Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi
We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. S...
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todo:paper_00311820_v115_n6_p563_Araya2023-10-03T14:41:12Z Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi Araya, J. Cano, M.I. Gomes, H.B.M. Novak, E.M. Requena, J.M. Alonso, C. Levin, M.J. Guevara, P. Ramirez, J.L. Franco Da Silveira, J. Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi. strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00311820_v115_n6_p563_Araya |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
spellingShingle |
Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Araya, J. Cano, M.I. Gomes, H.B.M. Novak, E.M. Requena, J.M. Alonso, C. Levin, M.J. Guevara, P. Ramirez, J.L. Franco Da Silveira, J. Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
topic_facet |
Diagnostic tool Genomic distribution Polymorphic marker Sequence analysis Trypanosoma cruzi repetitive DNA article dna sequence gene location nonhuman nucleotide repeat parasite identification priority journal sequence analysis sequence homology species difference trypanosoma cruzi Animals Base Sequence Blotting, Southern Chagas Disease DNA, Protozoan Electrophoresis, Gel, Pulsed-Field Genome, Protozoan Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi |
description |
We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi. strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome. |
format |
JOUR |
author |
Araya, J. Cano, M.I. Gomes, H.B.M. Novak, E.M. Requena, J.M. Alonso, C. Levin, M.J. Guevara, P. Ramirez, J.L. Franco Da Silveira, J. |
author_facet |
Araya, J. Cano, M.I. Gomes, H.B.M. Novak, E.M. Requena, J.M. Alonso, C. Levin, M.J. Guevara, P. Ramirez, J.L. Franco Da Silveira, J. |
author_sort |
Araya, J. |
title |
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
title_short |
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
title_full |
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
title_fullStr |
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
title_full_unstemmed |
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi |
title_sort |
characterization of an interspersed repetitive dna element in the genome of trypanosoma cruzi |
url |
http://hdl.handle.net/20.500.12110/paper_00311820_v115_n6_p563_Araya |
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