Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)

The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fuco...

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Autores principales: Ponce, N.M.A., Leonardi, P.I., Flores, M.L., Stortz, C.A., Rodríguez, M.C.
Formato: JOUR
Materias:
Adenocystis utricularis
Alginate
Cell wall polysaccharides
Fucoidan
Histochemistry
algae
Ectocarpales
Phaeophyceae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00318884_v46_n6_p675_Ponce
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00318884_v46_n6_p675_Ponce
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spelling todo:paper_00318884_v46_n6_p675_Ponce2023-10-03T14:41:45Z Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae) Ponce, N.M.A. Leonardi, P.I. Flores, M.L. Stortz, C.A. Rodríguez, M.C. Adenocystis utricularis Alginate Cell wall polysaccharides Fucoidan Histochemistry Adenocystis utricularis algae Ectocarpales Phaeophyceae The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. "Egg-box" structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00318884_v46_n6_p675_Ponce
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Adenocystis utricularis
Alginate
Cell wall polysaccharides
Fucoidan
Histochemistry
Adenocystis utricularis
algae
Ectocarpales
Phaeophyceae
spellingShingle Adenocystis utricularis
Alginate
Cell wall polysaccharides
Fucoidan
Histochemistry
Adenocystis utricularis
algae
Ectocarpales
Phaeophyceae
Ponce, N.M.A.
Leonardi, P.I.
Flores, M.L.
Stortz, C.A.
Rodríguez, M.C.
Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
topic_facet Adenocystis utricularis
Alginate
Cell wall polysaccharides
Fucoidan
Histochemistry
Adenocystis utricularis
algae
Ectocarpales
Phaeophyceae
description The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. "Egg-box" structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides.
format JOUR
author Ponce, N.M.A.
Leonardi, P.I.
Flores, M.L.
Stortz, C.A.
Rodríguez, M.C.
author_facet Ponce, N.M.A.
Leonardi, P.I.
Flores, M.L.
Stortz, C.A.
Rodríguez, M.C.
author_sort Ponce, N.M.A.
title Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_short Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_full Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_fullStr Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_full_unstemmed Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_sort polysaccharide localization in the sporophyte cell wall of adenocystis utricularis (ectocarpales s.l., phaeophyceae)
url http://hdl.handle.net/20.500.12110/paper_00318884_v46_n6_p675_Ponce
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AT leonardipi polysaccharidelocalizationinthesporophytecellwallofadenocystisutricularisectocarpalesslphaeophyceae
AT floresml polysaccharidelocalizationinthesporophytecellwallofadenocystisutricularisectocarpalesslphaeophyceae
AT stortzca polysaccharidelocalizationinthesporophytecellwallofadenocystisutricularisectocarpalesslphaeophyceae
AT rodriguezmc polysaccharidelocalizationinthesporophytecellwallofadenocystisutricularisectocarpalesslphaeophyceae
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