Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco
We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a...
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todo:paper_00311820_v139_n12_p1570_Maffey2023-10-03T14:41:15Z Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco Maffey, L. Cardinal, M.V. Ordóñez-Krasnowski, P.C. Lanati, L.A. Lauricella, M.A. Schijman, A.G. Gürtler, R.E. Chagas disease Discrete Typing Units PCR Triatoma infestans Triatoma sordida Trypanosoma cruzi protozoal DNA ampoule Argentina article Chagas disease controlled study field study human nonhuman opossum parasite identification polymerase chain reaction priority journal rectum rural area Triatoma Triatoma infestans Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Dogs Enzyme-Linked Immunosorbent Assay Genotype Humans Insect Vectors Polymerase Chain Reaction Triatoma Trypanosoma cruzi We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle. © Copyright © Cambridge University Press 2012. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00311820_v139_n12_p1570_Maffey |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Discrete Typing Units PCR Triatoma infestans Triatoma sordida Trypanosoma cruzi protozoal DNA ampoule Argentina article Chagas disease controlled study field study human nonhuman opossum parasite identification polymerase chain reaction priority journal rectum rural area Triatoma Triatoma infestans Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Dogs Enzyme-Linked Immunosorbent Assay Genotype Humans Insect Vectors Polymerase Chain Reaction Triatoma Trypanosoma cruzi |
| spellingShingle |
Chagas disease Discrete Typing Units PCR Triatoma infestans Triatoma sordida Trypanosoma cruzi protozoal DNA ampoule Argentina article Chagas disease controlled study field study human nonhuman opossum parasite identification polymerase chain reaction priority journal rectum rural area Triatoma Triatoma infestans Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Dogs Enzyme-Linked Immunosorbent Assay Genotype Humans Insect Vectors Polymerase Chain Reaction Triatoma Trypanosoma cruzi Maffey, L. Cardinal, M.V. Ordóñez-Krasnowski, P.C. Lanati, L.A. Lauricella, M.A. Schijman, A.G. Gürtler, R.E. Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| topic_facet |
Chagas disease Discrete Typing Units PCR Triatoma infestans Triatoma sordida Trypanosoma cruzi protozoal DNA ampoule Argentina article Chagas disease controlled study field study human nonhuman opossum parasite identification polymerase chain reaction priority journal rectum rural area Triatoma Triatoma infestans Triatoma sordida Trypanosoma cruzi Animals Animals, Domestic Argentina Dogs Enzyme-Linked Immunosorbent Assay Genotype Humans Insect Vectors Polymerase Chain Reaction Triatoma Trypanosoma cruzi |
| description |
We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle. © Copyright © Cambridge University Press 2012. |
| format |
JOUR |
| author |
Maffey, L. Cardinal, M.V. Ordóñez-Krasnowski, P.C. Lanati, L.A. Lauricella, M.A. Schijman, A.G. Gürtler, R.E. |
| author_facet |
Maffey, L. Cardinal, M.V. Ordóñez-Krasnowski, P.C. Lanati, L.A. Lauricella, M.A. Schijman, A.G. Gürtler, R.E. |
| author_sort |
Maffey, L. |
| title |
Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| title_short |
Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| title_full |
Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| title_fullStr |
Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| title_full_unstemmed |
Direct molecular identification of Trypanosoma cruzi Discrete Typing Units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco |
| title_sort |
direct molecular identification of trypanosoma cruzi discrete typing units in domestic and peridomestic triatoma infestans and triatoma sordida from the argentine chaco |
| url |
http://hdl.handle.net/20.500.12110/paper_00311820_v139_n12_p1570_Maffey |
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