT leonardipi responsestosublethalcopperexposureintwostrainsofchlamydomonasreinhardtiivolvocaleschlorophyceaeinautotrophicandmixotrophicconditions
AT rodriguezmc responsestosublethalcopperexposureintwostrainsofchlamydomonasreinhardtiivolvocaleschlorophyceaeinautotrophicandmixotrophicconditions
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