Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids

The present study explores whether some arachidonic acid (AA) metabolites formed via cyclo-oxygenase or lipoxygenase pathways influence the inotropism of isolated rat urinary bladders evoked by electric field stimulation (EFS), both in presence of or in absence of atropine. The EFS consisted in squa...

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Autores principales: Dveksler, G., Gimeno, M.F., Gimeno, A.L.
Formato: JOUR
Materias:
priority journal
Animal
Aspirin
Atropine
Bladder
Eicosanoic Acids
Electric Stimulation
Female
In Vitro
Muscle Contraction
Muscle, Smooth
Parasympathetic Nervous System
Prostaglandins
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00316989_v19_n4_p295_Dveksler
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00316989_v19_n4_p295_Dveksler
record_format dspace
spelling todo:paper_00316989_v19_n4_p295_Dveksler2023-10-03T14:41:29Z Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids Dveksler, G. Gimeno, M.F. Gimeno, A.L. priority journal Animal Aspirin Atropine Bladder Eicosanoic Acids Electric Stimulation Female In Vitro Muscle Contraction Muscle, Smooth Parasympathetic Nervous System Prostaglandins Rats Rats, Inbred Strains Support, Non-U.S. Gov't The present study explores whether some arachidonic acid (AA) metabolites formed via cyclo-oxygenase or lipoxygenase pathways influence the inotropism of isolated rat urinary bladders evoked by electric field stimulation (EFS), both in presence of or in absence of atropine. The EFS consisted in square wave pulses of 5 Hz, 5 msec duration and supramaximal voltage, applied every 3 min for a period of 10 sec. Acetylsalicylic acid (ASA) at 10-4M, decreased around 30% the magnitude of inotropic responses to EFS and prostaglandin E2 (PGE2, partially at 10-9M and fully at 10-8M), restored control inotropic levels. PGI2 was also active but only at 10-7M); whereas PGF2α, at all concentrations tested (10-9-10-7M), failed to counter act the diminishing effect of ASA. Moreover, dose-response curves for added acetylcholine (ACh) were not modified by ASA. On the other hand, in presence of atropine (10-5M), a diminution of around 40% in the magnitude of control responses to EFS, was detected and when ASA (10-4M) was added to atropine-exposed preparations, the atropine resistant component of the EFS-evoked inotropism, was reduced around 30%. Although recovering effects of added PGs were again observed, they were not identical to those observed in absence of atropine. Indeed, PGE2 fully restored the magnitude of contractile responses but it did so only at concentrations 10 times higher than without the cholinergic receptor blocker. On the other hand, PGI2 as well as PGF2 α, even at 10-7M, were devoid of significant influence. In determining whether lipoxygenase products of AA were also able to modulate the EFS-evoked inotropism, tissue preparations were incubated with nordihydroguaiaretic acid (NDGA), an inhibitor of lipoxygenases, and tested for contractile responses to electric stimulation. NDGA (10-7M), failed to alter rat urinary bladder inotropic response to EFS, both in presence of or in absence of atropine. Results suggest that the cholinergic, as well as the non-cholinergic components of the EFS-evoked contractions in isolated rat urinary bladders, are modulated by some cyclo-oxygenase formed AA metabolites, particularly certain PGs, like PGE2. © 1987. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00316989_v19_n4_p295_Dveksler
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic priority journal
Animal
Aspirin
Atropine
Bladder
Eicosanoic Acids
Electric Stimulation
Female
In Vitro
Muscle Contraction
Muscle, Smooth
Parasympathetic Nervous System
Prostaglandins
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
spellingShingle priority journal
Animal
Aspirin
Atropine
Bladder
Eicosanoic Acids
Electric Stimulation
Female
In Vitro
Muscle Contraction
Muscle, Smooth
Parasympathetic Nervous System
Prostaglandins
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Dveksler, G.
Gimeno, M.F.
Gimeno, A.L.
Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
topic_facet priority journal
Animal
Aspirin
Atropine
Bladder
Eicosanoic Acids
Electric Stimulation
Female
In Vitro
Muscle Contraction
Muscle, Smooth
Parasympathetic Nervous System
Prostaglandins
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
description The present study explores whether some arachidonic acid (AA) metabolites formed via cyclo-oxygenase or lipoxygenase pathways influence the inotropism of isolated rat urinary bladders evoked by electric field stimulation (EFS), both in presence of or in absence of atropine. The EFS consisted in square wave pulses of 5 Hz, 5 msec duration and supramaximal voltage, applied every 3 min for a period of 10 sec. Acetylsalicylic acid (ASA) at 10-4M, decreased around 30% the magnitude of inotropic responses to EFS and prostaglandin E2 (PGE2, partially at 10-9M and fully at 10-8M), restored control inotropic levels. PGI2 was also active but only at 10-7M); whereas PGF2α, at all concentrations tested (10-9-10-7M), failed to counter act the diminishing effect of ASA. Moreover, dose-response curves for added acetylcholine (ACh) were not modified by ASA. On the other hand, in presence of atropine (10-5M), a diminution of around 40% in the magnitude of control responses to EFS, was detected and when ASA (10-4M) was added to atropine-exposed preparations, the atropine resistant component of the EFS-evoked inotropism, was reduced around 30%. Although recovering effects of added PGs were again observed, they were not identical to those observed in absence of atropine. Indeed, PGE2 fully restored the magnitude of contractile responses but it did so only at concentrations 10 times higher than without the cholinergic receptor blocker. On the other hand, PGI2 as well as PGF2 α, even at 10-7M, were devoid of significant influence. In determining whether lipoxygenase products of AA were also able to modulate the EFS-evoked inotropism, tissue preparations were incubated with nordihydroguaiaretic acid (NDGA), an inhibitor of lipoxygenases, and tested for contractile responses to electric stimulation. NDGA (10-7M), failed to alter rat urinary bladder inotropic response to EFS, both in presence of or in absence of atropine. Results suggest that the cholinergic, as well as the non-cholinergic components of the EFS-evoked contractions in isolated rat urinary bladders, are modulated by some cyclo-oxygenase formed AA metabolites, particularly certain PGs, like PGE2. © 1987.
format JOUR
author Dveksler, G.
Gimeno, M.F.
Gimeno, A.L.
author_facet Dveksler, G.
Gimeno, M.F.
Gimeno, A.L.
author_sort Dveksler, G.
title Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
title_short Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
title_full Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
title_fullStr Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
title_full_unstemmed Cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. Influence of some eicosanoids
title_sort cholinergic and non-cholinergic components of the inotropism evoked by electric field stimulation in the isolated rat urinary bladder. influence of some eicosanoids
url http://hdl.handle.net/20.500.12110/paper_00316989_v19_n4_p295_Dveksler
work_keys_str_mv AT dvekslerg cholinergicandnoncholinergiccomponentsoftheinotropismevokedbyelectricfieldstimulationintheisolatedraturinarybladderinfluenceofsomeeicosanoids
AT gimenomf cholinergicandnoncholinergiccomponentsoftheinotropismevokedbyelectricfieldstimulationintheisolatedraturinarybladderinfluenceofsomeeicosanoids
AT gimenoal cholinergicandnoncholinergiccomponentsoftheinotropismevokedbyelectricfieldstimulationintheisolatedraturinarybladderinfluenceofsomeeicosanoids
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